Detailed information NTG0633764

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRX85_RS13500
• Genome accession: NZ_CP088894
• Coordinates: 2834867..2835985 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain XH1935
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2829867..2840985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRX85_RS13490 (LRX85_13490)	-	2832983..2833675 (-)	693	WP_002124845.1	YggS family pyridoxal phosphate-dependent enzyme	-
LRX85_RS13495 (LRX85_13495)	pilT	2833802..2834839 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
LRX85_RS13500 (LRX85_13500)	pilU	2834867..2835985 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LRX85_RS13505 (LRX85_13505)	fur	2836055..2836492 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
LRX85_RS13510 (LRX85_13510)	-	2836604..2837002 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
LRX85_RS13515 (LRX85_13515)	-	2837057..2837380 (-)	324	WP_000442589.1	RnfH family protein	-
LRX85_RS13520 (LRX85_13520)	-	2837380..2838447 (-)	1068	WP_004835750.1	hypothetical protein	-
LRX85_RS13525 (LRX85_13525)	-	2838600..2839055 (-)	456	WP_029574892.1	bacteriohemerythrin	-
LRX85_RS13530 (LRX85_13530)	-	2839307..2840155 (-)	849	WP_029574891.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=633764 LRX85_RS13500 WP_000347036.1 2834867..2835985(+) (pilU) [Acinetobacter baumannii strain XH1935]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=633764 LRX85_RS13500 WP_000347036.1 2834867..2835985(+) (pilU) [Acinetobacter baumannii strain XH1935]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392