Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KDJ21_RS13260 Genome accession   NZ_CP088268
Coordinates   2717481..2718548 (+) Length   355 a.a.
NCBI ID   WP_212136683.1    Uniprot ID   -
Organism   Metabacillus litoralis strain NCTR108     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2712481..2723548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KDJ21_RS13225 (KDJ21_013225) - 2713260..2714390 (+) 1131 WP_212136685.1 hypothetical protein -
  KDJ21_RS13230 (KDJ21_013230) - 2714405..2714713 (+) 309 WP_121662829.1 MTH1187 family thiamine-binding protein -
  KDJ21_RS13235 (KDJ21_013235) - 2714772..2714945 (-) 174 WP_098799532.1 DUF2759 domain-containing protein -
  KDJ21_RS13240 (KDJ21_013240) - 2715199..2715831 (+) 633 WP_121662828.1 MBL fold metallo-hydrolase -
  KDJ21_RS13245 (KDJ21_013245) - 2715894..2716142 (-) 249 WP_098799530.1 DUF2626 domain-containing protein -
  KDJ21_RS13250 (KDJ21_013250) - 2716482..2716862 (+) 381 WP_269671069.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  KDJ21_RS13260 (KDJ21_013260) comGA 2717481..2718548 (+) 1068 WP_212136683.1 competence type IV pilus ATPase ComGA Machinery gene
  KDJ21_RS13265 (KDJ21_013265) - 2718538..2719017 (+) 480 WP_232273269.1 type II secretion system F family protein -
  KDJ21_RS13270 (KDJ21_013270) - 2719065..2719571 (+) 507 WP_269671070.1 type II secretion system F family protein -
  KDJ21_RS13275 (KDJ21_013275) comGC 2719582..2719896 (+) 315 WP_212136679.1 competence type IV pilus major pilin ComGC Machinery gene
  KDJ21_RS13280 (KDJ21_013280) comGD 2719871..2720326 (+) 456 WP_212136677.1 competence type IV pilus minor pilin ComGD -
  KDJ21_RS13285 (KDJ21_013285) comGE 2720292..2720639 (+) 348 WP_212136675.1 competence type IV pilus minor pilin ComGE -
  KDJ21_RS13290 (KDJ21_013290) comGF 2720560..2721066 (+) 507 WP_269671071.1 competence type IV pilus minor pilin ComGF -
  KDJ21_RS13295 (KDJ21_013295) comGG 2721063..2721443 (+) 381 WP_212136673.1 competence type IV pilus minor pilin ComGG -
  KDJ21_RS13300 (KDJ21_013300) - 2721485..2722000 (+) 516 WP_212136671.1 shikimate kinase -
  KDJ21_RS13305 (KDJ21_013305) - 2722036..2722230 (+) 195 WP_212136669.1 YqzE family protein -
  KDJ21_RS13310 (KDJ21_013310) - 2722365..2722679 (-) 315 WP_121662817.1 DUF3889 domain-containing protein -
  KDJ21_RS13315 (KDJ21_013315) - 2722802..2723032 (+) 231 WP_212136667.1 hypothetical protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 40457.95 Da        Isoelectric Point: 8.6638

>NTDB_id=633637 KDJ21_RS13260 WP_212136683.1 2717481..2718548(+) (comGA) [Metabacillus litoralis strain NCTR108]
MKPIEQISERIIEEACALRASDIHIIPREQDTLIQFRVDDDLIEKHTMKKEICGRIISHLKFLASMDIGERRRPQNGSLS
LSTTNLNVHLRLSTLPTIHEESLVIRILPQEKIPPITHLSLFPATTNKLLSILNHSHGLMIFTGPTGSGKTTTLYSLIHY
AKRHFNRNIITLEDPVETKSEEVLQVQVNEKAGITYAAGLKAILRHDPDIIMVGEIRDRETAQIAIRASLTGHLVLSTMH
TRDAKGAIYRLMEFDVSLSEIEQTLIAVSAQRLVQLKCPFCEDQCSPFCKKLRQKRRLSIYELLYGKNLTAVVKEAKGEL
TSYQYPTLKDVIKKGIALGYLYPNSYDRWVFRYED

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=633637 KDJ21_RS13260 WP_212136683.1 2717481..2718548(+) (comGA) [Metabacillus litoralis strain NCTR108]
GTGAAACCAATCGAACAAATAAGTGAACGCATTATTGAAGAAGCCTGTGCATTAAGAGCCTCAGACATTCACATTATTCC
TAGAGAACAAGACACGCTCATTCAATTTCGAGTTGATGATGATTTAATTGAAAAGCATACAATGAAAAAAGAAATCTGTG
GTCGTATTATCTCTCATCTAAAATTCTTAGCCTCCATGGACATTGGTGAAAGACGAAGACCACAAAATGGGTCACTGTCA
CTTTCTACTACTAATCTGAACGTTCATCTTAGATTATCCACCTTACCTACCATTCACGAAGAAAGTCTTGTGATTAGAAT
TCTGCCACAAGAGAAAATTCCACCAATTACACATTTATCGTTATTCCCTGCCACAACTAACAAGCTGCTCTCTATTTTGA
ATCACTCACATGGTTTGATGATTTTTACAGGTCCAACTGGTTCGGGTAAGACAACTACACTTTATTCTCTCATTCATTAT
GCAAAGCGTCATTTTAACAGAAATATTATTACCTTAGAGGACCCGGTTGAAACGAAAAGTGAGGAAGTATTGCAAGTTCA
AGTTAATGAAAAAGCGGGGATTACCTATGCTGCTGGTTTAAAGGCAATTCTGCGTCATGATCCCGATATTATTATGGTTG
GAGAAATTCGAGATCGAGAAACAGCTCAAATAGCCATTCGTGCCAGTCTAACTGGTCACCTTGTTTTGTCAACCATGCAT
ACAAGAGATGCAAAGGGTGCGATATATCGTTTAATGGAATTTGATGTGAGTTTATCAGAAATTGAACAAACCCTCATTGC
TGTATCAGCTCAGAGATTGGTTCAACTTAAATGTCCATTTTGTGAAGATCAATGCTCTCCTTTTTGTAAGAAACTAAGAC
AAAAAAGAAGGCTAAGCATTTATGAGCTTTTATATGGGAAAAACTTAACAGCTGTAGTAAAGGAGGCGAAAGGGGAATTA
ACTTCCTATCAATACCCAACATTAAAAGATGTTATAAAAAAGGGAATAGCACTAGGATATCTTTATCCAAATTCTTATGA
CCGTTGGGTGTTTCGTTATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.416

100

0.676
  pilB Glaesserella parasuis strain SC1401

37.535

100

0.377