Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LPC09_RS17370 Genome accession   NZ_CP088245
Coordinates   3410541..3411608 (-) Length   355 a.a.
NCBI ID   WP_231307892.1    Uniprot ID   -
Organism   Metabacillus sp. B2-18     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3405541..3416608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPC09_RS17320 (LPC09_17320) - 3406056..3406268 (-) 213 WP_098799162.1 hypothetical protein -
  LPC09_RS17325 (LPC09_17325) - 3406395..3406709 (+) 315 WP_121662817.1 DUF3889 domain-containing protein -
  LPC09_RS17330 (LPC09_17330) - 3406859..3407053 (-) 195 WP_231307885.1 YqzE family protein -
  LPC09_RS17335 (LPC09_17335) - 3407106..3407603 (-) 498 WP_231307886.1 shikimate kinase -
  LPC09_RS17340 (LPC09_17340) comGG 3407645..3408025 (-) 381 WP_231307887.1 competence type IV pilus minor pilin ComGG -
  LPC09_RS17345 (LPC09_17345) comGF 3408022..3408525 (-) 504 WP_269217398.1 competence type IV pilus minor pilin ComGF -
  LPC09_RS17350 (LPC09_17350) comGE 3408449..3408796 (-) 348 WP_231307888.1 competence type IV pilus minor pilin ComGE -
  LPC09_RS17355 (LPC09_17355) comGD 3408762..3409217 (-) 456 WP_231307889.1 competence type IV pilus minor pilin ComGD -
  LPC09_RS17360 (LPC09_17360) comGC 3409192..3409506 (-) 315 WP_231307890.1 competence type IV pilus major pilin ComGC Machinery gene
  LPC09_RS17365 (LPC09_17365) comGB 3409517..3410551 (-) 1035 WP_231307891.1 competence type IV pilus assembly protein ComGB -
  LPC09_RS17370 (LPC09_17370) comGA 3410541..3411608 (-) 1068 WP_231307892.1 competence type IV pilus ATPase ComGA Machinery gene
  LPC09_RS17380 (LPC09_17380) - 3412292..3412672 (-) 381 WP_098799529.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LPC09_RS17385 (LPC09_17385) - 3413015..3413263 (+) 249 WP_098799530.1 DUF2626 domain-containing protein -
  LPC09_RS17390 (LPC09_17390) - 3413328..3413960 (-) 633 WP_231307893.1 MBL fold metallo-hydrolase -
  LPC09_RS17395 (LPC09_17395) - 3414212..3414385 (+) 174 WP_098799532.1 DUF2759 domain-containing protein -
  LPC09_RS17400 (LPC09_17400) - 3414445..3414753 (-) 309 WP_098799533.1 MTH1187 family thiamine-binding protein -
  LPC09_RS17405 (LPC09_17405) - 3414768..3415898 (-) 1131 WP_098799534.1 hypothetical protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 40427.93 Da        Isoelectric Point: 8.6638

>NTDB_id=633476 LPC09_RS17370 WP_231307892.1 3410541..3411608(-) (comGA) [Metabacillus sp. B2-18]
MKPIEQISERIIEEACALRASDIHIIPREQDTLIQFRVDDDLIEKHTMKKEICGRIISHLKFLASMDIGERRRPQNGSLS
LSTTNLNVHLRLSTLPTIHEESLVIRILPQEKIPPITHLSLFPATTNKLLSILNHSHGLMIFTGPTGSGKTTTLYSLIHY
AKRHFNRNIITLEDPVETKSEEVLQVQVNEKAGITYAAGLKAILRHDPDIIMVGEIRDRETAQIAIRASLTGHLVLSTMH
TRDAKGAIYRLMEFDVSLSEIEQTLIAVSAQRLVQLKCPFCEDQCSPFCKKLRQKRRLSIYELLYGKNLTAVVKEAKGEL
ASYQYPTLKDVIKKGIALGYLYPNSYDRWVFRYED

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=633476 LPC09_RS17370 WP_231307892.1 3410541..3411608(-) (comGA) [Metabacillus sp. B2-18]
GTGAAACCAATTGAACAAATCAGTGAACGTATTATAGAAGAAGCCTGTGCATTAAGAGCCTCAGACATTCACATTATTCC
CAGAGAACAAGACACACTCATTCAGTTTCGAGTTGATGATGATTTAATTGAAAAACATACGATGAAAAAAGAGATCTGTG
GTCGGATCATCTCTCATCTAAAATTTCTGGCTTCGATGGATATTGGAGAAAGACGAAGACCTCAGAATGGATCACTATCA
CTTTCTACTACTAATCTAAATGTTCATCTAAGATTATCCACCCTCCCTACCATTCACGAAGAAAGTCTTGTGATTAGAAT
TTTGCCACAAGAGAAAATACCGCCAATAACACATTTATCATTGTTTCCTGCCACAACAAACAAACTGCTCTCCATTTTGA
ATCACTCACATGGTTTAATGATTTTTACCGGCCCAACTGGCTCAGGTAAAACAACAACACTTTATTCTCTCATTCATTAT
GCAAAGCGTCATTTTAACAGAAATATTATCACGTTAGAGGATCCGGTAGAAACGAAAAGTGAGGAAGTATTGCAAGTTCA
AGTTAATGAAAAAGCGGGGATTACCTATGCTGCTGGGTTAAAGGCAATTCTGCGTCATGATCCTGATATTATTATGGTTG
GAGAAATACGAGATCGAGAAACAGCTCAAATTGCGATTCGCGCCAGTCTAACGGGTCATCTTGTTTTATCAACCATGCAT
ACAAGAGATGCAAAAGGAGCGATATATCGATTAATGGAATTCGATGTAAGTTTATCTGAAATTGAACAAACTCTAATTGC
GGTCTCAGCTCAAAGACTAGTTCAACTTAAATGTCCATTCTGTGAAGATCAATGCTCTCCTTTTTGTAAGAAGCTAAGAC
AAAAAAGAAGGTTAAGTATCTATGAGCTTTTATATGGAAAAAACCTAACAGCTGTAGTAAAGGAGGCGAAAGGAGAATTA
GCTTCCTACCAATACCCAACATTAAAAGATGTTATTAAAAAGGGAATTGCACTAGGATATCTTTATCCAAATTCTTATGA
CCGTTGGGTGTTTCGTTATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.697

100

0.679
  pilB Glaesserella parasuis strain SC1401

37.815

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.681

97.183

0.366