Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LOC77_RS01755 Genome accession   NZ_CP087957
Coordinates   321661..322098 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain B-001     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 316661..327098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOC77_RS01715 - 317240..317404 (-) 165 Protein_297 molybdopterin dinucleotide binding domain-containing protein -
  LOC77_RS01720 - 317518..318720 (+) 1203 WP_057080005.1 GTP-binding protein -
  LOC77_RS01725 - 318785..319921 (+) 1137 WP_230639731.1 zinc-dependent alcohol dehydrogenase -
  LOC77_RS01730 - 319936..320370 (+) 435 WP_057080003.1 RDD family protein -
  LOC77_RS01735 - 320442..320762 (+) 321 WP_044053437.1 YckD family protein -
  LOC77_RS01740 - 320866..320982 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  LOC77_RS01745 - 320981..321200 (+) 220 Protein_303 family 1 glycosylhydrolase -
  LOC77_RS01750 nin/comJ 321241..321639 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  LOC77_RS01755 nucA/comI 321661..322098 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LOC77_RS01760 hxlB 322457..323014 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  LOC77_RS01765 hxlA 323011..323646 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  LOC77_RS01770 - 323878..324240 (+) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=632008 LOC77_RS01755 WP_003156588.1 321661..322098(-) (nucA/comI) [Bacillus velezensis strain B-001]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=632008 LOC77_RS01755 WP_003156588.1 321661..322098(-) (nucA/comI) [Bacillus velezensis strain B-001]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641