Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EcoO8H8_RS15145 Genome accession   NZ_AP019856
Coordinates   3179252..3180061 (-) Length   269 a.a.
NCBI ID   WP_000895880.1    Uniprot ID   -
Organism   Escherichia coli O8:H8 strain 16F5M1D1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3174252..3185061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EcoO8H8_RS15130 (Eco16F5M1D1_3008) gspD 3175709..3177769 (-) 2061 WP_000498824.1 type II secretion system secretin GspD -
  EcoO8H8_RS15135 (Eco16F5M1D1_3009) gspC 3177799..3178758 (-) 960 WP_000135071.1 type II secretion system protein GspC -
  EcoO8H8_RS15140 (Eco16F5M1D1_3010) gspS2 3178776..3179186 (-) 411 WP_001298744.1 type II secretion system pilot lipoprotein GspS-beta -
  EcoO8H8_RS15145 (Eco16F5M1D1_3011) pilD 3179252..3180061 (-) 810 WP_000895880.1 prepilin peptidase PppA Machinery gene
  EcoO8H8_RS15150 (Eco16F5M1D1_3012) sslE 3180191..3184753 (-) 4563 WP_096307163.1 lipoprotein metalloprotease SslE -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29456.10 Da        Isoelectric Point: 8.3829

>NTDB_id=63131 EcoO8H8_RS15145 WP_000895880.1 3179252..3180061(-) (pilD) [Escherichia coli O8:H8 strain 16F5M1D1]
MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAI
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=63131 EcoO8H8_RS15145 WP_000895880.1 3179252..3180061(-) (pilD) [Escherichia coli O8:H8 strain 16F5M1D1]
ATGCTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGTCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTGCCGCGTTCGCACTGTCCGCATTGCCAGCAGACTATCCGCGTTCGTGACAATATTCCG
CTGCTCTCCTGGTTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCATCATCGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTGATTGCGGCATGGGCGCAACAGAGTCCGTTAACGCTACAAGACGCAGTTACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTGGCTGGGTGGGGCCGTTATCACTGCCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCATT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-240)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

46.067

99.257

0.457
  pilD Vibrio campbellii strain DS40M4

42.066

100

0.424
  pilD Acinetobacter nosocomialis M2

38.491

98.513

0.379
  pilD Neisseria gonorrhoeae MS11

39.689

95.539

0.379
  pilD Acinetobacter baumannii D1279779

38.113

98.513

0.375

Multiple sequence alignment