Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NQS37_RS04465 Genome accession   NZ_CP104498
Coordinates   940277..941833 (-) Length   518 a.a.
NCBI ID   WP_069079328.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain Sw698     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 935277..946833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQS37_RS25085 - 935975..936076 (-) 102 Protein_886 preprotein translocase subunit SecF -
  NQS37_RS04450 (NQS37_04450) - 936111..937397 (-) 1287 WP_020371584.1 hypothetical protein -
  NQS37_RS04455 (NQS37_04455) - 937473..938756 (-) 1284 WP_016724399.1 hypothetical protein -
  NQS37_RS04460 (NQS37_04460) gspF 939064..940275 (-) 1212 WP_011003007.1 type II secretion system inner membrane protein GspF -
  NQS37_RS04465 (NQS37_04465) pilF 940277..941833 (-) 1557 WP_069079328.1 type II secretion system ATPase GspE Machinery gene
  NQS37_RS04470 (NQS37_04470) gspD 941868..944288 (-) 2421 WP_021154965.1 type II secretion system secretin GspD -
  NQS37_RS04475 (NQS37_04475) - 944322..945173 (-) 852 WP_019718954.1 type II secretion system protein N -
  NQS37_RS04480 (NQS37_04480) gspM 945177..945755 (-) 579 WP_011003003.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 518 a.a.        Molecular weight: 55861.86 Da        Isoelectric Point: 5.5004

>NTDB_id=629845 NQS37_RS04465 WP_069079328.1 940277..941833(-) (pilF) [Ralstonia pseudosolanacearum strain Sw698]
MATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLEVWVCPETSRAALAELARVFGALRLVSLDAAAISAA
TQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFR
VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDL
GKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRLDASSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFA
KALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRL
CVHCRREEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTVTPEIQTMIHRQAPES
EIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD

Nucleotide


Download         Length: 1557 bp        

>NTDB_id=629845 NQS37_RS04465 WP_069079328.1 940277..941833(-) (pilF) [Ralstonia pseudosolanacearum strain Sw698]
ATGGCAACGCAAGCTTCTTCCACTGACGCGCGCACGCTCGAGCCGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTATGT
GTTCGCGCGCGACGCCAGGCTGCTGGTGGCGCGGCAGGATGTCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGC
GCGCCGCGCTGGCCGAGCTGGCCCGCGTGTTCGGCGCGCTGCGCCTGGTGAGCCTGGACGCCGCCGCGATCTCCGCCGCC
ACGCAGACGGCCTACAACGCCCAGGACGGCAGCGCGGCCCAGGTGGTCGGCGAGGTCGAGGGCGAGGTGGACCTGTCGCG
CCTGATGCAGGACATCCCGGCCGTGGAAGACCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGC
TGCTCACGCAGGCCGCACGCGAGGGCGCCTCGGACATCCACATCGAGCCGTTCGAGAACGCCTCGGTGGTGCGCTTCCGC
GTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGC
GCAGCTCGACATCGCCGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTGGGCGGGCGACCGGTGGACGTGC
GTGTCTCCACCCTGCCGACCGGCCACGGCGAGCGCGCGGTGCTGCGCCTGCTCGACAAGGAAGCCGGCCGGCTTGACCTC
GGCAAGCTCGGCATGGGCGCCGGCACGCTGGGGCGCTTCGACCGCCTGATCAACCAGCCGCACGGCATCGTGCTCGTCAC
CGGCCCGACCGGCTCGGGCAAGACCACCACGCTGTATGCCGCGCTGTCGCGGCTCGATGCCAGCAGCACCAACATCATGA
CGGTGGAGGACCCGATCGAGTACGACCTGGACGGTATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCC
AAGGCCCTGCGCGCCATCCTGCGGCAGGACCCGGACGTGGTGATGATCGGGGAGATCCGCGACCTGGAGACCGCGCAGAT
CGCGGTGCAGGCCTCGCTGACCGGCCACCTGGTGCTGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGC
TGATCGACATGGGGATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTG
TGCGTGCACTGCCGCCGCGAGGAAGTGCTGGAGCTCACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCCGA
CGGCACCGCCGCCGCCGTGCCGACGCGCCGCTCGGTGTGGCACCACGTCGGCTGCGAACACTGCGGCCAATCGGGCTACC
AGGGCCGGACCGGCGTGTACGAGCTGCTGACGGTGACGCCCGAGATCCAGACCATGATCCACCGCCAGGCGCCCGAGTCC
GAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCATACCATGCGCGCGGATGCGCAGCGGTGGCTCGACGCGGGCGCGAC
GTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGACTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

42.744

97.104

0.415
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.689

99.228

0.394
  pilB Vibrio cholerae strain A1552

45.434

86.68

0.394
  pilB Vibrio campbellii strain DS40M4

44.615

87.838

0.392
  pilB/pilB1 Synechocystis sp. PCC 6803

44.676

83.398

0.373
  pilB Vibrio parahaemolyticus RIMD 2210633

46.324

78.764

0.365