NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	LOZ87_RS12205	Genome accession	NZ_CP087391
Coordinates	2516219..2516833 (-)	Length	204 a.a.
NCBI ID	WP_020956097.1	Uniprot ID	-
Organism	Bacillus amyloliquefaciens strain ZF57
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2511219..2521833

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOZ87_RS12175 (LOZ87_12175)	rpsT	2511522..2511788 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LOZ87_RS12180 (LOZ87_12180)	holA	2511805..2512846 (-)	1042	Protein_2355	DNA polymerase III subunit delta	-
LOZ87_RS12185 (LOZ87_12185)	-	2512881..2513037 (-)	157	Protein_2356	hypothetical protein	-
LOZ87_RS12190 (LOZ87_12190)	-	2513078..2513212 (+)	135	WP_003152870.1	YqzM family protein	-
LOZ87_RS12195 (LOZ87_12195)	comEC	2513231..2515582 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LOZ87_RS12200 (LOZ87_12200)	-	2515583..2516152 (-)	570	WP_007408264.1	ComE operon protein 2	-
LOZ87_RS12205 (LOZ87_12205)	comEA	2516219..2516833 (-)	615	WP_020956097.1	helix-hairpin-helix domain-containing protein	Machinery gene
LOZ87_RS12210 (LOZ87_12210)	comER	2516892..2517713 (+)	822	WP_020956098.1	late competence protein ComER	-
LOZ87_RS12215 (LOZ87_12215)	-	2517783..2518520 (-)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
LOZ87_RS12220 (LOZ87_12220)	rsfS	2518517..2518873 (-)	357	WP_007408260.1	ribosome silencing factor	-
LOZ87_RS12225 (LOZ87_12225)	yqeK	2518891..2519451 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LOZ87_RS12230 (LOZ87_12230)	-	2519441..2520010 (-)	570	WP_039063329.1	nicotinate-nucleotide adenylyltransferase	-
LOZ87_RS12235 (LOZ87_12235)	yhbY	2520021..2520311 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LOZ87_RS12240 (LOZ87_12240)	aroE	2520305..2521147 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22251.45 Da Isoelectric Point: 6.4940

                                >NTDB_id=629283 LOZ87_RS12205 WP_020956097.1 2516219..2516833(-) (comEA) [Bacillus amyloliquefaciens strain ZF57]

MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=629283 LOZ87_RS12205 WP_020956097.1 2516219..2516833(-) (comEA) [Bacillus amyloliquefaciens strain ZF57]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT

TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363

Detailed information NTG0629283