Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   N4502_RS15395 Genome accession   NZ_CP104371
Coordinates   3184171..3186369 (-) Length   732 a.a.
NCBI ID   WP_001306641.1    Uniprot ID   -
Organism   Escherichia coli strain 2021CK-01784     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3179171..3191369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4502_RS15375 (N4502_15375) hslU 3179975..3181306 (-) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -
  N4502_RS15380 (N4502_15380) hslV 3181316..3181846 (-) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  N4502_RS15385 (N4502_15385) ftsN 3181939..3182898 (-) 960 WP_000068828.1 cell division protein FtsN -
  N4502_RS15390 (N4502_15390) cytR 3182990..3184015 (-) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  N4502_RS15395 (N4502_15395) priA 3184171..3186369 (-) 2199 WP_001306641.1 primosomal protein N' Machinery gene
  N4502_RS15400 (N4502_15400) rpmE 3186572..3186784 (+) 213 WP_000710769.1 50S ribosomal protein L31 -
  N4502_RS15405 (N4502_15405) yiiX 3186845..3187453 (-) 609 WP_000702300.1 YiiX family permuted papain-like enzyme -
  N4502_RS15410 (N4502_15410) metJ 3187513..3187830 (-) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  N4502_RS15415 (N4502_15415) metB 3188107..3189267 (+) 1161 WP_000197201.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81697.08 Da        Isoelectric Point: 9.3699

>NTDB_id=629085 N4502_RS15395 WP_001306641.1 3184171..3186369(-) (priA) [Escherichia coli strain 2021CK-01784]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRNQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKLQ
AGLAPSLITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=629085 N4502_RS15395 WP_001306641.1 3184171..3186369(-) (priA) [Escherichia coli strain 2021CK-01784]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTCAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTATCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGTTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTATGGGCGGCGGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCGCCAAAGCAACAACAGGCACTGGCGGCATTGCGGCAAGGCAAAATCTGGCGCAACCAAGTTGCCACGCTCGAATTT
AATGATGCCGCGTTGCAAGCACTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGTGACTGGCG
AAAGAATTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACTGAACAGGCCACCGCCGTTGGCGCAATTCATAGTGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGCTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGCGCGCATAG
CGAGCAAATCCCAATTATTCTTGGCTCCGCAACGCCCGCACTGGAAACGTTATGCAACGTGCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCGGGCAATGCGCGCCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGCTGCAG
GCAGGTCTGGCTCCGTCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAATCG
TCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGTTGCCACCACTGTGACAGCCAACGCCCTGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTTGCGCCGCTGTTCCCTGGTGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTTGAACAACAACTGGCGGAAGTACATCGCGGCGGTGCGA
GGATTTTGATCGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCATTACTGGACGTAGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGTGAAGTGGTGTTGCAAACGCACCATCCGGAACATCCTCTCTTGCAAACATTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAACGACGCATGATGCAACTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCAGAAGATCATAACAATCAGCACGCGCCGCTGTTCCTGCAACAGTTGCGTAATCTGATCCTCGCCAGCCCGCTTGCAGA
CGACAAACTGTGGGTTCTCGGTCCGGTTCCGGCACTGGCCCCTAAGCGTGGTGGCCGCTGGCGCTGGCAAATTTTGTTGC
AGCACCCTTCCCGCGTGCGATTACAGCACATCATCAATGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(203-362)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.912

100

0.452