Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOY54_RS01340 Genome accession   NZ_CP087180
Coordinates   299087..300115 (+) Length   342 a.a.
NCBI ID   WP_258608908.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-032     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 294087..305115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY54_RS01310 (LOY54_01310) - 294524..294958 (-) 435 WP_195885461.1 DUF4426 domain-containing protein -
  LOY54_RS01315 (LOY54_01315) metW 294983..295603 (-) 621 WP_038997652.1 methionine biosynthesis protein MetW -
  LOY54_RS01320 (LOY54_01320) - 295611..296750 (-) 1140 WP_045193963.1 homoserine O-acetyltransferase -
  LOY54_RS01325 (LOY54_01325) - 296876..297466 (-) 591 WP_045193962.1 YggT family protein -
  LOY54_RS01330 (LOY54_01330) proC 297477..298295 (-) 819 WP_195885462.1 pyrroline-5-carboxylate reductase -
  LOY54_RS01335 (LOY54_01335) - 298334..299020 (-) 687 WP_093377203.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOY54_RS01340 (LOY54_01340) pilT 299087..300115 (+) 1029 WP_258608908.1 type IV pilus twitching motility protein PilT Machinery gene
  LOY54_RS01345 (LOY54_01345) - 300112..300747 (-) 636 WP_045193958.1 C40 family peptidase -
  LOY54_RS01350 (LOY54_01350) - 300994..301413 (+) 420 WP_045193957.1 TM2 domain-containing protein -
  LOY54_RS01355 (LOY54_01355) - 301462..302151 (-) 690 WP_038997644.1 SPOR domain-containing protein -
  LOY54_RS01360 (LOY54_01360) argS 302153..303889 (-) 1737 WP_258608909.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 36548.06 Da        Isoelectric Point: 6.3424

>NTDB_id=627447 LOY54_RS01340 WP_258608908.1 299087..300115(+) (pilT) [Pseudomonas sp. B21-032]
MDVTELLALAVQAGASDLHLAAGEVPALRFGGCLRPLELPALTPQDMDEMLGQVMSAAQQKDFETHLDIDFAFAVPALAR
FRVNAFQQLHGAAAVLRVIPAQVSSLAELDLGDVFRQIAELSGGLVLVTGPTGSGKSTTLAALVDHLNRERALHIVTLED
PIEFIHSSQRSRISQREIGRHTRGFAQALRAALRQDPDVIMLGELRDPQSIRLALTAAETGHLVLATVHTGSAAKTIDRL
VDVFAAEEKQLVRAMLAESLQAVVSQVLVEKVGGGRLAAHEILLATPAVRNLVREGKVAQIYSAIQTGGAQGMQTLDACL
QGLVRQGLVSPESARERALGRF

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=627447 LOY54_RS01340 WP_258608908.1 299087..300115(+) (pilT) [Pseudomonas sp. B21-032]
ATGGATGTGACTGAACTGCTGGCGCTGGCTGTGCAAGCCGGTGCCAGTGACCTGCACCTGGCGGCGGGCGAGGTGCCGGC
GCTGCGTTTTGGGGGGTGTTTGCGGCCGCTGGAGTTGCCGGCGCTGACGCCGCAAGACATGGACGAAATGCTCGGACAGG
TGATGTCCGCGGCGCAGCAAAAGGATTTTGAAACACATCTGGATATCGACTTTGCCTTTGCCGTGCCAGCATTGGCGCGC
TTTCGGGTCAATGCTTTCCAGCAACTGCATGGCGCCGCTGCAGTGCTGCGGGTGATTCCGGCGCAGGTCAGCAGCCTGGC
AGAACTCGACCTGGGGGATGTGTTTCGGCAAATTGCCGAGCTGTCGGGCGGGCTGGTGCTGGTTACCGGGCCCACAGGGT
CGGGCAAATCGACCACCCTGGCGGCTCTGGTCGATCACCTGAACCGTGAGCGGGCGCTGCACATCGTCACCCTCGAGGAC
CCCATCGAATTTATCCACAGCTCGCAGCGCAGCCGCATCAGCCAGCGCGAGATCGGCCGGCACACCCGGGGTTTTGCCCA
GGCCTTGCGCGCAGCATTACGCCAGGATCCGGATGTGATCATGCTCGGTGAACTGCGCGATCCGCAGAGCATTCGCCTGG
CCCTGACTGCAGCGGAAACCGGGCACCTGGTGCTGGCCACGGTGCATACCGGCTCGGCGGCGAAAACCATTGACCGCCTG
GTGGATGTGTTCGCCGCCGAAGAGAAGCAACTGGTACGCGCCATGCTCGCCGAGTCGCTGCAGGCAGTGGTCTCGCAGGT
TCTGGTGGAGAAGGTTGGCGGCGGCCGGCTGGCAGCCCACGAGATACTCCTGGCAACGCCTGCGGTGCGCAACCTGGTGC
GCGAGGGCAAAGTGGCGCAGATTTATTCGGCGATACAGACAGGCGGGGCACAGGGGATGCAGACCCTCGACGCGTGTCTG
CAGGGCCTGGTGCGTCAAGGGCTGGTCAGCCCTGAAAGCGCCCGGGAGCGGGCGCTGGGGCGCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

60.651

98.83

0.599
  pilT Pseudomonas stutzeri DSM 10701

60.355

98.83

0.596
  pilT Acinetobacter baumannii D1279779

59.701

97.953

0.585
  pilT Acinetobacter baumannii strain A118

59.701

97.953

0.585
  pilT Acinetobacter nosocomialis M2

59.701

97.953

0.585
  pilT Acinetobacter baylyi ADP1

58.806

97.953

0.576
  pilT Neisseria meningitidis 8013

56.637

99.123

0.561
  pilT Neisseria gonorrhoeae MS11

56.342

99.123

0.558
  pilT Legionella pneumophila strain ERS1305867

54.839

99.708

0.547
  pilT Legionella pneumophila strain Lp02

54.839

99.708

0.547
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.303

98.538

0.535
  pilT Vibrio cholerae strain A1552

54.303

98.538

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

99.415

0.497
  pilU Pseudomonas stutzeri DSM 10701

38.806

97.953

0.38
  pilU Acinetobacter baylyi ADP1

37.243

99.708

0.371