Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MRX62_RS12155 Genome accession   NZ_CP104027
Coordinates   2556309..2558009 (+) Length   566 a.a.
NCBI ID   WP_046419592.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. pauca strain 50088     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2551309..2563009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRX62_RS12140 (MRX62_12155) - 2551387..2552298 (+) 912 WP_060872176.1 AEC family transporter -
  MRX62_RS12145 (MRX62_12160) - 2552338..2552532 (+) 195 WP_010894016.1 hypothetical protein -
  MRX62_RS12150 (MRX62_12165) - 2553201..2556017 (+) 2817 WP_369210800.1 YadA-like family protein -
  MRX62_RS12155 (MRX62_12170) pilF 2556309..2558009 (+) 1701 WP_046419592.1 type II secretion system ATPase GspE Machinery gene
  MRX62_RS12160 (MRX62_12175) - 2558045..2559262 (+) 1218 WP_010894019.1 type II secretion system F family protein -
  MRX62_RS12165 (MRX62_12180) gspG 2559479..2559913 (+) 435 WP_004083686.1 type II secretion system major pseudopilin GspG -
  MRX62_RS12170 (MRX62_12185) - 2559919..2560425 (+) 507 WP_004083685.1 Tfp pilus assembly protein FimT/FimU -
  MRX62_RS12175 (MRX62_12190) - 2560422..2560820 (+) 399 WP_023906685.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MRX62_RS12180 (MRX62_12195) - 2560817..2561443 (+) 627 WP_023906684.1 type II secretion system protein J -
  MRX62_RS12185 (MRX62_12200) - 2561443..2562288 (+) 846 WP_023906683.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62589.37 Da        Isoelectric Point: 5.0845

>NTDB_id=626466 MRX62_RS12155 WP_046419592.1 2556309..2558009(+) (pilF) [Xylella fastidiosa subsp. pauca strain 50088]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPELSA
EVHGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGAALQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPADDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGVLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFIDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPTHAERYLASPEQVARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDALRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=626466 MRX62_RS12155 WP_046419592.1 2556309..2558009(+) (pilF) [Xylella fastidiosa subsp. pauca strain 50088]
ATGAATGTAAGTATGGAAGCATCCATCGTCGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGCGATCTGTATCGCGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGCTGGGTCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTGTCGGCC
GAGGTGCATGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGCCCGCTTGCTGAGCATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGTTGCGCCTTGCCACGGGTGCAGCATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAGGGCCGCAGCGTCATGGGCATGATTGTGGAGGCG
ACCGATGGTGATGCGGCGCCCGCAGATGATATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATCCAGCGTGCAGTTGAATTACGTGCCTCTGATATCCACATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCGTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAG
ATCATGGCCAAGCTTAATATTGCTGAACGTCGCTTGCCTCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGATCGTGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTATTGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTG
CTGGTCACTGGCCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATCGAAGGTATCAATCAGATCCAGGCTAAGCCGCAGATCGGGCTGG
ATTTTGCCAGTGCATTACGCAGCATTGTGCGTCAGGATCCAGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCCGCGCTGACAGGGCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTTGATATGGGTGTGGAAGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCGCAGCGTTTGGTGC
GTCGCTTGGAGCCTACGCATGCTGAGCGTTATCTTGCCTCGCCAGAGCAGGTTGCGCGTTTTGATTTGCGCCGTTTACAG
CCGGAGGGTGATATTTATTTGTATCGTCCGCGCGCTTCAGCGTTGGCACCCACTGGGTATCTGGGTCGTACGGCGATCGT
CGAATTTCTAATTATGGATGATGCGCTGCGCCGCGCGGTGATCAGCCGTACCGGCATAGGAGAGCTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACCACGATTGAAGAGGTCTTG
CGTGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(189-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.794

97.88

0.399
  pilB Vibrio campbellii strain DS40M4

40.516

95.936

0.389
  pilB Vibrio parahaemolyticus RIMD 2210633

40.257

96.113

0.387
  pilB Vibrio cholerae strain A1552

38.38

100

0.385
  pilB Legionella pneumophila strain ERS1305867

36.842

100

0.371