Detailed information NTG0626263

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LN920_RS10635
• Genome accession: NZ_CP086759
• Coordinates: 2219067..2220185 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain ACI713
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2214067..2225185

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LN920_RS10605 (LN920_10605)	-	2214896..2215744 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
LN920_RS10610 (LN920_10610)	-	2215997..2216452 (+)	456	WP_000782976.1	bacteriohemerythrin	-
LN920_RS10615 (LN920_10615)	-	2216605..2217672 (+)	1068	WP_002019572.1	hypothetical protein	-
LN920_RS10620 (LN920_10620)	-	2217672..2217995 (+)	324	WP_000442587.1	RnfH family protein	-
LN920_RS10625 (LN920_10625)	-	2218050..2218448 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
LN920_RS10630 (LN920_10630)	fur	2218560..2218997 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
LN920_RS10635 (LN920_10635)	pilU	2219067..2220185 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LN920_RS10640 (LN920_10640)	pilT	2220213..2221250 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
LN920_RS10645 (LN920_10645)	-	2221377..2222069 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=626263 LN920_RS10635 WP_000347039.1 2219067..2220185(-) (pilU) [Acinetobacter baumannii strain ACI713]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=626263 LN920_RS10635 WP_000347039.1 2219067..2220185(-) (pilU) [Acinetobacter baumannii strain ACI713]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395