Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NYR19_RS05040 Genome accession   NZ_CP103874
Coordinates   1067566..1068126 (-) Length   186 a.a.
NCBI ID   WP_039162437.1    Uniprot ID   -
Organism   Gallibacterium anatis strain TH22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1062566..1073126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR19_RS05020 (NYR19_05020) - 1063271..1063891 (-) 621 WP_039081190.1 tetratricopeptide repeat protein -
  NYR19_RS05025 (NYR19_05025) hisS 1063903..1065186 (-) 1284 WP_210640508.1 histidine--tRNA ligase -
  NYR19_RS05030 (NYR19_05030) ispG 1065208..1066323 (-) 1116 WP_210640509.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NYR19_RS05035 (NYR19_05035) - 1066348..1067421 (-) 1074 WP_210640510.1 RodZ domain-containing protein -
  NYR19_RS05040 (NYR19_05040) pilF 1067566..1068126 (-) 561 WP_039162437.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NYR19_RS05045 (NYR19_05045) - 1068147..1069265 (-) 1119 WP_013745260.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NYR19_RS05050 (NYR19_05050) folD 1069588..1070451 (-) 864 WP_210640511.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
  NYR19_RS05065 (NYR19_05065) - 1070927..1071205 (-) 279 WP_039154750.1 hypothetical protein -
  NYR19_RS05070 (NYR19_05070) - 1071202..1071381 (-) 180 WP_013745256.1 hypothetical protein -
  NYR19_RS05075 (NYR19_05075) udk 1071586..1072242 (+) 657 WP_013745255.1 uridine kinase -
  NYR19_RS05080 (NYR19_05080) dcd 1072258..1072839 (+) 582 WP_021462404.1 dCTP deaminase -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 21007.98 Da        Isoelectric Point: 9.4872

>NTDB_id=625939 NYR19_RS05040 WP_039162437.1 1067566..1068126(-) (pilF) [Gallibacterium anatis strain TH22]
MNKTMIKLKFFILLSSLFTLFACSHSVNNKPLSANAAAAKTRVELGLAYLSQGNPALAKANLDKALQHTPNDYLPYLGLA
YFYQHTQQLKLAGEYYQKALSLSPKNGDVLNNYATYLCSQRQFDTAFNFFEQALNSKAYYHFADTYENIAICAVLAGDQN
KTEQALTQLEKYSVDKAKNLRQRLHK

Nucleotide


Download         Length: 561 bp        

>NTDB_id=625939 NYR19_RS05040 WP_039162437.1 1067566..1068126(-) (pilF) [Gallibacterium anatis strain TH22]
ATGAACAAAACAATGATTAAACTGAAATTTTTTATTCTGCTTTCCTCACTGTTTACGCTTTTTGCCTGTAGTCATTCGGT
TAACAATAAACCGCTTTCCGCTAATGCTGCTGCGGCAAAAACGCGTGTTGAGTTAGGGTTGGCTTATTTGTCGCAAGGCA
ATCCGGCATTGGCAAAAGCCAATTTAGATAAAGCGTTGCAACATACACCGAATGATTATTTGCCTTATCTAGGTTTGGCT
TATTTTTATCAACATACCCAACAACTCAAACTTGCCGGCGAATATTATCAAAAAGCGCTCTCTCTTTCGCCCAAGAATGG
CGATGTATTGAATAATTATGCAACTTACCTCTGCTCTCAACGCCAATTTGATACGGCATTTAACTTTTTTGAACAAGCAT
TAAACAGCAAAGCCTATTATCATTTTGCCGATACTTATGAAAACATTGCGATTTGCGCTGTTTTAGCCGGTGATCAAAAC
AAAACTGAACAAGCTTTAACCCAACTGGAGAAATATTCTGTCGATAAAGCGAAAAATTTGCGGCAACGTTTGCATAAATA
A

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(40-68)

Tetratricopeptide repeat (TPR_17)

(96-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

45.902

98.387

0.452
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.988

89.247

0.419