Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LK336_RS02255 Genome accession   NZ_CP086572
Coordinates   433650..434720 (+) Length   356 a.a.
NCBI ID   WP_031787261.1    Uniprot ID   -
Organism   Staphylococcus argenteus strain RIVM_M036020     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428650..439720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK336_RS02225 (LK336_02225) - 429073..430536 (+) 1464 WP_031787264.1 rhomboid family intramembrane serine protease -
  LK336_RS02230 (LK336_02230) - 430517..430717 (+) 201 WP_000087563.1 YqgQ family protein -
  LK336_RS02235 (LK336_02235) - 430717..431703 (+) 987 WP_000161316.1 ROK family glucokinase -
  LK336_RS02240 (LK336_02240) - 431703..432032 (+) 330 WP_001018874.1 MTH1187 family thiamine-binding protein -
  LK336_RS02245 (LK336_02245) - 432029..432652 (+) 624 WP_031787263.1 MBL fold metallo-hydrolase -
  LK336_RS02250 (LK336_02250) comGA 432704..433678 (+) 975 WP_031787262.1 competence type IV pilus ATPase ComGA Machinery gene
  LK336_RS02255 (LK336_02255) comGB 433650..434720 (+) 1071 WP_031787261.1 competence type IV pilus assembly protein ComGB Machinery gene
  LK336_RS02260 (LK336_02260) comGC 434735..435046 (+) 312 WP_000921699.1 competence type IV pilus major pilin ComGC Machinery gene
  LK336_RS02265 (LK336_02265) comGD 435024..435470 (+) 447 WP_072324131.1 competence type IV pilus minor pilin ComGD Machinery gene
  LK336_RS02270 (LK336_02270) comGE 435457..435756 (+) 300 WP_031787259.1 hypothetical protein Machinery gene
  LK336_RS02275 (LK336_02275) comGF 435716..436171 (+) 456 WP_078097699.1 competence type IV pilus minor pilin ComGF Machinery gene
  LK336_RS02280 (LK336_02280) - 436270..436419 (+) 150 WP_072324192.1 hypothetical protein -
  LK336_RS02285 (LK336_02285) - 436406..436930 (+) 525 WP_031787256.1 shikimate kinase -
  LK336_RS02290 (LK336_02290) gcvT 437087..438178 (+) 1092 WP_000093342.1 glycine cleavage system aminomethyltransferase GcvT -
  LK336_RS02295 (LK336_02295) gcvPA 438197..439543 (+) 1347 WP_031787255.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41705.06 Da        Isoelectric Point: 10.2079

>NTDB_id=625528 LK336_RS02255 WP_031787261.1 433650..434720(+) (comGB) [Staphylococcus argenteus strain RIVM_M036020]
MKVQWISIFKKYSKKRQLNRAQQIELIVNLKNLLQYGFTLYQSFQFLNLQIKYKDKELSSKILSEISNGASCSKILAMLG
YSDTIVMQIYLAERFGNIVDILDETVKFMKINRKSEQRLLKTLQYPLVLVSVFIGMIMMLNITVIPQFQQLYTSMNIKLS
TFQKALSFFISSLPSLILITIFLILILTSSIKLIYNRLTMLYKINFMMKIPILSSYYKLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGEYLLTYSEKGHGLPEILSNLKCFKPQLIKFIQQGEKRGKLEVELRLYSQILVKQIEDKAIR
QTQFIQPILFLILGLFIVAIYLVIMLPMFQMMQSIN

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=625528 LK336_RS02255 WP_031787261.1 433650..434720(+) (comGB) [Staphylococcus argenteus strain RIVM_M036020]
GTGAAAGTACAATGGATAAGTATATTTAAAAAATATTCTAAGAAACGACAATTAAACAGAGCACAACAAATAGAGTTAAT
TGTAAATTTAAAGAATTTACTACAATATGGGTTCACATTATATCAAAGCTTTCAGTTTCTAAACCTTCAAATTAAATATA
AAGATAAAGAGCTATCGTCAAAAATTCTTAGCGAAATTTCAAATGGTGCATCGTGTAGCAAAATACTAGCCATGTTAGGC
TATAGTGATACTATAGTAATGCAAATTTACTTAGCAGAAAGGTTTGGTAATATTGTAGATATCTTAGATGAAACAGTGAA
ATTTATGAAAATAAATAGAAAATCCGAGCAAAGATTATTAAAAACTTTGCAATATCCCTTAGTGTTAGTTTCAGTATTTA
TAGGAATGATTATGATGTTGAATATCACTGTTATTCCACAATTCCAACAACTATATACTTCAATGAATATTAAATTATCA
ACATTTCAAAAAGCACTTTCCTTTTTTATCTCAAGTTTACCCTCATTAATTTTAATAACAATTTTTCTTATTTTAATTTT
AACCTCCTCAATAAAATTAATTTATAACAGGCTAACGATGTTATATAAAATCAACTTTATGATGAAGATACCAATTTTAT
CCAGTTATTATAAGTTGTTCAAAACTTATTTTGTTACAAATGAATTAGTTTTATTCTATAAAAATGGTATTACACTCCAA
TCTATCGTTGATGTTTATATCAATCATAGTAGTGATCCATTCAGACAATTTTTAGGTGAATATTTATTAACATATTCAGA
AAAAGGACATGGGTTACCGGAAATTTTATCAAATTTAAAGTGCTTTAAACCACAATTGATAAAGTTTATACAGCAAGGTG
AAAAAAGAGGGAAATTAGAAGTTGAGCTTAGATTATACTCTCAAATTTTAGTGAAACAAATTGAAGATAAAGCGATTCGT
CAAACTCAATTTATACAACCAATTTTATTTTTAATCTTAGGGCTTTTTATTGTTGCAATTTATTTGGTAATAATGCTACC
AATGTTCCAAATGATGCAAAGTATTAATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(32-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

78.028

99.719

0.778
  comGB Staphylococcus aureus N315

78.028

99.719

0.778