Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   N288_RS06725 Genome accession   NC_022524
Coordinates   1295833..1299324 (+) Length   1163 a.a.
NCBI ID   WP_009794014.1    Uniprot ID   -
Organism   Bacillus infantis NRRL B-14911     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1290833..1304324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N288_RS06705 (N288_07190) - 1291395..1291952 (-) 558 WP_009794010.1 cysteine hydrolase family protein -
  N288_RS06710 (N288_07195) - 1292093..1293601 (+) 1509 WP_009794011.1 class I adenylate-forming enzyme family protein -
  N288_RS06715 (N288_07200) - 1293675..1294223 (+) 549 WP_009794012.1 GNAT family N-acetyltransferase -
  N288_RS06720 (N288_07205) - 1294340..1295467 (-) 1128 WP_009794013.1 MFS transporter -
  N288_RS06725 (N288_07210) addB 1295833..1299324 (+) 3492 WP_009794014.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  N288_RS06730 (N288_07215) addA 1299317..1303081 (+) 3765 WP_009794015.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  N288_RS06735 (N288_07220) - 1303159..1303461 (+) 303 WP_009794016.1 HNH endonuclease -
  N288_RS06740 (N288_07225) - 1303506..1303724 (-) 219 WP_009794017.1 spore germination protein -
  N288_RS06745 (N288_07230) - 1303769..1304146 (-) 378 WP_009794018.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1163 a.a.        Molecular weight: 133284.74 Da        Isoelectric Point: 6.2957

>NTDB_id=62490 N288_RS06725 WP_009794014.1 1295833..1299324(+) (addB) [Bacillus infantis NRRL B-14911]
MSVRFILGRSGSGKTSLCLEEIREKMKIEPNGHPVVYLVPEQMTFLSEYKLITTPGLGGMIRTQVLSFTRLAWKVLQETG
GISRYHLNSTGVNMLIRKIIEDKKDELKIFQRAAGKNGFIGQMEQMLTEFKRYCIEPGALAGKREELESRRGSRGLQDKL
HDMELIYKQFEEGLLGKYVDSEDYFRLLTEKAGMSEYLKNAEVYIDGFYSYTPQEYMIIEQLIRVCKRVTVTFTLDRPYK
TAVPEDLHLFRMSAESYQSVYEIAGMAGAEMEQEVLLNGQQRWDNDSLRHMERAFSSRPAEPFAGKANVHIAQAVNRRAE
IEGAAREIKRLAAEEGYRFKDIALLMRNGSVYHDIIETIFDDYGIPFFIDQKRTMMNHPLIELIRSSLEVITGYWRYEPV
FRAVKTELLFPSRADLGVIREKMDKLENYVLAYGVQGEKWTKKDRWNYRRIRGLEFEQIVQTDLEKEREEELNQMRDLIA
APILRLSRRLKKADSGRRLCEAVYLYLEELDIPTKLERWKLEEEDKGNLVKAREHDQAWNAVMELLDQFVEMLGDEKISM
KQFASILDSGMESLRFSLVPPAMDQVLAADLDKSRLSDIKAAFVIGLNEGVLPGKFTEEGVLADADREKLLQSGLKIAHG
SKVRLLDEDFLAYRAFSTPSSHLYISYPLANEEGKALMPSPYISRANELFPEAGQLMWMADPFELPEEEQLLFAMNEDTS
LSYLASQLQMNKRGYKISGIWWDVYNYYHAHPRLGDTARKVLSSLHYENRTARLSEEAAKELYGETIQASVSRMELFHSC
PFSHFAQHGLKLRDRKVFRLEAPDIGDLFHAALKFIAETVMDKRMSWSDMTRQECEKLAKEAVEMLAPKLQNEILLSSNR
HFYIKRKLENIISRASYILSEHSRASGFSPVGLELGFGPKDKLPPLSFKLRNGTNMELVGRIDRVDKAENENGVYLRVID
YKSSARDLNISEVYYGLALQMLTYLDIIITHSGDIAGKKADPAGVMYFHVHNPIVNSPKLLTLDEIEEEIFKKFKMNGLL
LGDENVLRLMDQTLDSGDSSIISAGFKKDGTITKRSKVATRDDFESLRKFVRGTYTKTGDAIISGSADISPYKMKDKVPC
TFCSFKSVCQFDESVESNEYRVLSPKSNEELMDKIRKEAGENG

Nucleotide


Download         Length: 3492 bp        

>NTDB_id=62490 N288_RS06725 WP_009794014.1 1295833..1299324(+) (addB) [Bacillus infantis NRRL B-14911]
ATGTCTGTACGTTTTATATTAGGCCGGAGCGGCAGCGGCAAGACTTCGCTCTGCCTTGAAGAAATCAGGGAGAAAATGAA
AATCGAGCCGAATGGCCATCCAGTTGTTTATCTGGTGCCTGAGCAGATGACGTTTCTTTCGGAATATAAGCTCATCACAA
CCCCGGGGCTTGGCGGGATGATCCGTACACAGGTCTTGTCATTTACAAGGCTTGCCTGGAAGGTGCTGCAGGAAACAGGC
GGGATCAGCCGCTATCATTTGAACAGCACCGGCGTTAATATGCTCATCAGAAAAATAATTGAAGATAAAAAAGATGAACT
GAAAATCTTTCAGCGGGCGGCAGGCAAGAACGGCTTTATCGGCCAAATGGAGCAGATGCTGACAGAATTCAAACGGTACT
GCATTGAGCCGGGAGCTCTTGCAGGGAAACGGGAAGAACTTGAATCCCGAAGGGGCAGCAGGGGGCTTCAGGACAAGCTG
CATGATATGGAATTGATCTATAAGCAGTTCGAAGAAGGCCTTCTTGGCAAGTACGTAGATTCTGAAGACTATTTCCGCCT
TCTTACTGAAAAAGCAGGGATGTCGGAGTACCTGAAAAATGCCGAGGTGTATATCGACGGCTTTTACAGCTATACGCCCC
AGGAATATATGATCATCGAGCAGCTTATCAGAGTCTGCAAAAGAGTGACGGTGACATTTACGCTTGACAGGCCATATAAA
ACAGCAGTCCCGGAAGATCTTCATCTTTTCAGGATGTCGGCAGAATCATACCAGTCGGTCTATGAGATTGCGGGGATGGC
CGGGGCCGAGATGGAACAGGAAGTGCTTCTCAATGGTCAGCAGCGCTGGGACAATGACTCGCTCCGCCATATGGAAAGAG
CCTTCAGCAGCAGGCCTGCCGAGCCTTTTGCAGGAAAAGCAAACGTCCATATTGCACAGGCAGTGAACAGGAGAGCAGAA
ATAGAAGGTGCGGCCAGAGAGATTAAGAGACTGGCAGCAGAAGAGGGATACCGATTTAAAGACATTGCCCTTTTAATGAG
GAATGGATCAGTTTACCATGACATCATTGAAACCATCTTTGATGATTACGGGATTCCGTTTTTTATCGATCAAAAGCGGA
CGATGATGAACCATCCGCTTATTGAGCTTATCCGCTCATCACTTGAGGTCATTACCGGCTACTGGAGGTATGAGCCTGTT
TTTCGCGCAGTCAAAACCGAACTGCTGTTTCCGTCCCGGGCGGATCTTGGCGTGATCAGGGAAAAAATGGACAAGCTTGA
AAACTATGTTCTGGCATATGGTGTCCAGGGCGAGAAGTGGACAAAAAAGGACCGCTGGAATTACCGCCGCATCAGGGGGC
TTGAATTTGAGCAGATCGTCCAGACCGACTTGGAAAAAGAGCGGGAAGAAGAGCTGAATCAAATGAGGGACCTGATCGCT
GCACCGATACTCCGGCTCTCCAGGAGGCTTAAGAAGGCCGACAGCGGAAGAAGGCTGTGCGAGGCTGTCTATCTTTATTT
GGAGGAATTGGATATACCGACGAAGCTGGAGCGCTGGAAGCTGGAAGAGGAAGATAAGGGGAATCTTGTAAAGGCAAGGG
AACATGATCAGGCCTGGAATGCTGTCATGGAGCTTCTGGACCAGTTTGTAGAAATGCTCGGGGATGAGAAGATCAGCATG
AAGCAGTTTGCCTCAATCCTTGATTCAGGAATGGAATCTCTCCGCTTCTCTCTTGTTCCGCCGGCTATGGACCAGGTGCT
CGCAGCAGATCTGGACAAATCTCGTTTGTCGGATATAAAGGCAGCGTTTGTCATCGGGCTGAATGAGGGTGTCCTGCCTG
GCAAGTTCACTGAGGAAGGAGTGCTTGCTGATGCTGACCGGGAGAAGCTTCTGCAGTCAGGGCTTAAAATTGCCCATGGC
AGCAAGGTCAGGCTCCTGGATGAGGATTTTCTGGCTTATCGCGCCTTTTCCACACCTTCCTCCCATCTGTACATCAGCTA
TCCGCTCGCCAATGAAGAGGGCAAGGCACTGATGCCGTCGCCTTATATCAGCCGGGCAAACGAACTGTTCCCGGAGGCGG
GGCAGCTGATGTGGATGGCCGATCCGTTCGAACTTCCTGAGGAGGAGCAGCTATTATTCGCCATGAACGAGGATACGAGT
CTTTCTTATCTGGCTTCCCAGCTCCAGATGAATAAAAGAGGCTATAAAATCAGCGGAATCTGGTGGGATGTCTACAATTA
TTATCATGCCCATCCAAGGCTCGGGGACACGGCCAGAAAAGTACTTTCCAGCCTGCATTACGAAAACAGAACGGCCAGGC
TTTCGGAAGAGGCTGCCAAGGAGCTTTACGGGGAAACGATCCAGGCAAGTGTATCAAGGATGGAGCTTTTCCACAGCTGC
CCATTTTCACATTTTGCCCAGCATGGCCTGAAGCTCCGGGACAGGAAGGTCTTCCGCCTGGAAGCGCCGGATATCGGAGA
TCTGTTCCATGCTGCGTTGAAATTCATAGCTGAGACCGTCATGGATAAAAGGATGTCCTGGTCTGACATGACAAGGCAGG
AATGCGAGAAGCTTGCCAAAGAAGCGGTGGAGATGCTTGCGCCAAAGCTTCAGAATGAGATACTGCTGAGCTCGAACCGC
CACTTTTATATCAAGCGTAAGCTCGAAAATATCATCAGCAGGGCTTCGTATATACTGAGCGAGCACAGCAGGGCAAGCGG
GTTCTCCCCAGTCGGCCTTGAGCTCGGCTTTGGCCCTAAGGATAAACTTCCGCCTCTGTCCTTTAAGTTGAGGAATGGGA
CGAACATGGAGCTGGTCGGCCGGATTGATCGGGTTGATAAAGCGGAAAATGAAAATGGCGTATACCTTCGGGTGATCGAT
TATAAATCCAGCGCAAGGGATCTGAATATCAGTGAAGTCTACTATGGGCTTGCTCTGCAGATGCTTACCTACCTGGACAT
CATCATCACCCACTCAGGGGATATAGCAGGGAAAAAGGCCGATCCTGCAGGAGTCATGTACTTCCATGTCCATAATCCGA
TTGTGAACTCTCCGAAGCTTCTCACACTCGATGAGATTGAAGAGGAGATCTTTAAGAAGTTCAAAATGAACGGCCTTCTT
CTCGGCGATGAAAATGTTTTAAGGCTCATGGACCAGACACTTGATTCCGGCGACTCCTCGATTATATCGGCTGGATTTAA
AAAGGACGGCACCATCACGAAAAGGTCGAAGGTAGCCACAAGGGACGACTTCGAGAGCCTTCGGAAATTTGTCCGGGGAA
CCTACACAAAAACCGGGGACGCGATCATCAGCGGATCAGCAGATATTTCCCCTTATAAAATGAAGGACAAGGTGCCTTGT
ACATTCTGTTCGTTCAAGTCAGTCTGCCAGTTTGATGAATCGGTTGAATCGAATGAGTACAGGGTGCTTTCCCCTAAGTC
AAATGAAGAACTGATGGATAAGATACGAAAGGAGGCGGGAGAGAATGGCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(789-1130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

50.813

100

0.511

Multiple sequence alignment