Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N288_RS00590 Genome accession   NC_022524
Coordinates   111880..113262 (+) Length   460 a.a.
NCBI ID   WP_009791538.1    Uniprot ID   U5L456
Organism   Bacillus infantis NRRL B-14911     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 106880..118262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N288_RS00570 (N288_00585) - 107073..107537 (+) 465 WP_263623464.1 CtsR family transcriptional regulator -
  N288_RS00575 (N288_00590) - 107565..108113 (+) 549 WP_009791535.1 UvrB/UvrC motif-containing protein -
  N288_RS00580 (N288_00595) - 108118..109185 (+) 1068 WP_009791536.1 protein arginine kinase -
  N288_RS00585 (N288_00600) clpC 109202..111655 (+) 2454 WP_009791537.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  N288_RS00590 (N288_00605) radA 111880..113262 (+) 1383 WP_009791538.1 DNA repair protein RadA Machinery gene
  N288_RS00595 (N288_00610) disA 113266..114339 (+) 1074 WP_009791539.1 DNA integrity scanning diadenylate cyclase DisA -
  N288_RS00600 (N288_00615) - 114550..115632 (+) 1083 WP_009791540.1 PIN/TRAM domain-containing protein -
  N288_RS00605 (N288_00620) ispD 115654..116346 (+) 693 WP_009791541.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  N288_RS00610 (N288_00625) ispF 116365..116844 (+) 480 WP_009791542.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49883.32 Da        Isoelectric Point: 7.1320

>NTDB_id=62482 N288_RS00590 WP_009791538.1 111880..113262(+) (radA) [Bacillus infantis NRRL B-14911]
MAKRKTKFICQECGYESAKWMGKCPACGQWNTMVEETEKPAAGGRRGAFAHSAGVTLASKATPITSIETVNEPRIYTDLK
ELNRVLGGGVVRGSLVLIGGDPGIGKSTLLLQVSSQLARKDHSVLYISGEESMRQTKLRADRLGVVSDKLLVYAETNLEE
ISRTIENENPSFVIIDSIQTVFHPDITSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVL
YFEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLEEVENPSEIFLEERSQGASGSTVVASMEGTRPVLVEIQALISPT
SFGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDKPTQASDCIIGEVG
LTGEVRRVSRIEQRVQEAAKLGFERIILPAKNLGGWDSPPGVQLIGVNTVSEALKAALGG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=62482 N288_RS00590 WP_009791538.1 111880..113262(+) (radA) [Bacillus infantis NRRL B-14911]
ATGGCAAAAAGAAAGACCAAATTCATCTGCCAGGAATGCGGCTATGAGTCCGCCAAGTGGATGGGCAAATGTCCTGCCTG
CGGGCAGTGGAATACAATGGTGGAGGAAACGGAGAAGCCTGCAGCTGGAGGAAGAAGGGGTGCATTTGCCCATTCGGCAG
GCGTAACCCTTGCTTCTAAAGCGACGCCGATCACCTCAATTGAGACAGTCAATGAACCGCGGATCTATACAGACCTGAAG
GAGCTCAACCGCGTTCTGGGAGGCGGGGTGGTAAGGGGCTCGCTTGTTCTGATCGGGGGCGACCCCGGAATCGGGAAGTC
GACGCTCCTCCTGCAGGTATCTTCCCAGCTGGCCCGGAAGGATCATTCTGTTCTTTATATTTCCGGAGAGGAATCCATGA
GGCAGACAAAGCTCAGGGCTGACAGGCTTGGGGTTGTATCAGATAAGCTGCTCGTATATGCAGAAACGAATCTCGAGGAA
ATCAGCCGGACCATTGAAAATGAAAATCCCAGCTTTGTGATCATTGATTCAATCCAGACTGTTTTTCATCCGGATATCAC
CTCGGCCCCTGGAAGCGTCTCGCAGGTAAGGGAATGTACCGCGGAGCTGATGAGGATCGGAAAGACCAAAGGCATCGCGA
TCTTTATCGTGGGGCATGTCACGAAGGAAGGATCGATTGCCGGCCCGAGGCTGCTCGAGCATATGGTTGACACAGTCCTC
TACTTCGAGGGTGAAAGGCATCATACGTACCGCATTCTCAGGGCTGTTAAGAACCGTTTCGGATCAACCAATGAAATGGG
CATCTTTGAAATGAAGGAATTCGGCCTTGAAGAGGTGGAAAATCCATCGGAAATATTCCTTGAAGAGCGCTCCCAGGGGG
CATCCGGATCTACAGTTGTTGCCTCCATGGAAGGGACGAGGCCGGTGCTGGTTGAAATTCAGGCGCTCATTTCCCCGACG
AGCTTCGGGAATCCGAGGAGGATGGCAACCGGGATCGATCATAACCGCGTCCCGCTTTTAATGGCTGTGCTTGAAAAGCG
TGTCGGGATGCTTCTGCAAAATCAGGATGCTTATTTGAAGGTGGCAGGCGGCGTTAAGCTGGATGAACCTGCGATTGACC
TGGCAATTGCCGTCAGCATTGCCTCAAGCTTCAGGGATAAGCCAACACAGGCCTCTGATTGCATCATTGGAGAAGTGGGT
TTGACCGGCGAAGTCCGCCGTGTCTCCAGGATCGAACAGCGTGTCCAGGAAGCTGCTAAGCTTGGGTTTGAACGGATCAT
CCTTCCTGCTAAGAACCTTGGAGGCTGGGACTCTCCGCCGGGCGTGCAGCTAATCGGGGTTAATACAGTCAGCGAGGCCT
TAAAAGCGGCATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-224)

Subunit ChlI of Mg-chelatase (ChlI)

(366-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U5L456

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.826

100

0.778
  radA Streptococcus pneumoniae Rx1

61.978

98.913

0.613
  radA Streptococcus pneumoniae D39

61.978

98.913

0.613
  radA Streptococcus mitis SK321

61.978

98.913

0.613
  radA Streptococcus pneumoniae R6

61.978

98.913

0.613
  radA Streptococcus pneumoniae TIGR4

61.978

98.913

0.613
  radA Streptococcus mitis NCTC 12261

61.572

99.565

0.613

Multiple sequence alignment