Detailed information NTG0624657

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: LMT64_RS06110
• Genome accession: NZ_CP086380
• Coordinates: 1208245..1210875 (-)
• Length: 876 a.a.
• NCBI ID: WP_126350933.1
• Uniprot ID: A0A431W5Y3
• Organism: Deinococcus radiophilus strain ATCC 27603
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1203245..1215875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LMT64_RS06100 (LMT64_06100)	lon	1204332..1206830 (+)	2499	WP_126350931.1	endopeptidase La	-
LMT64_RS06105 (LMT64_06105)	pilT	1206979..1208199 (-)	1221	WP_126350932.1	type IV pilus twitching motility protein PilT	Machinery gene
LMT64_RS06110 (LMT64_06110)	pilB	1208245..1210875 (-)	2631	WP_126350933.1	type II/IV secretion system protein	Machinery gene
LMT64_RS06115 (LMT64_06115)	-	1210954..1211493 (-)	540	WP_126350934.1	YqeG family HAD IIIA-type phosphatase	-
LMT64_RS06120 (LMT64_06120)	-	1211490..1212236 (-)	747	WP_126350935.1	polyphenol oxidase family protein	-
LMT64_RS06125 (LMT64_06125)	-	1212338..1213153 (+)	816	WP_126350936.1	enoyl-ACP reductase	-
LMT64_RS06130 (LMT64_06130)	-	1213250..1213747 (+)	498	WP_126350937.1	hypothetical protein	-
LMT64_RS06135 (LMT64_06135)	-	1213868..1214932 (+)	1065	WP_170165888.1	M42 family metallopeptidase	-
LMT64_RS06140 (LMT64_06140)	-	1215152..1215394 (+)	243	WP_126350938.1	hypothetical protein	-

Sequence

Protein

• Length: 876 a.a.
• Molecular weight: 95948.46 Da
• Isoelectric Point: 4.7532

>NTDB_id=624657 LMT64_RS06110 WP_126350933.1 1208245..1210875(-) (pilB) [Deinococcus radiophilus strain ATCC 27603]
MTLSIADRKLGAVLLDGGYISDEDLQRMVVRHAEVGGRLADVMIDSGVVGEKRLALVLSESLGIPMVDLLTTEPEPEALA
TVPGQVALNAGALPFALEGDLLHVAMIEPLNTLTAEVLEDETGFDIKAYQAMRAALYWRLAEHYPELGLEAQRPDTAAPG
QMRLGEHLLGRGLVNQAQLDMALKFQERTGEPLGQILVGQGALSSEQLYRALADQQSLEFLPDLSGFSVPDHVLSALSRT
DAVRYGGLPISRSEEGYEVVSHDPRHFSDIQALFTEPVKFLMSTPHQVEEATARLYPAPEPERVKDELQSLYAQSGGRSK
SLTDVIMEMGFADSSDIEAARQQQRGGQRLEDTLIQSGKLSPKMLAQSLATQLGYEFIDPAKNPPDTNVSMMVPEATARR
YSVVPVRLEGESLVVAMKDPRNVFALDDLQLITGRDIIPAVMVEKEITRLIEKYFGSKDMAELNQRLLEESRERQKLQES
TAEVDTALDDNAVVRTVENLIREAVLQDVSDIHIEPTETDVLVRYRIDGSLRKQASLPKGSAQALLARIKIMGGLDIAER
RVPQDGRVRFRKGAIDIDLRLSTLPTVYGEKAVMRLLQKAENIPEVEQLGFSDHNFRRYLEVIDKPNGIFLVTGPTGSGK
SFTSFSTLKRIAKPEKNTTTIEDPVEYEIPGIMQSQVNNAAGMTFARALRAFLRQDPDIIFVGEIRDGETAKIATEAALT
GHLVLATLHTNDAAGAIVRLEEMGVELFNIGAAVIGVLAQRLVRRVCKDCSQPTAADPEVLRRLGLSDADIQGADLRRGA
GCPRCGGTGYKGRTGIHELMVIDDTIRRAINTGKTATDIKDIAIDEGEMWTLRKDGIQKALQGITTLEEVLAVTSS

Nucleotide

• Length: 2631 bp

>NTDB_id=624657 LMT64_RS06110 WP_126350933.1 1208245..1210875(-) (pilB) [Deinococcus radiophilus strain ATCC 27603]
ATGACGTTATCAATTGCAGACCGTAAACTCGGCGCCGTATTGCTGGACGGTGGCTATATCTCCGACGAGGACCTTCAGCG
CATGGTGGTGCGGCACGCCGAAGTGGGTGGACGGCTGGCCGATGTGATGATCGATTCCGGCGTGGTAGGCGAAAAGCGCC
TGGCACTCGTTCTGTCCGAGAGTCTGGGCATTCCCATGGTGGATTTGCTCACCACCGAGCCAGAGCCGGAAGCCCTGGCT
ACTGTGCCGGGTCAAGTGGCGCTGAATGCCGGTGCCCTGCCCTTCGCGCTGGAGGGGGACTTACTCCATGTGGCGATGAT
CGAGCCGCTCAACACCCTGACCGCTGAGGTGCTGGAAGACGAAACCGGTTTTGATATCAAGGCCTATCAGGCGATGCGGG
CGGCCCTGTACTGGCGCCTGGCTGAGCACTATCCCGAACTGGGTCTGGAGGCCCAGCGCCCTGATACGGCGGCTCCGGGG
CAGATGCGCCTGGGCGAACACCTGCTGGGACGCGGTCTGGTCAATCAGGCACAGCTGGACATGGCCCTGAAGTTCCAGGA
GCGGACAGGCGAGCCGCTGGGGCAGATCCTGGTCGGTCAGGGTGCCCTGAGTAGCGAGCAGCTGTACCGCGCCTTGGCCG
ATCAGCAGAGCCTGGAGTTTCTGCCTGATCTGAGTGGCTTTAGTGTGCCTGACCATGTGCTCAGCGCTCTTTCCCGCACC
GACGCCGTGCGGTACGGCGGCTTGCCGATCAGCCGCAGCGAGGAAGGGTACGAGGTCGTCTCGCACGACCCGCGTCACTT
CTCAGATATCCAGGCCCTGTTCACAGAACCGGTCAAATTCCTGATGAGCACTCCACACCAAGTGGAGGAGGCCACTGCCC
GGTTGTACCCGGCCCCTGAGCCAGAGCGTGTCAAAGACGAACTGCAGTCGCTCTACGCCCAGAGTGGGGGCCGCTCCAAA
TCACTGACCGACGTGATCATGGAGATGGGCTTTGCCGATTCCAGCGACATTGAAGCGGCCCGGCAGCAGCAGCGCGGGGG
CCAGCGCCTGGAAGATACCCTGATTCAGTCGGGCAAGCTCAGCCCTAAAATGCTGGCGCAGTCGCTGGCGACGCAGCTGG
GTTACGAGTTTATCGATCCTGCGAAGAACCCTCCTGACACCAACGTGTCCATGATGGTCCCAGAAGCCACTGCGCGGCGT
TACAGCGTCGTCCCGGTCCGTCTGGAAGGCGAGTCGCTGGTCGTCGCCATGAAGGACCCACGCAACGTGTTTGCGCTGGA
CGATCTGCAGCTGATCACGGGGCGCGACATCATCCCGGCCGTGATGGTCGAGAAGGAAATCACTCGTCTGATCGAGAAGT
ATTTCGGCTCCAAGGACATGGCCGAGCTGAATCAGCGCCTGCTGGAAGAAAGCCGTGAGCGCCAGAAGTTGCAGGAGTCT
ACAGCGGAGGTGGATACGGCACTGGACGACAATGCCGTAGTCCGCACCGTCGAGAACCTGATTCGTGAAGCCGTCTTGCA
GGACGTGAGCGATATTCACATCGAGCCCACCGAAACCGATGTGCTGGTCCGGTACCGCATTGACGGATCGCTGCGGAAAC
AGGCCAGCTTGCCCAAGGGCTCGGCGCAGGCGCTGCTGGCCCGCATCAAGATCATGGGTGGGCTGGATATCGCCGAGCGG
CGTGTGCCGCAGGACGGGCGTGTGCGTTTCCGCAAGGGCGCGATTGACATTGACCTGCGTCTGTCCACGCTGCCCACCGT
ATACGGCGAAAAGGCTGTGATGCGCTTGCTGCAAAAAGCCGAGAACATTCCGGAAGTGGAGCAGTTGGGCTTCTCGGACC
ATAACTTCCGCCGCTACCTGGAAGTCATCGATAAGCCTAACGGCATCTTTCTGGTGACGGGGCCGACAGGTTCGGGGAAA
TCGTTTACCAGTTTCTCGACCCTCAAGCGCATCGCCAAGCCAGAGAAGAACACCACCACCATTGAAGACCCGGTGGAATA
CGAGATTCCCGGCATCATGCAGAGCCAGGTGAACAACGCTGCCGGGATGACTTTTGCGCGGGCGCTGCGTGCATTTCTCC
GCCAGGACCCCGACATCATCTTCGTGGGGGAGATCCGTGACGGCGAAACTGCCAAGATCGCCACCGAAGCGGCGCTGACT
GGTCACCTCGTGCTGGCCACCCTACACACCAACGACGCTGCTGGAGCCATCGTGCGCCTGGAGGAAATGGGTGTAGAGCT
GTTTAACATCGGGGCCGCCGTGATCGGCGTGCTTGCTCAGCGCCTGGTGCGGCGGGTCTGCAAGGACTGTTCCCAGCCCA
CTGCGGCTGACCCTGAGGTGCTGCGCCGTCTGGGCCTCAGCGACGCCGACATTCAGGGTGCCGACCTCCGCCGAGGAGCA
GGTTGCCCACGTTGTGGCGGCACCGGCTACAAAGGCCGTACGGGTATCCACGAACTGATGGTCATTGACGACACCATTCG
CCGTGCGATCAACACCGGTAAGACCGCCACCGACATCAAGGACATTGCCATTGACGAAGGGGAGATGTGGACGCTGCGCA
AAGACGGCATTCAGAAGGCCCTGCAAGGCATCACCACCCTAGAAGAAGTCCTGGCCGTGACCAGCAGCTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (354-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (197-300)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-143)

• Type II/IV secretion system protein (T2SSE) (500-765)

3D structure

• AlphaFold DB: A0A431W5Y3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	62.851	100	0.639
pilF	Thermus thermophilus HB27	55.407	100	0.567