Detailed information NTG0624527

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LLE50_RS04270
• Genome accession: NZ_CP086369
• Coordinates: 941789..942634 (-)
• Length: 281 a.a.
• NCBI ID: WP_116586646.1
• Uniprot ID: -
• Organism: Pectobacterium versatile strain A73-S18-O15
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 936789..947634

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLE50_RS04245 (LLE50_04245)	-	936968..937738 (-)	771	WP_107333187.1	ABC transporter ATP-binding protein	-
LLE50_RS04250 (LLE50_04250)	-	937740..938777 (-)	1038	WP_039492234.1	FecCD family ABC transporter permease	-
LLE50_RS04255 (LLE50_04255)	-	938774..939835 (-)	1062	WP_130621380.1	ABC transporter substrate-binding protein	-
LLE50_RS04260 (LLE50_04260)	-	940187..941212 (+)	1026	WP_103862229.1	LLM class flavin-dependent oxidoreductase	-
LLE50_RS04265 (LLE50_04265)	ypdK	941569..941643 (+)	75	WP_099606229.1	membrane protein YpdK	-
LLE50_RS04270 (LLE50_04270)	pilD	941789..942634 (-)	846	WP_116586646.1	prepilin peptidase	Machinery gene
LLE50_RS04275 (LLE50_04275)	-	942724..943470 (-)	747	WP_130621381.1	type II secretion system protein N	-
LLE50_RS04280 (LLE50_04280)	gspM	943460..943954 (-)	495	WP_103940960.1	type II secretion system protein GspM	-
LLE50_RS04285 (LLE50_04285)	gspL	943951..945228 (-)	1278	WP_127031378.1	type II secretion system protein GspL	-
LLE50_RS04290 (LLE50_04290)	gspK	945225..946211 (-)	987	WP_010302966.1	type II secretion system minor pseudopilin GspK	-
LLE50_RS04295 (LLE50_04295)	gspJ	946208..946858 (-)	651	WP_103940956.1	type II secretion system minor pseudopilin GspJ	-
LLE50_RS04300 (LLE50_04300)	gspI	946858..947223 (-)	366	WP_044208462.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31263.14 Da
• Isoelectric Point: 7.0813

>NTDB_id=624527 LLE50_RS04270 WP_116586646.1 941789..942634(-) (pilD) [Pectobacterium versatile strain A73-S18-O15]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGVADSDRDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSAQYPLVEVITMLAFLAAGLLWLPGMALLGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSGTFVSLNDAVVGAMAGYLSLWLLYWAFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=624527 LLE50_RS04270 WP_116586646.1 941789..942634(-) (pilD) [Pectobacterium versatile strain A73-S18-O15]
GTGGACGATTTAAGGGAATTCGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCGTGGCCGATTCCGATCGTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCTTGCCCGCACTGCCAACAGGCTATT
GCGGTCAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGTCATCAATCGGTCTCTGC
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGGCGGCAGGTTTGCTCTGGTTGCCCGGTATGGCGCTGT
TGGGGGCGTTGATCCTGTTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGTTGCTGTTTAATCTGTCGGGGACATTTGTTTCATTGAACGATGCCGTAGTCGGCGC
GATGGCTGGTTATTTATCTCTGTGGCTACTTTATTGGGCATTCAAATACGCCACGGGCAAAGAAGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAATCTGGTTTTGGTGGCAGCGTTGAGC
GGTCTGGTCGTTACTCTCATCTGGCGCGGGTTGCGTAAAGAGGATACGGCTAAACCGTTAGCTTTTGGCCCCTGGCTGGC
AATGGGTGGCGTGTTTGGTGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.37	96.085	0.484
pilD	Vibrio campbellii strain DS40M4	45.926	96.085	0.441
pilD	Neisseria gonorrhoeae MS11	48.627	90.747	0.441
pilD	Acinetobacter baumannii D1279779	43.223	97.153	0.42
pilD	Acinetobacter nosocomialis M2	42.857	97.153	0.416