Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGA   Type   Machinery gene
Locus tag   LL975_RS05565 Genome accession   NZ_CP086198
Coordinates   1067463..1068434 (+) Length   323 a.a.
NCBI ID   WP_236365614.1    Uniprot ID   -
Organism   Macrococcoides canis strain KW0150     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1031176..1070853 1067463..1068434 within 0


Gene organization within MGE regions


Location: 1031176..1070853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL975_RS05360 (LL975_05345) - 1031179..1031523 (+) 345 WP_226997687.1 diacylglycerol kinase family protein -
  LL975_RS05365 (LL975_05350) cdd 1031536..1031940 (+) 405 WP_086042767.1 cytidine deaminase -
  LL975_RS05370 (LL975_05355) era 1031940..1032842 (+) 903 WP_086042766.1 GTPase Era -
  LL975_RS05375 (LL975_05360) recO 1032842..1033591 (+) 750 WP_086042765.1 DNA repair protein RecO -
  LL975_RS05380 (LL975_05365) kynA 1033660..1034502 (-) 843 WP_236365630.1 tryptophan 2,3-dioxygenase -
  LL975_RS05385 (LL975_05370) kynB 1034486..1035118 (-) 633 WP_254254558.1 arylformamidase -
  LL975_RS05390 (LL975_05375) kynU 1035118..1036386 (-) 1269 WP_254254557.1 kynureninase -
  LL975_RS05395 (LL975_05380) - 1036655..1038043 (-) 1389 WP_254254556.1 glycine--tRNA ligase -
  LL975_RS05400 (LL975_05385) - 1038319..1038960 (+) 642 WP_086043666.1 helix-turn-helix transcriptional regulator -
  LL975_RS05405 (LL975_05390) - 1038972..1039787 (+) 816 WP_086042760.1 pyruvate, water dikinase regulatory protein -
  LL975_RS05410 (LL975_05395) dnaG 1039792..1041552 (+) 1761 WP_269886956.1 DNA primase -
  LL975_RS05415 (LL975_05400) rpoD 1041574..1042674 (+) 1101 WP_086042758.1 RNA polymerase sigma factor RpoD -
  LL975_RS05420 (LL975_05405) - 1042764..1043114 (+) 351 WP_254254553.1 c-type cytochrome -
  LL975_RS05425 (LL975_05410) - 1043188..1043877 (+) 690 WP_254254552.1 tRNA (adenine(22)-N(1))-methyltransferase -
  LL975_RS05430 (LL975_05415) - 1043864..1044946 (+) 1083 WP_269886959.1 Nif3-like dinuclear metal center hexameric protein -
  LL975_RS05435 (LL975_05420) - 1044982..1045950 (-) 969 WP_086042754.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  LL975_RS05440 (LL975_05425) - 1046081..1047409 (+) 1329 WP_086042753.1 DEAD/DEAH box helicase -
  LL975_RS05445 (LL975_05430) - 1047420..1048316 (+) 897 WP_254255410.1 deoxyribonuclease IV -
  LL975_RS05450 (LL975_05435) - 1048385..1049155 (+) 771 WP_269886961.1 metal ABC transporter ATP-binding protein -
  LL975_RS05455 (LL975_05440) - 1049158..1050003 (+) 846 WP_133419344.1 metal ABC transporter permease -
  LL975_RS05460 (LL975_05445) - 1049993..1050400 (+) 408 WP_086042750.1 Fur family transcriptional regulator -
  LL975_RS05465 (LL975_05450) - 1050425..1051003 (-) 579 WP_269886962.1 5' nucleotidase, NT5C type -
  LL975_RS05470 (LL975_05455) ispG 1050990..1052096 (-) 1107 WP_254255409.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LL975_RS05475 (LL975_05460) - 1052182..1052913 (-) 732 WP_254254550.1 DUF1189 family protein -
  LL975_RS05480 (LL975_05465) - 1053046..1053645 (+) 600 WP_086042747.1 superoxide dismutase -
  LL975_RS05485 (LL975_05470) - 1053799..1055808 (+) 2010 WP_254255408.1 peptidoglycan D,D-transpeptidase FtsI family protein -
  LL975_RS05490 (LL975_05475) - 1055950..1056912 (+) 963 WP_236365619.1 PstS family phosphate ABC transporter substrate-binding protein -
  LL975_RS05495 (LL975_05480) pstC 1056982..1057911 (+) 930 WP_086042744.1 phosphate ABC transporter permease subunit PstC -
  LL975_RS05500 (LL975_05485) pstA 1057913..1058806 (+) 894 WP_086042743.1 phosphate ABC transporter permease PstA -
  LL975_RS05505 (LL975_05490) pstB 1058831..1059721 (+) 891 WP_086042742.1 phosphate ABC transporter ATP-binding protein PstB -
  LL975_RS05510 (LL975_05495) phoU 1059714..1060352 (+) 639 WP_226997618.1 phosphate signaling complex protein PhoU -
  LL975_RS05515 (LL975_05500) rpmG 1060441..1060590 (+) 150 WP_041636027.1 50S ribosomal protein L33 -
  LL975_RS05520 (LL975_05505) ltrA 1061134..1062420 (+) 1287 WP_254254838.1 group II intron reverse transcriptase/maturase -
  LL975_RS05525 (LL975_05510) - 1062549..1063103 (+) 555 WP_157891076.1 5-formyltetrahydrofolate cyclo-ligase -
  LL975_RS05530 (LL975_05515) - 1063079..1064527 (+) 1449 WP_086042739.1 rhomboid family protein -
  LL975_RS05535 (LL975_05520) - 1064532..1064723 (+) 192 WP_086042738.1 YqgQ family protein -
  LL975_RS05540 (LL975_05525) - 1064720..1065688 (+) 969 WP_236365616.1 ROK family glucokinase -
  LL975_RS05545 (LL975_05530) - 1065688..1065999 (+) 312 WP_086042736.1 MTH1187 family thiamine-binding protein -
  LL975_RS05550 (LL975_05535) - 1066026..1066265 (-) 240 WP_174804788.1 DUF2759 domain-containing protein -
  LL975_RS05555 (LL975_05540) - 1066355..1066975 (+) 621 WP_086042734.1 MBL fold metallo-hydrolase -
  LL975_RS05560 (LL975_05545) - 1067003..1067251 (-) 249 WP_086042733.1 DUF2626 domain-containing protein -
  LL975_RS05565 (LL975_05550) comGA 1067463..1068434 (+) 972 WP_236365614.1 competence type IV pilus ATPase ComGA Machinery gene
  LL975_RS05570 (LL975_05555) comGB 1068409..1069458 (+) 1050 WP_254254547.1 competence type IV pilus assembly protein ComGB -
  LL975_RS05575 (LL975_05560) - 1069471..1069551 (+) 81 WP_269887095.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LL975_RS05580 (LL975_05565) - 1069639..1070853 (-) 1215 WP_086042729.1 tyrosine-type recombinase/integrase -

Sequence


Protein


Download         Length: 323 a.a.        Molecular weight: 37271.88 Da        Isoelectric Point: 6.7240

>NTDB_id=623599 LL975_RS05565 WP_236365614.1 1067463..1068434(+) (comGA) [Macrococcoides canis strain KW0150]
MEKLFNEIIEEAIIQGASDIHFIPNDKSISIKFRIHGDIEDYREIDDMLFRKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
KDLRYNIRASTLPRALGDEACVLRIIRQNFIDEYQTDDKILFNQIKKSSGIIIISGPTGSGKSTLMYQLVHYARNTLKRQ
IISIEDPVEQHIEGIIQVNVNEKAEITYHTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINTVDLYQSINLIINQRLVRKKDGKGRLLTYEFLTKKDIEKYLQGEHINYRTLTDQFKELYETNQISHYEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=623599 LL975_RS05565 WP_236365614.1 1067463..1068434(+) (comGA) [Macrococcoides canis strain KW0150]
ATGGAAAAATTATTCAATGAAATAATAGAGGAAGCAATTATACAAGGTGCATCAGATATTCACTTCATACCAAACGATAA
AAGCATATCTATCAAATTTAGGATACATGGTGATATTGAAGACTATAGAGAAATCGACGACATGTTATTCAGGAAATTAC
TTTCTTATATTAAATTTACGGCACATCTTGACGTATCAGAAAAGAATAAGGCACAGAGTGGAATCATCCATTTTAATCTG
AAAGACTTAAGATATAATATTCGCGCATCTACTTTACCTCGAGCATTAGGCGATGAAGCATGTGTACTGAGAATCATCAG
ACAAAATTTTATTGATGAATATCAGACGGATGACAAGATATTGTTCAATCAAATAAAAAAATCAAGCGGTATTATCATCA
TAAGTGGACCAACTGGAAGCGGGAAAAGCACATTGATGTACCAGCTTGTACATTATGCGAGGAACACATTGAAGAGACAA
ATAATATCAATAGAAGATCCTGTTGAACAGCATATTGAAGGAATTATACAAGTTAATGTTAACGAAAAAGCAGAGATAAC
ATATCATACCGCGATAAAGGCAATCCTTAGATGTGATCCGGATATCATTATGCTAGGTGAAATCAGAGATTCAGTCGTAG
CACAGCAAGTGATCAATGCAGGACTAAGCGGTCATCTCGTTTTAACGACATTACATGCGAATGATTGCATCGGTGCATTA
TTTCGGTTAAAAGAAATGGGTATTAATACTGTTGATCTATATCAAAGTATCAATCTGATTATTAATCAAAGGCTTGTAAG
AAAGAAAGATGGGAAGGGGCGTCTATTGACCTATGAATTTTTGACAAAAAAGGATATTGAAAAATATTTACAAGGTGAAC
ATATTAACTATAGAACGTTGACCGATCAATTTAAGGAGCTCTATGAAACAAATCAGATTTCACACTATGAATTCGAAAAA
TTTGATCTATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

48.78

100

0.495

  comGA Staphylococcus aureus N315

48.78

100

0.495

  comGA Bacillus subtilis subsp. subtilis str. 168

38.037

100

0.384

  pilB Glaesserella parasuis strain SC1401

34.012

100

0.362

  pilB Vibrio parahaemolyticus RIMD 2210633

34.513

100

0.362