Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   HIFGL_RS08425 Genome accession   NC_022356
Coordinates   1758034..1758573 (+) Length   179 a.a.
NCBI ID   WP_005649140.1    Uniprot ID   -
Organism   Haemophilus influenzae KR494     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1753034..1763573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIFGL_RS08405 (HifGL_001691) - 1753495..1754415 (-) 921 WP_005669098.1 ATP-binding cassette domain-containing protein -
  HIFGL_RS08410 (HifGL_001692) - 1754415..1755296 (-) 882 WP_005669100.1 metal ABC transporter substrate-binding protein -
  HIFGL_RS08415 (HifGL_001693) - 1755658..1756536 (-) 879 WP_005669102.1 D-alanyl-D-alanine carboxypeptidase family protein -
  HIFGL_RS08420 (HifGL_001695) - 1756783..1757934 (+) 1152 WP_042599461.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HIFGL_RS08425 (HifGL_001696) pilF/pilF2 1758034..1758573 (+) 540 WP_005649140.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HIFGL_RS08430 (HifGL_001697) - 1758645..1759556 (+) 912 WP_021035646.1 RodZ domain-containing protein -
  HIFGL_RS08435 (HifGL_001698) ispG 1759565..1760671 (+) 1107 WP_021035647.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  HIFGL_RS08440 (HifGL_001699) hisS 1760681..1761952 (+) 1272 WP_021035648.1 histidine--tRNA ligase -
  HIFGL_RS08445 (HifGL_001700) - 1761970..1762584 (+) 615 WP_005691752.1 YfgM family protein -
  HIFGL_RS08450 (HifGL_001701) iscX 1762635..1762829 (-) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  HIFGL_RS08455 (HifGL_001702) fdx 1762829..1763170 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20555.22 Da        Isoelectric Point: 8.6194

>NTDB_id=62175 HIFGL_RS08425 WP_005649140.1 1758034..1758573(+) (pilF/pilF2) [Haemophilus influenzae KR494]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=62175 HIFGL_RS08425 WP_005649140.1 1758034..1758573(+) (pilF/pilF2) [Haemophilus influenzae KR494]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.648

100

0.966
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458

Multiple sequence alignment