Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   LK420_RS00020 Genome accession   NZ_CP085949
Coordinates   2709..3248 (-) Length   179 a.a.
NCBI ID   WP_005649140.1    Uniprot ID   -
Organism   Haemophilus influenzae strain FDAARGOS_1562     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..8248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK420_RS00010 (LK420_00010) ispG 611..1717 (-) 1107 WP_021035647.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LK420_RS00015 (LK420_00015) - 1726..2637 (-) 912 WP_021035646.1 RodZ domain-containing protein -
  LK420_RS00020 (LK420_00020) pilF/pilF2 2709..3248 (-) 540 WP_005649140.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LK420_RS00025 (LK420_00025) - 3348..4499 (-) 1152 WP_042599461.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  LK420_RS00030 (LK420_00030) - 4746..5624 (+) 879 WP_005669102.1 D-alanyl-D-alanine carboxypeptidase family protein -
  LK420_RS00035 (LK420_00035) - 5986..6867 (+) 882 WP_005669100.1 metal ABC transporter substrate-binding protein -
  LK420_RS00040 (LK420_00040) - 6867..7787 (+) 921 WP_005669098.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20555.22 Da        Isoelectric Point: 8.6194

>NTDB_id=621497 LK420_RS00020 WP_005649140.1 2709..3248(-) (pilF/pilF2) [Haemophilus influenzae strain FDAARGOS_1562]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=621497 LK420_RS00020 WP_005649140.1 2709..3248(-) (pilF/pilF2) [Haemophilus influenzae strain FDAARGOS_1562]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.648

100

0.966
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458