Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   LIS66_RS22860 Genome accession   NZ_CP085885
Coordinates   5160939..5162528 (+) Length   529 a.a.
NCBI ID   WP_042610490.1    Uniprot ID   -
Organism   Pseudomonas sp. HN2     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5155939..5167528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS66_RS22840 (LIS66_22840) pgeF 5157740..5158462 (-) 723 WP_227973090.1 peptidoglycan editing factor PgeF -
  LIS66_RS22845 (LIS66_22845) rluD 5158459..5159421 (-) 963 WP_042610487.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  LIS66_RS22850 (LIS66_22850) - 5159573..5160589 (+) 1017 WP_064364119.1 outer membrane protein assembly factor BamD -
  LIS66_RS22855 (LIS66_22855) - 5160716..5160949 (+) 234 WP_039761661.1 PP0621 family protein -
  LIS66_RS22860 (LIS66_22860) pilS 5160939..5162528 (+) 1590 WP_042610490.1 sensor histidine kinase Regulator
  LIS66_RS22865 (LIS66_22865) pilR 5162525..5163871 (+) 1347 WP_227973091.1 sigma-54-dependent transcriptional regulator Regulator
  LIS66_RS22870 (LIS66_22870) thiO 5163965..5165065 (-) 1101 WP_227973092.1 glycine oxidase ThiO -
  LIS66_RS22875 (LIS66_22875) - 5165123..5165524 (-) 402 WP_227973093.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58639.25 Da        Isoelectric Point: 6.9043

>NTDB_id=621135 LIS66_RS22860 WP_042610490.1 5160939..5162528(+) (pilS) [Pseudomonas sp. HN2]
MVAEAANADQKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDDLLRGGSWLYLIINMLLVVFLENTRRPAQLF
TLALVDVLLLCGLFYAAGGVASALGNLLVVSVAISNTLLRRRIGLLIAAIAAIGIVGLSFLLSFSHPLSANAYLQAGTLG
ALCFAASLLVQGLIRRLEVSETLAEQRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSAQSLLGHSHLQGHLID
EYSPALVDRLQLWRNNPTLRPQSLKIPGNGLELQPSFIALEQSPNQQTLVFLEDLAQITQQAQQLKLAALGRLTAGISHE
IRNPLGAISHAAQLLAESEELDSADRRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLKPWLEDFVGETREQATER
QHIHLRINSGDFTTLMDPNQLRQILDNLIRNAWRHSAQLHEQAEVWLALFVDPDSQLAVLEVQDNGPGVSADEQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=621135 LIS66_RS22860 WP_042610490.1 5160939..5162528(+) (pilS) [Pseudomonas sp. HN2]
GTGGTCGCTGAGGCCGCCAACGCCGACCAGAAACAGGCTCAGCGTCTGCTGCGCCTTTATCACCTGTACCGCTTGAGTGT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACATGGACAACCGCCTGCTGACGTCGGCCAACGACGATCTGCTGCGCG
GTGGCAGCTGGCTGTATCTGATCATCAATATGCTGCTGGTGGTGTTCCTCGAAAACACCCGGCGCCCGGCGCAACTGTTC
ACTTTAGCGTTGGTCGATGTGCTGCTGTTGTGCGGCCTGTTCTACGCCGCTGGTGGCGTCGCCAGTGCGCTGGGCAATCT
GTTGGTCGTCTCGGTGGCGATCAGCAATACCCTGCTGCGCCGGCGCATCGGCCTGCTGATTGCCGCCATCGCGGCGATCG
GCATTGTCGGCCTGAGCTTTCTGCTGAGCTTCAGCCATCCCCTCAGCGCCAACGCCTACCTGCAAGCCGGCACCCTCGGA
GCGCTGTGCTTTGCCGCCTCGTTGCTGGTGCAAGGGCTGATCCGCCGCCTCGAAGTCAGTGAAACCCTCGCCGAACAGCG
CGCCAGTGAAGTGGTTGGCCTGGAAGCGCTCAACGCGTTGATCCTGCAACGCATGCGCACTGGCATTCTGGTGCTCGATG
AGCAACGCCGGGTGCAACTGGCCAATCACAGCGCGCAAAGCCTGCTCGGCCATAGCCACCTGCAAGGTCATTTGATCGAT
GAGTATTCGCCCGCGCTGGTCGATCGCCTGCAACTGTGGCGCAACAACCCGACCTTGCGCCCGCAGAGCCTGAAAATCCC
CGGCAACGGCCTCGAGCTGCAACCAAGCTTCATCGCCCTCGAGCAGAGCCCGAACCAGCAGACCCTGGTGTTTCTCGAAG
ATCTGGCGCAAATCACCCAACAGGCGCAGCAACTCAAACTCGCTGCGCTCGGGCGTTTGACCGCGGGCATCTCCCACGAA
ATTCGCAATCCGTTGGGCGCAATCAGTCATGCCGCGCAGCTGTTGGCCGAGTCCGAGGAACTCGATAGCGCGGATCGGCG
TCTGACGCAGATTATTCAAGATCACTCCCAGCGCATGAACCGAGTCATCGAAAACGTCCTGCAATTGTCCCGCCGTCAGC
AAAGCGCACCGCAACGGCTGGACCTCAAGCCGTGGCTGGAAGACTTCGTCGGCGAAACCCGCGAGCAAGCCACCGAGCGC
CAGCACATTCATCTACGGATCAATTCGGGCGACTTCACCACGCTGATGGACCCCAACCAGCTGCGGCAGATTCTCGACAA
TCTGATCCGCAACGCCTGGCGGCACAGTGCCCAGTTGCACGAGCAGGCCGAGGTCTGGCTGGCGCTGTTCGTCGACCCCG
ACAGCCAGTTGGCAGTACTCGAAGTGCAGGACAACGGCCCCGGCGTGTCGGCCGACGAGCAGGCGCATCTGTTCGAACCG
TTCTTCACCACCAGCAGCCAGGGCACCGGTCTTGGCCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCACGCCT
AGACTTCAAATCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-521)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.811

100

0.599