Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   GDK20_RS08140 Genome accession   NZ_AP019545
Coordinates   1656598..1657044 (-) Length   148 a.a.
NCBI ID   WP_001788899.1    Uniprot ID   -
Organism   Staphylococcus aureus strain KG-22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1651598..1662044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GDK20_RS08100 (SAKG22_15190) gcvT 1652558..1653649 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  GDK20_RS08105 (SAKG22_15200) - 1653808..1654332 (-) 525 WP_001015120.1 shikimate kinase -
  GDK20_RS08110 (SAKG22_15210) - 1654322..1654468 (-) 147 WP_001792109.1 hypothetical protein -
  GDK20_RS08115 (SAKG22_15220) - 1654565..1654675 (-) 111 Protein_1567 type IV pilus minor pilin ComGF family protein -
  GDK20_RS08125 (SAKG22_15230) - 1654774..1655946 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  GDK20_RS08130 (SAKG22_15240) - 1655996..1656394 (-) 399 Protein_1569 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GDK20_RS08135 (SAKG22_15250) comGE 1656312..1656611 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  GDK20_RS08140 (SAKG22_15260) comGD 1656598..1657044 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  GDK20_RS08145 (SAKG22_15270) comGC 1657022..1657333 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  GDK20_RS08150 (SAKG22_15280) comGB 1657347..1658417 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  GDK20_RS08155 (SAKG22_15290) comGA 1658389..1659363 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  GDK20_RS08160 (SAKG22_15300) - 1659415..1660038 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  GDK20_RS08165 (SAKG22_15310) - 1660035..1660364 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  GDK20_RS08170 (SAKG22_15320) - 1660364..1661350 (-) 987 WP_000161311.1 ROK family glucokinase -
  GDK20_RS08175 (SAKG22_15330) - 1661347..1661550 (-) 204 WP_000087561.1 YqgQ family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17165.33 Da        Isoelectric Point: 10.3312

>NTDB_id=61978 GDK20_RS08140 WP_001788899.1 1656598..1657044(-) (comGD) [Staphylococcus aureus strain KG-22]
MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVIDDEANIISFITELNYIKSQAIANQGYINVRFYENSD
TIKVIENNKIRFLKLKVGKIINVAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL

Nucleotide


Download         Length: 447 bp        

>NTDB_id=61978 GDK20_RS08140 WP_001788899.1 1656598..1657044(-) (comGD) [Staphylococcus aureus strain KG-22]
ATGGAGAAGCAGTTGCAAATTAGAAAGCAGTCAGCATTTACTATGATTGAGATGCTTGTGGTAATGATGTTAATCAGTAT
ATTTCTACTTTTGACAATGACATCTAAAGGATTAAGCAATCTTAGAGTAATAGATGATGAGGCAAATATCATTTCTTTTA
TTACTGAATTGAATTATATTAAGTCGCAAGCTATAGCAAATCAAGGATATATCAATGTTAGATTTTATGAAAACAGTGAC
ACTATTAAAGTAATAGAGAATAATAAAATACGATTTCTAAAATTAAAAGTAGGCAAAATAATTAATGTTGCAAAAGTTGA
TATTATTGCCTTTGATAAAAAAGGGAATATCAATAAATTTGGTAGCATAACAATTGACAATAACAATTCAATTTATAGAA
TAATATTCCATATTGAAAAAGGAAGAATTCGTTATGAAAAGCTATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Staphylococcus aureus MW2

100

100

1
  comGD Staphylococcus aureus N315

100

100

1

Multiple sequence alignment