Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   M892_RS12420 Genome accession   NC_022269
Coordinates   2689030..2690046 (+) Length   338 a.a.
NCBI ID   WP_041853270.1    Uniprot ID   -
Organism   Vibrio campbellii ATCC BAA-1116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2684030..2695046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M892_RS12405 (M892_12445) - 2684394..2685122 (-) 729 WP_012126438.1 redoxin family protein -
  M892_RS12410 (M892_12450) oxyR 2685297..2686205 (+) 909 WP_012126437.1 DNA-binding transcriptional regulator OxyR -
  M892_RS12415 (M892_12455) - 2686284..2688851 (-) 2568 WP_012126436.1 PBP1A family penicillin-binding protein -
  M892_RS12420 (M892_12460) pilM 2689030..2690046 (+) 1017 WP_041853270.1 type IV pilus assembly protein PilM Machinery gene
  M892_RS12425 (M892_12465) pilN 2690030..2690605 (+) 576 WP_012126434.1 PilN domain-containing protein Machinery gene
  M892_RS12430 (M892_12470) pilO 2690598..2691191 (+) 594 WP_012126433.1 type 4a pilus biogenesis protein PilO Machinery gene
  M892_RS12435 (M892_12475) pilP 2691181..2691696 (+) 516 WP_012126432.1 pilus assembly protein PilP Machinery gene
  M892_RS12440 (M892_12480) pilQ 2691731..2693479 (+) 1749 WP_041853271.1 type IV pilus secretin PilQ family protein Machinery gene
  M892_RS12445 (M892_12485) aroK 2693668..2694186 (+) 519 WP_010443904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37883.33 Da        Isoelectric Point: 6.4195

>NTDB_id=61962 M892_RS12420 WP_041853270.1 2689030..2690046(+) (pilM) [Vibrio campbellii ATCC BAA-1116]
MDKLLVTGIDIGHHSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=61962 M892_RS12420 WP_041853270.1 2689030..2690046(+) (pilM) [Vibrio campbellii ATCC BAA-1116]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATCACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGGGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAATCTCCATTCCCTGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTTGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACCGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGACGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAAGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

97.929

100

0.979
  pilM Vibrio cholerae strain A1552

49.708

100

0.503

Multiple sequence alignment