Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LIS82_RS19190 Genome accession   NZ_CP085712
Coordinates   3677439..3678509 (-) Length   356 a.a.
NCBI ID   WP_252239197.1    Uniprot ID   -
Organism   Cytobacillus solani strain F27     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3672439..3683509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS82_RS19145 (LIS82_19200) - 3672919..3673731 (+) 813 WP_053476991.1 YqhG family protein -
  LIS82_RS19150 (LIS82_19205) - 3673769..3673945 (-) 177 WP_075209182.1 YqzE family protein -
  LIS82_RS19155 (LIS82_19210) - 3674013..3674516 (-) 504 WP_053476992.1 shikimate kinase -
  LIS82_RS19160 (LIS82_19215) comGG 3674531..3674914 (-) 384 WP_053476993.1 competence type IV pilus minor pilin ComGG -
  LIS82_RS19165 (LIS82_19220) comGF 3674901..3675383 (-) 483 WP_252239196.1 competence type IV pilus minor pilin ComGF -
  LIS82_RS19170 (LIS82_19225) - 3675337..3675669 (-) 333 WP_053476995.1 hypothetical protein -
  LIS82_RS19175 (LIS82_19230) comGD 3675653..3676093 (-) 441 WP_053476996.1 competence type IV pilus minor pilin ComGD -
  LIS82_RS19180 (LIS82_19235) comGC 3676086..3676421 (-) 336 WP_053476997.1 competence type IV pilus major pilin ComGC -
  LIS82_RS19185 (LIS82_19240) comGB 3676421..3677452 (-) 1032 WP_053476998.1 competence type IV pilus assembly protein ComGB -
  LIS82_RS19190 (LIS82_19245) comGA 3677439..3678509 (-) 1071 WP_252239197.1 competence type IV pilus ATPase ComGA Machinery gene
  LIS82_RS19195 (LIS82_19250) - 3678811..3679512 (+) 702 WP_053477000.1 helix-turn-helix domain-containing protein -
  LIS82_RS19200 (LIS82_19255) - 3679604..3679846 (+) 243 WP_053477001.1 DUF2626 domain-containing protein -
  LIS82_RS19205 (LIS82_19260) - 3679908..3680969 (-) 1062 WP_252239198.1 SAM-dependent methyltransferase -
  LIS82_RS19210 (LIS82_19265) - 3681478..3682113 (-) 636 WP_053477003.1 MBL fold metallo-hydrolase -
  LIS82_RS19215 (LIS82_19270) - 3682400..3682573 (+) 174 WP_075209188.1 DUF2759 domain-containing protein -
  LIS82_RS19220 (LIS82_19275) - 3682824..3683138 (-) 315 WP_053477004.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40210.66 Da        Isoelectric Point: 9.2439

>NTDB_id=619492 LIS82_RS19190 WP_252239197.1 3677439..3678509(-) (comGA) [Cytobacillus solani strain F27]
MNTIEKLAERIIKDAVRNHASDIHIVPRRSDTLIQLRLANKLIPRLYLPKEECDRLISHFKFTASMDIGEKRRPQSGANS
YQIDGQMIGFRFSTLPSSHNESLVIRILPQQEQIPFYQISLFPSMTRKLLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHMFNRNVITLEDPIEKENDMVLQVQVNEKAGVSYATGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDARGAIYRLSEFGVDAVEIEQTLVAVTAQRLVELSCPFCKGECSPYCYCYGRLKRASIFELLTGRALNASMKVARGEE
AVIKYRNLKDIIKKGIALGFIKETEYERWVLKHDQT

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=619492 LIS82_RS19190 WP_252239197.1 3677439..3678509(-) (comGA) [Cytobacillus solani strain F27]
TTGAATACAATTGAAAAGCTTGCAGAAAGAATTATTAAAGATGCTGTCAGAAATCATGCATCAGATATCCACATTGTTCC
CCGCAGAAGCGATACCCTCATCCAATTACGTCTTGCTAACAAACTAATTCCCAGACTCTATTTGCCGAAAGAAGAATGCG
ACAGACTGATTTCACACTTTAAATTTACGGCTTCAATGGATATCGGTGAAAAGAGAAGACCGCAAAGCGGTGCAAACTCC
TATCAAATTGATGGTCAAATGATCGGCTTTCGCTTTTCAACGCTCCCCTCTAGTCATAATGAAAGCCTAGTTATTCGAAT
CCTCCCTCAGCAAGAACAAATTCCTTTCTACCAAATTTCTTTATTCCCGAGTATGACCCGAAAACTACTTGCATTATTAA
AGCATGCCCACGGATTAATCATATTTACTGGTCCGACTGGATCAGGTAAAACTACCACCCTATACTCGCTTCTCAATGAA
ACCTCACATATGTTCAATCGCAATGTCATCACCCTTGAAGACCCAATTGAAAAAGAAAATGATATGGTGCTCCAGGTTCA
AGTGAATGAAAAAGCTGGTGTGTCCTATGCAACTGGATTAAAAGCCATTCTAAGACATGATCCAGATATCATTATGGTGG
GGGAAATCAGAGATGCAGAAACGGCAAAGATAGCAGTAAGAGCTGCACTAACAGGTCATTTAGTTCTTTCAACGATGCAT
ACGAGGGATGCTAGAGGAGCGATTTACAGGTTGAGTGAATTTGGTGTGGATGCAGTAGAAATAGAACAGACCCTTGTTGC
TGTAACCGCGCAGAGACTTGTGGAATTATCCTGTCCGTTTTGTAAGGGGGAATGTTCCCCATACTGCTATTGCTATGGCA
GATTGAAAAGAGCAAGTATTTTTGAGCTTTTAACTGGGAGGGCATTAAATGCCTCTATGAAAGTGGCGAGAGGTGAAGAG
GCTGTTATTAAATACCGAAACTTAAAGGATATCATTAAAAAGGGAATAGCACTTGGTTTTATTAAAGAAACAGAATATGA
GCGGTGGGTGCTTAAGCATGATCAAACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.84

98.596

0.551
  pilB Glaesserella parasuis strain SC1401

39.773

98.876

0.393
  pilB Haemophilus influenzae 86-028NP

37.069

97.753

0.362