Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HQJ03_RS20440 Genome accession   NZ_CP085510
Coordinates   3999923..4000966 (-) Length   347 a.a.
NCBI ID   WP_173602409.1    Uniprot ID   -
Organism   Bacillus cereus strain HD1.4B     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3994923..4005966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQJ03_RS20395 (HQJ03_020395) - 3995356..3995556 (-) 201 WP_000106083.1 YqzE family protein -
  HQJ03_RS20400 (HQJ03_020400) - 3995595..3996092 (-) 498 WP_173602410.1 shikimate kinase -
  HQJ03_RS20405 (HQJ03_020405) - 3996208..3996858 (-) 651 WP_000183856.1 2OG-Fe(II) oxygenase -
  HQJ03_RS20410 (HQJ03_020410) comGG 3997033..3997404 (-) 372 WP_001231317.1 competence type IV pilus minor pilin ComGG -
  HQJ03_RS20415 (HQJ03_020415) comGF 3997401..3997871 (-) 471 WP_000923101.1 competence type IV pilus minor pilin ComGF -
  HQJ03_RS20420 (HQJ03_020420) - 3997841..3998143 (-) 303 WP_173602424.1 type II secretion system protein -
  HQJ03_RS20425 (HQJ03_020425) comGD 3998136..3998591 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  HQJ03_RS20430 (HQJ03_020430) comGC 3998588..3998887 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  HQJ03_RS20435 (HQJ03_020435) comGB 3998899..3999930 (-) 1032 WP_000475187.1 competence type IV pilus assembly protein ComGB -
  HQJ03_RS20440 (HQJ03_020440) comGA 3999923..4000966 (-) 1044 WP_173602409.1 competence type IV pilus ATPase ComGA Machinery gene
  HQJ03_RS20445 (HQJ03_020445) - 4001172..4001867 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  HQJ03_RS20450 (HQJ03_020450) - 4001993..4002235 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  HQJ03_RS20455 (HQJ03_020455) - 4002343..4003737 (+) 1395 WP_001094345.1 L-cystine transporter -
  HQJ03_RS20460 (HQJ03_020460) - 4003816..4004208 (-) 393 WP_000875394.1 hypothetical protein -
  HQJ03_RS20465 (HQJ03_020465) - 4004296..4004511 (-) 216 WP_001008327.1 DUF3912 family protein -
  HQJ03_RS20470 (HQJ03_020470) - 4004764..4005075 (+) 312 WP_001093239.1 hypothetical protein -
  HQJ03_RS20475 (HQJ03_020475) - 4005110..4005598 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39123.55 Da        Isoelectric Point: 8.7212

>NTDB_id=618734 HQJ03_RS20440 WP_173602409.1 3999923..4000966(-) (comGA) [Bacillus cereus strain HD1.4B]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGVIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=618734 HQJ03_RS20440 WP_173602409.1 3999923..4000966(-) (comGA) [Bacillus cereus strain HD1.4B]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGTAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369