NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	HQN47_RS11750	Genome accession	NZ_CP085504
Coordinates	2428883..2429953 (-)	Length	356 a.a.
NCBI ID	WP_012117985.1	Uniprot ID	A0A411A7V3
Organism	Bacillus velezensis strain BP1.2A
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2423883..2434953

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HQN47_RS11700 (HQN47_011700)	sipW	2423943..2424527 (-)	585	WP_012117977.1	signal peptidase I SipW	-
HQN47_RS11705 (HQN47_011705)	tapA	2424499..2425170 (-)	672	WP_012117978.1	amyloid fiber anchoring/assembly protein TapA	-
HQN47_RS11710 (HQN47_011710)	-	2425429..2425758 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
HQN47_RS11715 (HQN47_011715)	-	2425798..2425977 (-)	180	WP_003153093.1	YqzE family protein	-
HQN47_RS11720 (HQN47_011720)	comGG	2426034..2426411 (-)	378	WP_012117980.1	competence type IV pilus minor pilin ComGG	Machinery gene
HQN47_RS11725 (HQN47_011725)	comGF	2426412..2426912 (-)	501	WP_012117981.1	competence type IV pilus minor pilin ComGF	-
HQN47_RS11730 (HQN47_011730)	comGE	2426821..2427135 (-)	315	WP_012117982.1	competence type IV pilus minor pilin ComGE	-
HQN47_RS11735 (HQN47_011735)	comGD	2427119..2427556 (-)	438	WP_012117983.1	competence type IV pilus minor pilin ComGD	Machinery gene
HQN47_RS11740 (HQN47_011740)	comGC	2427546..2427854 (-)	309	WP_003153087.1	competence type IV pilus major pilin ComGC	Machinery gene
HQN47_RS11745 (HQN47_011745)	comGB	2427859..2428896 (-)	1038	WP_012117984.1	competence type IV pilus assembly protein ComGB	Machinery gene
HQN47_RS11750 (HQN47_011750)	comGA	2428883..2429953 (-)	1071	WP_012117985.1	competence type IV pilus ATPase ComGA	Machinery gene
HQN47_RS11755 (HQN47_011755)	-	2430150..2431100 (-)	951	WP_007408319.1	magnesium transporter CorA family protein	-
HQN47_RS11760 (HQN47_011760)	-	2431246..2432547 (+)	1302	WP_012117986.1	hemolysin family protein	-
HQN47_RS11765 (HQN47_011765)	-	2432585..2433415 (-)	831	WP_012117987.1	STAS domain-containing protein	-
HQN47_RS11775 (HQN47_011775)	-	2433622..2434002 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
HQN47_RS11780 (HQN47_011780)	-	2434239..2434484 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40557.86 Da Isoelectric Point: 9.4784

                                >NTDB_id=618549 HQN47_RS11750 WP_012117985.1 2428883..2429953(-) (comGA) [Bacillus velezensis strain BP1.2A]

MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=618549 HQN47_RS11750 WP_012117985.1 2428883..2429953(-) (comGA) [Bacillus velezensis strain BP1.2A]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTCTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.303	100	0.823
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0618549