Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LIS78_RS16850 Genome accession   NZ_CP085442
Coordinates   3285482..3285904 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain KF18     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3280482..3290904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS78_RS16825 (LIS78_16840) - 3280679..3281500 (+) 822 WP_209150128.1 histidine kinase -
  LIS78_RS16830 (LIS78_16845) - 3281501..3282142 (+) 642 WP_195782618.1 response regulator transcription factor -
  LIS78_RS16835 (LIS78_16850) - 3282318..3283304 (+) 987 WP_252284024.1 glycerophosphodiester phosphodiesterase family protein -
  LIS78_RS16840 (LIS78_16855) - 3283465..3284847 (+) 1383 WP_252284025.1 PepSY domain-containing protein -
  LIS78_RS16845 (LIS78_16860) - 3284877..3285341 (+) 465 WP_252284026.1 FixH family protein -
  LIS78_RS16850 (LIS78_16865) nucA/comI 3285482..3285904 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LIS78_RS16855 (LIS78_16870) - 3286057..3286410 (+) 354 WP_245210603.1 DUF1516 family protein -
  LIS78_RS16860 (LIS78_16875) - 3286449..3287039 (-) 591 WP_195782613.1 hypothetical protein -
  LIS78_RS16865 (LIS78_16880) - 3287197..3287853 (-) 657 WP_252284027.1 TetR/AcrR family transcriptional regulator -
  LIS78_RS16870 (LIS78_16885) - 3287877..3289154 (-) 1278 WP_252284028.1 glycosyltransferase -
  LIS78_RS16875 (LIS78_16890) - 3289319..3289804 (-) 486 WP_209150134.1 hypothetical protein -
  LIS78_RS16880 (LIS78_16895) fsa 3289886..3290554 (-) 669 WP_116076436.1 fructose-6-phosphate aldolase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=618141 LIS78_RS16850 WP_013057949.1 3285482..3285904(+) (nucA/comI) [Priestia megaterium strain KF18]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=618141 LIS78_RS16850 WP_013057949.1 3285482..3285904(+) (nucA/comI) [Priestia megaterium strain KF18]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCAGCTGTTTGTACAATTGACCGAGACGGGGCTGAGGAAAACCGCCGTGAATCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGAGCAGATAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCGGGGTCTTGGATCTCCCATCAAGTAGATGAGTTTCCGGACGGCACGA
AGGTGTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507