Detailed information NTG0617858

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: LIT28_RS05600
• Genome accession: NZ_CP085409
• Coordinates: 1095754..1099479 (+)
• Length: 1241 a.a.
• NCBI ID: WP_242250954.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain KF1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1090754..1104479

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LIT28_RS05585 (LIT28_05585)	-	1090912..1091505 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
LIT28_RS05590 (LIT28_05590)	lepB	1091562..1092125 (+)	564	WP_063224645.1	signal peptidase I	-
LIT28_RS05595 (LIT28_05595)	addB	1092242..1095757 (+)	3516	WP_242250952.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
LIT28_RS05600 (LIT28_05600)	addA	1095754..1099479 (+)	3726	WP_242250954.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
LIT28_RS05605 (LIT28_05605)	-	1099492..1099779 (+)	288	WP_242250956.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
LIT28_RS05610 (LIT28_05610)	gerPF	1099834..1100049 (-)	216	WP_001141570.1	spore germination protein GerPF	-
LIT28_RS05615 (LIT28_05615)	-	1100092..1100478 (-)	387	WP_063224641.1	spore germination protein GerPE	-
LIT28_RS05620 (LIT28_05620)	gerPD	1100494..1100688 (-)	195	WP_001052807.1	spore germination protein GerPD	-
LIT28_RS05625 (LIT28_05625)	gerPC	1100695..1101309 (-)	615	WP_242250958.1	spore germination protein GerPC	-
LIT28_RS05630 (LIT28_05630)	gerPB	1101377..1101583 (-)	207	WP_063224639.1	spore germination protein GerPB	-
LIT28_RS05635 (LIT28_05635)	gerPA	1101598..1101819 (-)	222	WP_001111186.1	spore germination protein GerPA	-
LIT28_RS05640 (LIT28_05640)	-	1101916..1102095 (-)	180	WP_063224638.1	aspartyl-phosphate phosphatase Spo0E family protein	-
LIT28_RS05645 (LIT28_05645)	-	1102342..1103241 (+)	900	WP_063224637.1	fumarylacetoacetate hydrolase family protein	-
LIT28_RS05650 (LIT28_05650)	-	1103275..1103559 (-)	285	WP_063224636.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142722.98 Da
• Isoelectric Point: 4.8054

>NTDB_id=617858 LIT28_RS05600 WP_242250954.1 1095754..1099479(+) (addA) [Bacillus thuringiensis strain KF1]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNAIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQIETAEAHI
RKATELAMLPDGPAPRVETLQTDLALLGTLSSAARESWTSVYEAMQSVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVLDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEVYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHIDLKKPITIEVLQEQIAGMVNKELLTFEQAEEITIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=617858 LIT28_RS05600 WP_242250954.1 1095754..1099479(+) (addA) [Bacillus thuringiensis strain KF1]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGAAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATATTTTAGTAGCAGCAGCAGCCGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAACG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCGGCGCAAGAGATGAAAAATAGAATC
GGAGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCTATTTCGACGATCCATTCATTTTGTTTACAAGTCATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAACGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATGCGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAGGCATACGATGTGGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCGTATTTATTAGAAGATGTGAAATTCCAGATTGAAACGGCGGAAGCGCATATT
CGTAAAGCGACTGAACTTGCAATGCTTCCTGACGGTCCAGCACCTCGCGTTGAAACCCTGCAAACAGACTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCATGGACAAGTGTGTATGAAGCGATGCAGAGTGTATCGTGGCAAACGTTAA
AACGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTGCAAGAAGAGCTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCAGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATTTATCGTTTCCGATTAGCAG
AACCAGGACTATTCTTAGGAAAATATAAACGTTTCACGCAAGAAGGATTGGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGCTAGCAGGCACGAACTTCATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTACCCAGAAGGTGAAGATGTAGCGGCTGAGCTATTATGTA
TTCAGCAAACGGAAGAAGAAGTACTAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAATTAAAACTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTCTTCCGCGTTATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTGCTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCACTTGAAGAAGAGCAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCACTTTCTGATTTG
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGATTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCGAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACAGCGAGAGCTTTAGGTGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTTCCAGTTGTATTTGTAGCTGGACTTGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAGTTCATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTATTATACGTAGCATTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTGAAAGATGCAACTAAGGAAATGGAGAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGATGAAGTTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTAGACGGTAACACGTTACTTGCACCAGAGCCAGTTCAAGAAGAGAAGCAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCACGCTTTATGGAGAAGAAAGGGCTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATATTGATTTGAAGAAGCCGATTACGATTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACTTTTGAACAAGCAGAAGAAATTACGATTGAAAAAGTCATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCCTTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGCGACGAAACGATTCTTGTCCAAGGTGTTATCGACTGTATGATTGAAGAGGAAGATG
GCATCACGTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGTGGATTCGATCAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGCTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTAAAAGAGAAATG
TTTATACTTCTTTGATGGCAATCACGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.488	100	0.537