Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   LIT28_RS05595 Genome accession   NZ_CP085409
Coordinates   1092242..1095757 (+) Length   1171 a.a.
NCBI ID   WP_242250952.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain KF1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1087242..1100757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT28_RS05555 (LIT28_05555) - 1087391..1088182 (-) 792 WP_242250948.1 phosphotransferase -
  LIT28_RS05560 (LIT28_05560) - 1088202..1088696 (-) 495 WP_242250949.1 DUF6584 family protein -
  LIT28_RS05565 (LIT28_05565) cspA 1088981..1089184 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  LIT28_RS05570 (LIT28_05570) - 1089286..1089561 (+) 276 WP_063224649.1 hypothetical protein -
  LIT28_RS05575 (LIT28_05575) - 1089588..1089773 (-) 186 WP_063224648.1 hypothetical protein -
  LIT28_RS05580 (LIT28_05580) - 1090056..1090637 (+) 582 WP_242250950.1 competence protein ComK -
  LIT28_RS05585 (LIT28_05585) - 1090912..1091505 (+) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  LIT28_RS05590 (LIT28_05590) lepB 1091562..1092125 (+) 564 WP_063224645.1 signal peptidase I -
  LIT28_RS05595 (LIT28_05595) addB 1092242..1095757 (+) 3516 WP_242250952.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LIT28_RS05600 (LIT28_05600) addA 1095754..1099479 (+) 3726 WP_242250954.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LIT28_RS05605 (LIT28_05605) - 1099492..1099779 (+) 288 WP_242250956.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  LIT28_RS05610 (LIT28_05610) gerPF 1099834..1100049 (-) 216 WP_001141570.1 spore germination protein GerPF -
  LIT28_RS05615 (LIT28_05615) - 1100092..1100478 (-) 387 WP_063224641.1 spore germination protein GerPE -
  LIT28_RS05620 (LIT28_05620) gerPD 1100494..1100688 (-) 195 WP_001052807.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134207.31 Da        Isoelectric Point: 5.1758

>NTDB_id=617857 LIT28_RS05595 WP_242250952.1 1092242..1095757(+) (addB) [Bacillus thuringiensis strain KF1]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNQAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWISEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDIMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGGTASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=617857 LIT28_RS05595 WP_242250952.1 1092242..1095757(+) (addB) [Bacillus thuringiensis strain KF1]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGAAAACAATACTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGGTTAGCGTGGAAGGTATTGCAAGAAGTTGGT
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAGGCAGCGGAGCAAAACGGTTTTTTTGAACATCTTGGCAGCATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAATTACTATATGATGATTTTGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTCGAAAAGCTTCCGCAGTCTGAATATGTAAATCAGGCTGAAATTTATATAGATGGATTTCATTCGT
TTTCACCGCAAGAGCTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATTACGTTAACGATAGATGAA
AAGACGTTAGCACAGCCAGTAAATGAACTAGATCTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCGCTTATGGAACAGCCACGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAACGCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGTGAAGCGAGTGTAACGATTAGTACAGCAGCTAATTTG
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGTAGACTTGTGGCGGACGAAGATTATCGTTACCGCGATATTGCAGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGACCGATGTCACATCATCCGCTAGTAGAATGCATTCGGTCAGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAGCAATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGATTTCTGAGGATCCGTGGATGTATCGACGCT
ATCGTTCTCTTGACGATACGAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACAGTTATGCAAATGTGTGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGATGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGCGATTTCTTAT
TTGCGACAGATCATGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACATTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAACATTGATCGTTCTAGGTTATCAAATGTGAAAGCAACATTCGTCATTGGGGTAAATGAAGGTG
TCATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTCTTGGTGCGGCAGGAATTGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACCAGAGCAACTGAGAAATTATA
TATTTCCTGTCCGCTCGCAGACGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCGTTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAACGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTCACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTGCTATCAAGTTTATTCTACCGAA
ATCGTGCGCAGAAATTAAGCACAGTTGTAAGTAGAGATTTATACGGAGATACAATTAAGGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCATTAAGAGAGCGTGATATTTTCAAATTAGATGC
GCCGGATATCGGGGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAGAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAACATCTTTCTGCTGTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGCTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATTATTTTCCGTACGTCAATTATTCTGCGTGAACA
TGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCACTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGTCGTATTGACCGTGTTGATAAGGCAGAAGACGAAAACGGG
ACATTCCTACGTATTATTGACTATAAATCAAGTTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCTCTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACAGACGTGGATGAAAAAAGGCGGCACAGCATCACCAGCTGGTG
TATTGTATTTCCACATTCATAACCCAATCGTTGAGGTGAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGGCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCAGATATTATTTCTGCTGGTTTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTCCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTAATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCGGTTTGTCAGTTCGATGAATCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.063

100

0.492