Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   LIT38_RS07030 Genome accession   NZ_CP085394
Coordinates   1337825..1341583 (+) Length   1252 a.a.
NCBI ID   WP_252217440.1    Uniprot ID   -
Organism   Bacillus sp. CMF12     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1332825..1346583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT38_RS07020 (LIT38_07035) - 1333038..1334192 (-) 1155 WP_243508019.1 MFS transporter -
  LIT38_RS07025 (LIT38_07040) addB 1334341..1337847 (+) 3507 WP_252217437.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LIT38_RS07030 (LIT38_07045) addA 1337825..1341583 (+) 3759 WP_252217440.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LIT38_RS07035 (LIT38_07050) - 1341938..1342249 (+) 312 WP_243508022.1 HNH endonuclease -
  LIT38_RS07040 (LIT38_07055) - 1342285..1342503 (-) 219 WP_243508023.1 spore germination protein -
  LIT38_RS07045 (LIT38_07060) - 1342532..1342918 (-) 387 WP_243508024.1 spore germination protein GerPE -
  LIT38_RS07050 (LIT38_07065) - 1342925..1343101 (-) 177 WP_243508025.1 spore gernimation protein GerPD -
  LIT38_RS07055 (LIT38_07070) gerPC 1343098..1343709 (-) 612 WP_243508026.1 spore germination protein GerPC -
  LIT38_RS07060 (LIT38_07075) - 1343740..1343979 (-) 240 WP_243508027.1 spore germination protein GerPB -
  LIT38_RS07065 (LIT38_07080) - 1343993..1344214 (-) 222 WP_009331280.1 spore germination protein -
  LIT38_RS07070 (LIT38_07085) - 1344421..1346013 (+) 1593 WP_252217442.1 long-chain-fatty-acid--CoA ligase -

Sequence

Protein


Download         Length: 1252 a.a.        Molecular weight: 143254.30 Da        Isoelectric Point: 4.9619

>NTDB_id=617500 LIT38_RS07030 WP_252217440.1 1337825..1341583(+) (addA) [Bacillus sp. CMF12]
MKMAIPPKPEDATWTDDQWKAIMAKGQDILVAAAAGSGKTAVLVERIINKIIADEDPINVDELLVVTFTNASAAEMRHRI
GEALEKAINADPKSAHLRKQVSLLNRASISTLHSFCLEMIRKYYYMTDIDPGFRIADETEAQLLRDEVLEELFEEEYGRE
GNEAFFALVDTFTNDRSDTALQDIIRDLYDFARSNPSPDQYLDSIVEMYNVDGVNSLEQLPFVRSLLADIDLQLKGAKQL
LEYGLELTKLPGGPAPRAENFIADLHVVNTLIEAKNDSWLMLYEAMQSWSFGRAKTCKGDDYDEGLVEKAKKLRDQAKKI
ITGLQEELFFRRQESFMRDMAEMKPHIESLVSLVKEFSIRFDQVKTEKGLVDFADLEHYCLEILSSSDEEGRLLPSEAAL
ACKNQFREVLVDEYQDTNMVQEAILQLVTADSEENGNLFMVGDVKQSIYRFRLAEPNLFLGKYTRFGRDGEEAGLRIDLA
RNFRSRKEVLHGTNYIFKQIMGTSVGEIEYDQAAELVKGAPYSESQEHPVEIAIIDLEGNEKNEPEPKSMDEGFDSEDLA
QSQLEARFMASKIKEIIENRKEVYNTKTQSGRPAKYRDIVILLRSMTWAPQIMEEFKQQGIPVYANLSSGYFEATEVAIM
MSLLKVIDNPYQDIPLASVLRSPILGLTEEELAQIRIHNKRGSFYEALTSFCRNDPTLETGELYEKVRPFFDQLKDWRVM
ARQGSLSELIWHLYRETRFFDFVGGMPGGKQRQANLRALYDRARQYEATSFRGLFRFLRFIERMRDRGDDLGAARALGEQ
EDVVRLMTIHSSKGLEFPFVFIAGLARTFNTMDLKKPYMLDKEFGFAAKYVNAEKRISYPSLPQIAFKRKKKMEMLAEEM
RVLYVALTRAKEKLFLVSSVKSADKKINQWLQASEHKEWLLNEYDRASANSYLDWIGPSLVRHKDCEALRGEGPVYPLVP
ADILEHPSCWNITIIKSEEAAVLSEEAKDGETDLLPLVYEGKPVPAESTYKDKVEEQLSWKYSYKQAAQHRSKQSVSEVK
RQREIFSEEDSGTELIRKINKPLLGRPRFMQEKSLSPAERGTAMHAVMQHIDFSRPATVETISSKMAEMVHNELLTEEQH
ASIEPLLIVQFFETELGQRIVQAKSVRREIPFSLSFPAREIYSDWKGGAEPVLIQGIVDCVFEDEQGLVLLDYKTDGITG
RFKGGFAEAKPVLENRYKVQIDMYTRALEQILKREVNERCLFFFDGAHTIKL

Nucleotide


Download         Length: 3759 bp        

>NTDB_id=617500 LIT38_RS07030 WP_252217440.1 1337825..1341583(+) (addA) [Bacillus sp. CMF12]
ATGAAAATGGCCATACCGCCTAAGCCGGAAGATGCAACCTGGACTGATGACCAATGGAAAGCGATTATGGCCAAGGGCCA
GGATATCCTGGTGGCTGCTGCAGCCGGATCAGGGAAAACGGCTGTTTTGGTTGAAAGGATTATCAATAAGATTATCGCTG
ATGAAGATCCTATTAATGTCGATGAACTGCTTGTTGTGACCTTCACAAATGCCTCGGCAGCCGAAATGAGGCACAGAATC
GGCGAGGCTCTTGAAAAAGCGATAAATGCCGATCCGAAGTCAGCTCACCTCAGAAAGCAGGTAAGCTTATTAAACAGGGC
ATCCATCTCGACTTTGCATTCATTTTGCTTAGAGATGATCCGAAAATATTACTACATGACCGATATTGATCCCGGATTTC
GAATCGCTGATGAAACAGAAGCACAGCTCTTAAGGGATGAAGTACTTGAAGAGCTTTTTGAAGAGGAATACGGTAGAGAG
GGAAATGAAGCATTCTTTGCACTTGTTGACACTTTTACAAATGACAGGAGTGACACTGCGCTTCAGGATATAATTAGGGA
CTTATACGATTTTGCCCGCTCCAACCCTTCACCTGATCAATATCTTGATTCAATTGTTGAGATGTATAACGTGGATGGAG
TTAACAGTCTTGAACAGCTTCCATTTGTCCGTTCCCTTTTGGCAGATATAGATCTTCAGCTGAAAGGGGCCAAGCAGCTG
CTGGAATATGGGCTGGAGCTGACCAAGCTTCCAGGAGGACCGGCTCCGAGAGCAGAAAACTTTATCGCAGATCTGCACGT
CGTAAATACTCTAATTGAAGCGAAAAATGATTCTTGGCTGATGCTATATGAAGCGATGCAATCATGGTCTTTTGGCAGAG
CCAAAACGTGTAAGGGTGATGATTATGATGAAGGGCTGGTTGAAAAAGCAAAGAAACTTAGGGACCAGGCCAAGAAAATC
ATAACAGGCCTGCAGGAAGAATTGTTTTTCCGCAGACAGGAAAGCTTCATGCGTGACATGGCGGAAATGAAGCCACATAT
AGAATCGCTTGTTTCGTTAGTTAAAGAATTTTCCATTCGCTTTGACCAGGTTAAAACTGAAAAAGGGCTGGTTGACTTTG
CCGATCTTGAGCATTATTGCCTTGAAATTCTTTCATCATCAGATGAAGAGGGGAGGCTTTTGCCATCAGAAGCAGCTCTT
GCCTGCAAAAATCAGTTCAGAGAAGTTCTTGTTGATGAGTATCAGGATACCAACATGGTTCAGGAAGCTATTTTGCAGCT
GGTAACGGCAGATTCAGAAGAAAACGGGAATCTTTTCATGGTAGGCGATGTAAAGCAGTCGATCTACCGTTTCCGCCTTG
CAGAGCCTAATCTGTTTTTGGGTAAGTACACCCGGTTTGGCCGGGACGGAGAAGAGGCGGGGCTCAGAATTGATTTGGCA
CGCAATTTCAGAAGCCGTAAAGAAGTCCTGCATGGGACCAACTATATTTTTAAGCAAATTATGGGAACATCTGTCGGTGA
AATTGAGTATGATCAGGCAGCTGAGCTTGTTAAAGGAGCTCCTTATTCAGAGAGTCAGGAACACCCTGTAGAAATTGCCA
TTATTGACCTTGAAGGAAATGAAAAAAACGAACCTGAGCCTAAATCAATGGATGAGGGTTTTGACAGCGAAGATTTAGCC
CAGTCACAGCTCGAAGCCAGATTTATGGCGTCGAAAATAAAAGAAATCATTGAGAATCGGAAAGAAGTTTACAACACAAA
GACCCAATCTGGGCGGCCTGCCAAGTATAGGGATATTGTCATCCTGCTACGTTCCATGACATGGGCTCCGCAAATAATGG
AGGAGTTCAAACAGCAGGGAATCCCTGTTTATGCCAATCTCTCCAGCGGCTATTTTGAAGCAACGGAAGTAGCGATTATG
ATGTCACTTCTAAAAGTGATTGATAATCCGTATCAGGATATTCCGCTGGCATCTGTTCTAAGATCTCCTATTTTGGGGCT
GACCGAAGAAGAGCTTGCACAAATCAGGATCCATAATAAAAGAGGATCTTTCTATGAAGCATTGACTTCTTTTTGCCGGA
ATGACCCAACCCTGGAAACAGGAGAATTATATGAAAAAGTGCGGCCATTTTTTGATCAGCTTAAAGATTGGCGTGTGATG
GCGCGCCAGGGTTCTCTTTCCGAGCTGATTTGGCATCTGTATAGAGAAACCAGGTTTTTTGATTTCGTGGGCGGCATGCC
GGGCGGAAAACAGAGGCAGGCAAACCTTCGCGCATTGTACGACAGAGCAAGACAGTATGAAGCAACATCATTCAGAGGAT
TATTCCGCTTCTTAAGGTTTATTGAACGAATGCGGGACAGAGGCGATGATCTTGGCGCTGCCCGTGCTTTGGGTGAGCAG
GAAGATGTTGTCCGCCTTATGACGATTCACAGCAGCAAAGGACTGGAATTTCCTTTCGTTTTCATTGCAGGGCTTGCCCG
CACCTTTAATACGATGGACCTGAAAAAGCCATACATGCTTGATAAGGAATTTGGATTCGCGGCAAAATATGTAAATGCCG
AGAAAAGAATTTCTTATCCCTCTTTGCCGCAGATTGCCTTTAAACGAAAAAAGAAAATGGAAATGCTTGCTGAAGAAATG
CGGGTCCTATATGTTGCTTTAACAAGGGCGAAGGAAAAGCTTTTCCTTGTTTCGTCCGTCAAGAGTGCAGACAAAAAAAT
AAATCAGTGGCTGCAGGCATCTGAGCATAAAGAGTGGCTCCTGAATGAGTATGACAGGGCTTCTGCAAACAGTTACCTGG
ACTGGATTGGCCCATCGCTTGTAAGACATAAAGATTGTGAGGCATTGAGAGGGGAAGGGCCGGTGTATCCTCTCGTTCCG
GCTGACATACTTGAACACCCTTCCTGCTGGAATATAACCATAATCAAAAGCGAAGAAGCTGCTGTTCTTTCAGAAGAAGC
CAAAGATGGCGAAACGGATCTGCTGCCATTGGTTTATGAAGGTAAACCTGTTCCGGCTGAATCGACTTATAAGGATAAAG
TAGAGGAACAGCTTTCCTGGAAATATTCCTATAAGCAAGCAGCACAGCATCGATCTAAACAATCCGTTTCGGAAGTGAAG
AGGCAGCGGGAGATTTTTTCTGAAGAAGACAGCGGTACTGAATTAATCCGTAAAATCAATAAACCCCTGCTAGGAAGGCC
TCGATTTATGCAGGAAAAATCACTTTCTCCAGCTGAACGGGGTACTGCTATGCATGCTGTCATGCAGCATATTGATTTCA
GCAGGCCTGCAACTGTGGAAACGATATCTTCCAAAATGGCAGAGATGGTTCATAACGAATTGCTGACAGAAGAGCAGCAT
GCCAGCATTGAGCCGCTGCTGATTGTACAGTTTTTCGAAACCGAACTGGGACAAAGAATTGTTCAGGCTAAATCGGTAAG
AAGAGAAATCCCATTCAGTTTATCTTTCCCAGCCCGGGAAATCTATTCAGATTGGAAGGGAGGAGCTGAACCAGTTCTCA
TTCAGGGGATTGTGGACTGTGTATTTGAAGATGAGCAGGGCTTGGTGCTTCTGGATTATAAAACGGATGGAATCACCGGC
CGATTTAAGGGAGGCTTTGCTGAAGCCAAACCCGTTCTCGAAAACAGGTACAAAGTTCAAATTGATATGTACACCAGAGC
ACTGGAACAAATATTAAAGAGAGAAGTCAATGAAAGATGCCTATTCTTCTTTGACGGGGCTCATACAATTAAGTTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-466)

UvrD-like helicase C-terminal domain (UvrD_C)

(477-898)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1068-1231)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

54.872

100

0.549