Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   LIT38_RS07025 Genome accession   NZ_CP085394
Coordinates   1334341..1337847 (+) Length   1168 a.a.
NCBI ID   WP_252217437.1    Uniprot ID   -
Organism   Bacillus sp. CMF12     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1329341..1342847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIT38_RS07000 (LIT38_07015) - 1329825..1330151 (-) 327 WP_243508015.1 hypothetical protein -
  LIT38_RS07005 (LIT38_07020) fabG 1330406..1331146 (+) 741 WP_243508016.1 3-oxoacyl-ACP reductase FabG -
  LIT38_RS07010 (LIT38_07025) - 1331202..1332380 (+) 1179 WP_243508017.1 thiolase family protein -
  LIT38_RS07015 (LIT38_07030) - 1332630..1333004 (+) 375 WP_252217435.1 GNAT family protein -
  LIT38_RS07020 (LIT38_07035) - 1333038..1334192 (-) 1155 WP_243508019.1 MFS transporter -
  LIT38_RS07025 (LIT38_07040) addB 1334341..1337847 (+) 3507 WP_252217437.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LIT38_RS07030 (LIT38_07045) addA 1337825..1341583 (+) 3759 WP_252217440.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LIT38_RS07035 (LIT38_07050) - 1341938..1342249 (+) 312 WP_243508022.1 HNH endonuclease -
  LIT38_RS07040 (LIT38_07055) - 1342285..1342503 (-) 219 WP_243508023.1 spore germination protein -

Sequence

Protein


Download         Length: 1168 a.a.        Molecular weight: 134284.52 Da        Isoelectric Point: 6.0518

>NTDB_id=617499 LIT38_RS07025 WP_252217437.1 1334341..1337847(+) (addB) [Bacillus sp. CMF12]
MTVRLLIGRSGSGKTEHCLNEIRDELRLNPDGDPIIYLVPEQMTFLSEYKLITTPGLGGMIRSQVYSFTRLAWRILQETG
GMSRYHLNSVGISMLIRKIIEDKKDELKLFQRAADKNGFIQQIEQIMTEFKRYCVAPDELAERQQQLSGTGKSNKALQDK
LHDLELIYKNFEESLAGKYTDSEDYFRLLAEKASNSEYLKNAEVYIDGFYSFTPQEYSIVEQLMKQCKNVTITLTLDQPF
KNSAPDDLHLFRMPGENYQTIKEMASRNSLDVEEMILTEQRRWSSPSLRHLEDHFDSRPAEALPGEADIHIGQAVNRRAE
IEGVARKINKLVRTDGYRYRDIAVLARNGQDYHDMIETVFHDYDIPFFIDQKRTMLNHPLVELIRSTLEIINGNWRYEPI
FRAVKTELIFPSRLNAGKLREQMDRLENYVLAYGIQGDKWTKKKRWIYRRIRGLEFDAVAQTDAEKKIEDELSDLRKMIT
GPILRLHRRLKKAADGRGLCEALFLYMEEMDVPVKIEKWKTAEEEKGNLVKSREHGQAWNAIMELLDQYVEMLGEEEVTL
KRFASILDAGLESLRFTLVPPAIDQVMAADLERSRLTDIKAAFVIGLNEGVLPAKFSEDGVLADDDREILNTNGMKLAPG
SRTRLLDEEFIAYKAFVTPSEKLFISYPLSNEEGKALMPSPYIKRLGDLFPDCRQHSFMSDPAELPETEQLEYADNLNTA
LSYLTSQLQLKKRSYPIYDLWWDVYNCYLNNEQWRRPALKVLSSLNYENRTLQLGEDISKELYGDHIQASVSRMELFHSC
PFSHFAQHGLRLRERQVYRLEAPDIGDLFHAALKFIAETVMTQKLSWTDMTREQCEILAKEAVEMLAPKLQNEILLSSNR
HHYIKRKLEQIISRASYVISEHAKSSGFSPVGLELGFGPKGELPSLVFPLKNGTKMELVGRIDRVDKAQDTNGTFLRVID
YKSSSKDLNVSEVYYGIALQMLTYLDIIIAHSNALIGTEADPAGVLYFHVHNPIVSTSKMLTLEDIEQELYKKFKMNGLM
LGNENVIRLMDQTLETGDSSIISAGFKKDGTLSKRSKVASKQEFDYLRRYVRKMYANTGNKITEGAVDIAPYKMKDKTPC
TFCAYKSVCQFDESLDTNDYRLFVPKPKEDMIEIIKKEVEGDENGHTA

Nucleotide


Download         Length: 3507 bp        

>NTDB_id=617499 LIT38_RS07025 WP_252217437.1 1334341..1337847(+) (addB) [Bacillus sp. CMF12]
ATGACCGTACGTTTATTGATAGGCCGTTCCGGAAGCGGCAAAACGGAACACTGCCTAAATGAAATCCGCGATGAACTGCG
GCTTAATCCGGATGGAGATCCAATCATTTATTTAGTTCCGGAACAAATGACGTTCCTTTCAGAATATAAATTAATTACCA
CGCCAGGCCTTGGCGGAATGATACGCAGCCAGGTATATTCTTTCACGCGCCTTGCCTGGAGAATTCTTCAGGAAACAGGA
GGCATGAGCAGATACCATCTTAACAGTGTTGGCATCAGCATGCTAATCCGCAAAATTATCGAAGATAAAAAGGATGAACT
GAAGCTTTTCCAGAGGGCTGCAGATAAAAATGGTTTTATTCAGCAAATTGAACAAATAATGACTGAATTCAAACGTTATT
GTGTAGCTCCCGATGAACTGGCTGAAAGGCAGCAGCAGCTCTCAGGAACAGGGAAATCGAATAAGGCACTTCAGGACAAG
CTGCATGATCTTGAATTAATCTATAAAAATTTTGAAGAATCCCTTGCCGGCAAATATACAGATTCAGAGGATTATTTCCG
CCTGCTTGCTGAAAAAGCCTCCAATTCAGAGTATCTAAAAAATGCCGAGGTTTATATAGATGGCTTTTACAGCTTTACAC
CACAGGAATATTCGATTGTTGAACAGCTGATGAAGCAGTGTAAAAACGTCACTATTACACTGACGCTTGACCAGCCGTTT
AAAAATAGTGCTCCTGATGATCTTCATTTATTCAGAATGCCTGGAGAAAACTATCAGACAATCAAAGAAATGGCTTCCCG
CAACAGTCTGGATGTTGAAGAAATGATCCTTACTGAGCAAAGAAGGTGGAGCAGCCCTTCTCTTAGGCATCTTGAGGACC
ATTTTGACAGCAGGCCTGCTGAGGCATTGCCAGGGGAAGCAGACATTCATATCGGACAGGCTGTTAACAGGAGAGCTGAA
ATCGAGGGCGTTGCCAGAAAAATAAATAAGCTTGTTCGTACAGATGGCTATCGCTATCGTGATATTGCAGTACTTGCAAG
AAACGGACAGGATTATCATGACATGATTGAAACAGTGTTCCATGACTATGATATCCCATTTTTTATTGACCAAAAAAGAA
CTATGCTGAATCATCCCCTCGTAGAGTTAATTCGGTCAACTCTTGAAATCATCAATGGGAATTGGCGCTATGAACCGATC
TTCAGGGCAGTAAAAACGGAGCTGATTTTTCCGTCCAGGTTAAACGCCGGCAAGCTTCGTGAGCAAATGGACAGACTTGA
GAACTATGTTCTTGCCTATGGCATTCAAGGAGACAAATGGACGAAGAAGAAGAGATGGATTTACAGGAGAATCAGGGGGC
TGGAGTTTGATGCGGTTGCCCAGACTGATGCCGAGAAGAAAATAGAAGATGAGTTAAGTGATTTGCGCAAAATGATTACA
GGCCCAATATTAAGGCTGCACAGGCGCTTGAAAAAGGCTGCTGATGGCCGCGGACTTTGTGAAGCTTTATTTTTGTACAT
GGAAGAAATGGATGTGCCTGTGAAGATTGAAAAGTGGAAAACAGCTGAAGAGGAAAAAGGGAACCTTGTTAAGTCAAGGG
AACATGGACAAGCATGGAATGCCATTATGGAGCTTCTGGATCAGTATGTAGAAATGCTTGGTGAAGAAGAAGTGACGCTT
AAGCGCTTTGCTTCCATTCTGGATGCCGGTCTGGAGTCGCTGAGATTTACGCTTGTTCCGCCTGCCATCGATCAGGTCAT
GGCGGCCGATTTGGAAAGGTCAAGGCTAACTGATATTAAAGCTGCCTTTGTCATTGGTCTGAATGAGGGAGTACTTCCTG
CCAAATTCTCAGAGGACGGTGTGCTGGCGGATGACGACAGAGAAATTCTGAACACCAACGGAATGAAACTGGCGCCAGGA
AGCCGTACACGACTTCTTGATGAAGAATTTATAGCCTATAAAGCGTTTGTTACTCCATCAGAAAAACTTTTTATCAGTTA
TCCGCTGTCAAATGAAGAAGGTAAGGCTCTTATGCCTTCTCCTTATATTAAGAGATTGGGAGATTTATTCCCTGATTGCA
GGCAGCATTCCTTCATGTCGGATCCGGCAGAGCTGCCGGAAACGGAGCAGCTGGAATATGCGGATAACCTTAATACAGCA
CTTTCGTATTTGACTTCACAGCTTCAGCTGAAAAAACGCAGCTACCCAATCTATGACCTGTGGTGGGATGTTTATAACTG
CTATCTTAATAATGAGCAGTGGAGAAGGCCAGCATTAAAGGTTCTTTCCAGCCTGAATTATGAAAACAGGACACTTCAGC
TTGGCGAAGACATCAGTAAAGAATTGTACGGCGATCATATTCAGGCAAGTGTTTCCAGAATGGAATTATTCCATAGCTGT
CCATTTTCCCATTTTGCACAGCATGGACTAAGGCTGCGGGAAAGACAGGTATACCGCTTGGAGGCACCGGATATCGGGGA
TTTATTCCATGCTGCTTTAAAATTTATCGCAGAAACAGTTATGACTCAGAAGCTTTCATGGACAGATATGACGAGGGAAC
AGTGTGAGATCCTTGCAAAAGAAGCAGTTGAAATGCTTGCTCCTAAACTGCAAAATGAAATTCTTTTAAGCTCGAACAGA
CATCACTATATTAAGAGAAAGCTTGAGCAAATCATCAGCAGGGCCTCCTATGTAATCAGTGAGCATGCCAAATCAAGCGG
ATTTTCGCCAGTGGGGCTTGAGCTGGGATTTGGTCCAAAAGGAGAACTTCCTTCGCTTGTTTTCCCGCTTAAAAACGGGA
CGAAAATGGAACTTGTCGGAAGAATTGACAGGGTGGATAAGGCTCAGGATACAAACGGAACATTTTTACGGGTTATTGAC
TATAAATCCAGTTCCAAAGACTTAAATGTCAGTGAGGTTTATTATGGCATTGCTCTGCAGATGCTTACGTATCTTGATAT
TATCATTGCCCATTCCAATGCCTTAATCGGAACAGAAGCAGATCCAGCTGGAGTACTTTATTTCCATGTCCACAATCCAA
TTGTAAGCACATCTAAGATGCTTACATTGGAAGATATTGAGCAGGAACTTTATAAGAAATTTAAAATGAATGGTCTGATG
CTTGGGAATGAAAATGTTATCAGATTAATGGATCAGACTCTTGAGACAGGAGATTCTTCCATCATCTCTGCAGGATTCAA
GAAGGACGGGACACTCTCGAAGCGATCTAAAGTGGCAAGCAAGCAGGAGTTTGACTATTTGCGCCGTTATGTACGGAAAA
TGTATGCAAATACCGGCAATAAGATTACAGAGGGGGCTGTCGATATTGCCCCGTATAAAATGAAAGACAAAACACCTTGT
ACTTTTTGTGCCTATAAATCTGTATGCCAATTTGACGAATCGCTCGATACAAATGATTACAGATTATTTGTGCCTAAGCC
AAAAGAAGACATGATTGAAATCATCAAGAAGGAGGTAGAGGGCGATGAAAATGGCCATACCGCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(789-1130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

52.222

100

0.523