Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   NUW85_RS03180 Genome accession   NZ_CP102603
Coordinates   742696..745281 (-) Length   861 a.a.
NCBI ID   WP_003154108.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain MBLB0692     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 737696..750281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUW85_RS03165 (NUW85_03165) - 738382..739059 (-) 678 WP_003154103.1 MBL fold metallo-hydrolase -
  NUW85_RS03170 (NUW85_03170) - 740130..740339 (+) 210 WP_003154104.1 hypothetical protein -
  NUW85_RS03175 (NUW85_03175) hexB 740803..742680 (-) 1878 WP_003154106.1 DNA mismatch repair endonuclease MutL Machinery gene
  NUW85_RS03180 (NUW85_03180) hexA 742696..745281 (-) 2586 WP_003154108.1 DNA mismatch repair protein MutS Machinery gene
  NUW85_RS03185 (NUW85_03185) cotE 745400..745945 (-) 546 WP_003154111.1 outer spore coat protein CotE -
  NUW85_RS03190 (NUW85_03190) ymcA 746198..746629 (-) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  NUW85_RS03195 (NUW85_03195) miaB 746632..748161 (-) 1530 WP_003154116.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  NUW85_RS03200 (NUW85_03200) - 748304..749482 (-) 1179 WP_088037143.1 glycine C-acetyltransferase -

Sequence

Protein


Download         Length: 861 a.a.        Molecular weight: 97700.96 Da        Isoelectric Point: 5.3594

>NTDB_id=617381 NUW85_RS03180 WP_003154108.1 742696..745281(-) (hexA) [Bacillus amyloliquefaciens strain MBLB0692]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRNGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEEALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQTGDKPELPAAPEKK
PAVKEEPAQLSFFADGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide


Download         Length: 2586 bp        

>NTDB_id=617381 NUW85_RS03180 WP_003154108.1 742696..745281(-) (hexA) [Bacillus amyloliquefaciens strain MBLB0692]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCGCAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAGCAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACCCCGGGAACCGTGATGGATGGAAAAGGCATCCATGAGTCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTTC
GTAACGGATACGGTCTCGCTTTATCAGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAACGAACATACAGCTGCTCAGCTGAA
AGAACGGTGCGGCGCAACGATTTCTATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCATCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCAATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGCGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCAGGGTCGCATTCGGAAACGT
CAACGCAAGAGATTTAATCCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATTAAAGAGCTTGTAGGATCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGAAGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAACTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAGGACGGGCGGTATGAGCGGAAG
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGTCTGCGTGAAAAGGTCAAACAATTTATTCCGCGTCTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGATGCGTTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCTTGATGGGCGATTCGCGGGAAATGCTCCTGATTACAGGTCCGAATATGTCAGGTAAAAGCACGTACATGAGAC
AGATCGCACTTCTATCGATCATGGCCCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAATATGTTCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGTGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCGGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGCACCTGAGAAAAAG
CCCGCCGTCAAAGAAGAACCGGCACAGCTGTCCTTTTTTGCTGACGGTGAAAAAGAACAGAAGGCACCGGCGGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATCAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

MutS domain I (MutS_I)

(5-116)

MutS domain II (MutS_II)

(125-239)

MutS domain V (MutS_V)

(598-785)

MutS family domain IV (MutS_IV)

(415-506)

MutS domain III (MutS_III)

(258-547)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

48.191

99.535

0.48
  mutS Pseudomonas stutzeri strain ATCC 17587

38.549

100

0.395