Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LIS77_RS00590 Genome accession   NZ_CP085390
Coordinates   115378..116757 (+) Length   459 a.a.
NCBI ID   WP_035332338.1    Uniprot ID   W7L2B7
Organism   Cytobacillus firmus strain CK19     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 110378..121757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIS77_RS00570 (LIS77_00570) - 110494..110955 (+) 462 WP_009336509.1 CtsR family transcriptional regulator -
  LIS77_RS00575 (LIS77_00575) - 110979..111527 (+) 549 WP_048011542.1 UvrB/UvrC motif-containing protein -
  LIS77_RS00580 (LIS77_00580) - 111529..112608 (+) 1080 WP_222501354.1 protein arginine kinase -
  LIS77_RS00585 (LIS77_00585) clpC 112613..115063 (+) 2451 WP_048011540.1 ATP-dependent protease ATP-binding subunit ClpC -
  LIS77_RS00590 (LIS77_00590) radA 115378..116757 (+) 1380 WP_035332338.1 DNA repair protein RadA Machinery gene
  LIS77_RS00595 (LIS77_00595) disA 116760..117833 (+) 1074 WP_048011539.1 DNA integrity scanning diadenylate cyclase DisA -
  LIS77_RS00600 (LIS77_00600) - 118052..119140 (+) 1089 WP_048011538.1 PIN/TRAM domain-containing protein -
  LIS77_RS00605 (LIS77_00605) ispD 119226..119912 (+) 687 WP_197249128.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LIS77_RS00610 (LIS77_00610) ispF 119927..120412 (+) 486 WP_048011536.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49533.99 Da        Isoelectric Point: 7.4342

>NTDB_id=617382 LIS77_RS00590 WP_035332338.1 115378..116757(+) (radA) [Cytobacillus firmus strain CK19]
MAKRKTKFMCQECGYESAKWMGKCPGCGQWNTMVEEVEKPASTRRGAFAHSQGTAIAAKATPITSIETVSEPRIHTDLKE
LNRVLGGGIVRGSLVLIGGDPGIGKSTLLLQVSSQLANRKHSVLYISGEESMRQTKLRADRLGVSSDSLLVYSETSLEEI
SRTIETVNPDFVIIDSIQTIFHPEVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKELGLEEVANPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDKPTKATDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPANNLGGWSAPGGIELIGVSSVGEALKAALGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=617382 LIS77_RS00590 WP_035332338.1 115378..116757(+) (radA) [Cytobacillus firmus strain CK19]
ATGGCTAAAAGAAAAACGAAATTCATGTGCCAGGAATGCGGCTATGAATCAGCGAAATGGATGGGAAAATGTCCGGGGTG
CGGACAATGGAATACGATGGTGGAAGAGGTAGAGAAGCCAGCTTCAACGAGAAGAGGCGCTTTTGCACATTCACAGGGAA
CGGCCATAGCGGCAAAAGCTACGCCGATAACATCCATTGAAACAGTCAGCGAACCCCGCATACATACCGATTTAAAAGAA
TTAAACCGTGTCCTCGGGGGCGGGATTGTAAGAGGTTCCCTTGTTTTGATCGGTGGAGATCCGGGCATCGGGAAATCAAC
GCTTCTTCTTCAAGTGTCCTCACAGCTTGCGAACAGGAAGCATTCAGTCCTCTATATATCAGGGGAAGAATCCATGCGGC
AGACGAAGCTGCGGGCTGACCGTTTGGGTGTTTCTTCAGACAGTCTGCTCGTTTATTCGGAAACCAGCCTTGAAGAAATC
AGCAGAACGATTGAAACAGTCAACCCTGACTTTGTCATTATCGACTCCATACAGACGATTTTTCATCCGGAAGTGACATC
GGCGCCGGGGAGCGTTTCCCAGGTTCGGGAATGTACCGCTGAACTGATGCGCATTGGCAAAACGAAGGGAATTGCCATTT
TTATCGTAGGGCATGTTACAAAGGAGGGCTCCATTGCGGGTCCAAGACTTCTGGAGCACATGGTGGATACGGTTTTATAC
TTTGAAGGTGAACGCCATCATACATACCGGATTTTACGAGCTGTAAAAAACAGATTCGGGTCAACAAACGAGATGGGCAT
TTTTGAGATGAAGGAACTTGGGCTTGAAGAAGTAGCCAATCCATCTGAAATTTTCCTCGAAGAGCGGTCACAAGGGGCTG
CCGGTTCAACAGTTGTTGCTTCCATGGAGGGGACGAGGCCTGTTCTTGTTGAAATTCAGGCGCTTATTTCCCCGACAAGC
TTTGGAAATCCAAGACGAATGGCAACCGGGATCGATCATAACCGGGTCCCGCTCCTGATGGCCGTTTTGGAAAAACGGGT
CGGAATGCTCCTGCAAAATCAGGATGCCTACTTAAAAGTCGCTGGCGGAGTAAAGCTTGATGAACCGGCAATTGATTTGG
CCATAGCGGTCAGCATTGCGTCGAGCTTCCGGGATAAGCCGACAAAAGCAACAGACTGCATTATTGGAGAAGTAGGACTC
ACAGGCGAGGTCAGGAGAGTCTCTAGGATCGAACAAAGAGTACAGGAGGCGGCCAAGCTGGGCTTTGAACGGGTCATCCT
GCCGGCGAACAATCTTGGAGGCTGGAGCGCGCCGGGAGGCATCGAACTGATCGGAGTTAGCTCTGTTGGTGAAGCGCTAA
AGGCTGCGTTAGGGGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(66-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W7L2B7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.609

100

0.778
  radA Streptococcus pneumoniae Rx1

61.488

99.564

0.612
  radA Streptococcus pneumoniae D39

61.488

99.564

0.612
  radA Streptococcus pneumoniae R6

61.488

99.564

0.612
  radA Streptococcus pneumoniae TIGR4

61.488

99.564

0.612
  radA Streptococcus mitis NCTC 12261

61.488

99.564

0.612
  radA Streptococcus mitis SK321

61.488

99.564

0.612