Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LI911_RS02355 Genome accession   NZ_CP085318
Coordinates   437841..438338 (+) Length   165 a.a.
NCBI ID   WP_323147757.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CKFA-361     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432841..443338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LI911_RS02315 - 432885..433871 (+) 987 WP_000161314.1 ROK family glucokinase -
  LI911_RS02320 - 433871..434200 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  LI911_RS02325 - 434197..434820 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  LI911_RS02330 comGA 434872..435846 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  LI911_RS02335 comGB 435818..436888 (+) 1071 WP_086353418.1 competence type IV pilus assembly protein ComGB Machinery gene
  LI911_RS02340 comGC 436902..437213 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  LI911_RS02345 comGD 437191..437637 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  LI911_RS02350 comGE 437624..437923 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  LI911_RS02355 comGF 437841..438338 (+) 498 WP_323147757.1 competence type IV pilus minor pilin ComGF Machinery gene
  LI911_RS02360 - 438435..438581 (+) 147 WP_001789879.1 hypothetical protein -
  LI911_RS02365 - 438571..439095 (+) 525 WP_001015119.1 shikimate kinase -
  LI911_RS02370 gcvT 439254..440345 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  LI911_RS02375 gcvPA 440365..441711 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  LI911_RS02380 gcvPB 441704..443176 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19177.88 Da        Isoelectric Point: 10.0974

>NTDB_id=617147 LI911_RS02355 WP_323147757.1 437841..438338(+) (comGF) [Staphylococcus aureus strain CKFA-361]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTNVDFEFFSRDI
LEDFKGVDRNDIEIRQQHIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=617147 LI911_RS02355 WP_323147757.1 437841..438338(+) (comGF) [Staphylococcus aureus strain CKFA-361]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAAATGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCATATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(70-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

96.97

100

0.97
  comGF Staphylococcus aureus MW2

96.97

100

0.97