Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   NRF20_RS25400 Genome accession   NZ_CP102361
Coordinates   5548818..5550230 (-) Length   470 a.a.
NCBI ID   WP_398628058.1    Uniprot ID   -
Organism   Streptomyces sp. R-74717     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5543818..5555230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRF20_RS25375 (NRF20_25335) - 5543983..5544633 (-) 651 WP_266360524.1 SigE family RNA polymerase sigma factor -
  NRF20_RS25380 (NRF20_25340) - 5544809..5545711 (-) 903 WP_398628061.1 A/G-specific adenine glycosylase -
  NRF20_RS25385 (NRF20_25345) - 5545856..5546500 (-) 645 WP_398628060.1 phosphatase PAP2 family protein -
  NRF20_RS25390 (NRF20_25350) - 5546674..5547504 (+) 831 WP_398628059.1 hypothetical protein -
  NRF20_RS25395 (NRF20_25355) disA 5547613..5548737 (-) 1125 WP_114245192.1 DNA integrity scanning diadenylate cyclase DisA Machinery gene
  NRF20_RS25400 (NRF20_25360) radA/sms 5548818..5550230 (-) 1413 WP_398628058.1 DNA repair protein RadA Machinery gene
  NRF20_RS25405 (NRF20_25365) - 5550471..5552360 (+) 1890 WP_413753315.1 BACON domain-containing protein -
  NRF20_RS25410 (NRF20_25370) - 5552466..5553317 (-) 852 WP_398628056.1 hypothetical protein -
  NRF20_RS25415 (NRF20_25375) - 5553358..5554310 (+) 953 Protein_5010 Ppx/GppA family phosphatase -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 49476.52 Da        Isoelectric Point: 8.2515

>NTDB_id=616147 NRF20_RS25400 WP_398628058.1 5548818..5550230(-) (radA/sms) [Streptomyces sp. R-74717]
MAARTKSAKDRPSYRCTECGWTTAKWLGRCPECQAWGTVEEFGGAPAVRTTAAGRVSTAALPIGQVDSRQATARSTGVGE
LDRVLGGGLVPGAVVLLAGEPGVGKSTLLLDVAAKAANDDHRTLYVTAEESASQVRLRADRIRAINDHLYLAAETDLSAV
LGHLDAVKPSLLILDSVQTVASPEIDGAPGGMAQVREVAGALIRASKERGMSTLLVGHVTKDGAIAGPRLLEHLVDVVLS
FEGDRHARLRLVRGVKNRYGATDEVGCFELHDEGITGLADPSGLFLTRRDEPVPGTCLTVTLEGKRPLVAEVQALTVDSQ
IPSPRRTTSGLETSRVSMMLAVLEQRGRISSLGKRDIYSATVGGVKLSEPAADLAVALALASAASDTPLPKNLVAIGEVG
LAGEVRRVTGVQRRLAEAYRLGFTHALVPTDPGKVPAGMKVTEVANMGDALSVLPRRSRAQAPQEENARR

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=616147 NRF20_RS25400 WP_398628058.1 5548818..5550230(-) (radA/sms) [Streptomyces sp. R-74717]
ATGGCTGCCCGTACGAAATCCGCGAAGGACCGGCCGTCCTACCGCTGCACCGAATGCGGCTGGACGACCGCCAAGTGGCT
CGGCCGCTGCCCCGAATGCCAGGCATGGGGGACGGTCGAGGAGTTCGGCGGCGCCCCCGCCGTGCGGACCACCGCGGCCG
GCCGGGTCAGCACCGCCGCGCTCCCCATCGGCCAGGTCGACAGCCGACAGGCCACCGCCCGGTCGACCGGCGTCGGCGAG
CTGGACCGGGTGCTCGGCGGCGGTCTCGTGCCGGGTGCCGTCGTGCTGCTCGCGGGCGAGCCGGGCGTCGGGAAGTCGAC
GCTGCTGCTGGACGTGGCCGCGAAGGCGGCGAACGACGACCACCGCACGCTCTATGTCACCGCCGAGGAGTCCGCGAGCC
AGGTCCGCCTGCGCGCCGACCGGATCCGGGCGATCAACGACCATCTGTATCTCGCCGCGGAGACCGACCTGTCCGCGGTC
CTCGGCCATCTGGACGCCGTCAAGCCGTCGCTTCTCATCCTCGACTCCGTGCAGACGGTCGCCTCGCCCGAGATCGACGG
GGCGCCCGGCGGCATGGCACAGGTCCGGGAGGTCGCCGGTGCCCTCATCCGGGCCTCCAAGGAACGCGGGATGTCCACGC
TGCTGGTCGGCCATGTCACCAAGGACGGGGCGATCGCCGGGCCGCGGCTGCTGGAGCATCTCGTCGATGTGGTGCTGTCC
TTCGAGGGCGACCGCCATGCCCGCCTGCGGCTCGTACGGGGCGTCAAGAACAGGTACGGCGCGACCGACGAGGTCGGCTG
CTTCGAGCTGCACGACGAGGGCATCACCGGCCTCGCCGACCCGTCCGGGCTGTTCCTCACCCGCCGCGACGAACCCGTGC
CCGGCACCTGCCTGACCGTCACGCTGGAGGGCAAGCGGCCGCTCGTCGCCGAGGTGCAGGCGCTGACGGTCGATTCCCAG
ATCCCTTCGCCCCGGCGCACCACCTCGGGTCTGGAGACGTCCCGGGTGTCGATGATGCTGGCCGTTCTGGAGCAGCGCGG
CCGGATCAGCTCGCTCGGCAAGCGGGACATCTACAGCGCGACGGTCGGCGGTGTGAAGCTCTCCGAGCCCGCCGCGGACC
TCGCGGTCGCGCTGGCGCTGGCCAGTGCCGCGAGTGACACACCACTCCCCAAGAACCTGGTGGCGATCGGCGAAGTAGGC
CTCGCGGGGGAGGTCAGGAGGGTTACGGGAGTCCAGCGCAGACTGGCCGAGGCGTACCGTCTGGGCTTCACGCACGCGCT
GGTTCCGACCGACCCGGGGAAGGTCCCGGCCGGGATGAAGGTCACAGAAGTCGCCAACATGGGTGATGCACTGAGCGTGC
TCCCGCGCCGGTCTCGCGCACAGGCCCCACAGGAGGAGAACGCACGCCGGTAG

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(85-223)

Subunit ChlI of Mg-chelatase (ChlI)

(370-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(14-41)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

43.584

96.17

0.419
  radA Streptococcus pneumoniae Rx1

44.056

91.277

0.402
  radA Streptococcus pneumoniae D39

44.056

91.277

0.402
  radA Streptococcus pneumoniae R6

44.056

91.277

0.402
  radA Streptococcus pneumoniae TIGR4

44.056

91.277

0.402
  radA Streptococcus mitis SK321

44.056

91.277

0.402
  radA Streptococcus mitis NCTC 12261

44.056

91.277

0.402