Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   EKM74_RS01340 Genome accession   NZ_AP019306
Coordinates   264086..264772 (-) Length   228 a.a.
NCBI ID   WP_001802022.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM9463     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259086..269772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKM74_RS01320 - 259995..260534 (-) 540 Protein_256 ComEC/Rec2 family competence protein -
  EKM74_RS01325 - 260633..261805 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  EKM74_RS01330 - 261858..263528 (-) 1671 Protein_258 ComEC/Rec2 family competence protein -
  EKM74_RS01335 - 263533..263994 (-) 462 WP_000439693.1 ComE operon protein 2 -
  EKM74_RS01340 comEA 264086..264772 (-) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  EKM74_RS01345 - 264812..265126 (-) 315 Protein_261 class I SAM-dependent methyltransferase -
  EKM74_RS01350 - 265211..266170 (+) 960 WP_002303350.1 IS30 family transposase -
  EKM74_RS01355 - 266178..266594 (-) 417 Protein_263 class I SAM-dependent DNA methyltransferase -
  EKM74_RS01360 rsfS 266597..266950 (-) 354 WP_001088020.1 ribosome silencing factor -
  EKM74_RS01365 yqeK 266951..267535 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EKM74_RS01370 nadD 267525..268094 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  EKM74_RS01375 yhbY 268097..268387 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  EKM74_RS01380 aroE 268391..269197 (-) 807 WP_000666757.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25900.65 Da        Isoelectric Point: 9.6751

>NTDB_id=61594 EKM74_RS01340 WP_001802022.1 264086..264772(-) (comEA) [Staphylococcus aureus strain TUM9463]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=61594 EKM74_RS01340 WP_001802022.1 264086..264772(-) (comEA) [Staphylococcus aureus strain TUM9463]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

100

100

1
  comEA Staphylococcus aureus MW2

97.368

100

0.974

Multiple sequence alignment