Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LIN97_RS00510 Genome accession   NZ_CP085095
Coordinates   99278..99730 (+) Length   150 a.a.
NCBI ID   WP_005586498.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 5R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 94278..104730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN97_RS00495 pnp 95235..97364 (-) 2130 WP_233119032.1 polyribonucleotide nucleotidyltransferase -
  LIN97_RS00500 nanQ 97658..98128 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  LIN97_RS00505 ampD 98602..99156 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LIN97_RS00510 pilA 99278..99730 (+) 453 WP_005586498.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LIN97_RS00515 pilB 99757..101166 (+) 1410 WP_005567970.1 GspE/PulE family protein Machinery gene
  LIN97_RS00520 pilC 101159..102382 (+) 1224 WP_005567968.1 type II secretion system F family protein Machinery gene
  LIN97_RS00525 - 102379..103068 (+) 690 WP_005547547.1 A24 family peptidase -
  LIN97_RS00530 coaE 103118..103741 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  LIN97_RS00535 yacG 103731..103943 (+) 213 WP_005567966.1 DNA gyrase inhibitor YacG -
  LIN97_RS00540 - 103943..104215 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15547.96 Da        Isoelectric Point: 9.1384

>NTDB_id=615493 LIN97_RS00510 WP_005586498.1 99278..99730(+) (pilA) [Aggregatibacter actinomycetemcomitans strain 5R]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGVITVTGKGSLDKISYTLTASGTAASGVSWNAACGNNADIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=615493 LIN97_RS00510 WP_005586498.1 99278..99730(+) (pilA) [Aggregatibacter actinomycetemcomitans strain 5R]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATCGC
TATTGTTGCTATTCTCGCCACCGTAGCCGTACCATCTTATCAAAATTACACTAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCCCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AACGGCATTCAAGCAGATGTTGCAGATGCTTCGAAACAAAAATATGTGAAATCCACTGCGGTCAAAGGCGGCGTCATCAC
CGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACGGCTGCCAGCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGACATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.319

92

0.573
  pilA Haemophilus influenzae Rd KW20

59.028

96

0.567
  pilA Glaesserella parasuis strain SC1401

53.846

95.333

0.513