Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   LIN99_RS00310 Genome accession   NZ_CP085093
Coordinates   57772..58323 (+) Length   183 a.a.
NCBI ID   WP_005547469.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 23S     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 52772..63323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIN99_RS00295 - 53722..55086 (+) 1365 WP_005547462.1 L-serine ammonia-lyase -
  LIN99_RS00300 ansB 55267..56316 (-) 1050 WP_005568022.1 L-asparaginase 2 -
  LIN99_RS00305 - 56590..57750 (+) 1161 WP_249850156.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  LIN99_RS00310 pilF/pilF2 57772..58323 (+) 552 WP_005547469.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LIN99_RS00315 - 58467..59522 (+) 1056 WP_005568019.1 RodZ family helix-turn-helix domain-containing protein -
  LIN99_RS00320 ispG 59534..60637 (+) 1104 WP_005542474.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LIN99_RS00325 hisS 60659..61936 (+) 1278 WP_005568017.1 histidine--tRNA ligase -
  LIN99_RS00330 - 61947..62561 (+) 615 WP_005547476.1 YfgM family protein -
  LIN99_RS00335 - 62614..63066 (+) 453 WP_005568016.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20667.46 Da        Isoelectric Point: 8.8869

>NTDB_id=615440 LIN99_RS00310 WP_005547469.1 57772..58323(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain 23S]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKAFSYAPDYYLVSAAFAYF
YQQQGDIEQARTAYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPKRAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=615440 LIN99_RS00310 WP_005547469.1 57772..58323(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain 23S]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGAAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.576

90.164

0.519
  pilF Glaesserella parasuis strain SC1401

47.059

92.896

0.437