Detailed information NTG0614803

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: LG951_RS05470
• Genome accession: NZ_CP085037
• Coordinates: 1100779..1104477 (+)
• Length: 1232 a.a.
• NCBI ID: WP_226804981.1
• Uniprot ID: -
• Organism: Bacillus pumilus strain BIM B-171
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1095779..1109477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG951_RS05455 (LG951_05455)	-	1096073..1096483 (+)	411	WP_003211332.1	DoxX family protein	-
LG951_RS05460 (LG951_05460)	-	1096680..1097126 (-)	447	WP_226804977.1	ferritin-like domain-containing protein	-
LG951_RS05465 (LG951_05465)	addB	1097292..1100801 (+)	3510	WP_226804979.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
LG951_RS05470 (LG951_05470)	addA	1100779..1104477 (+)	3699	WP_226804981.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
LG951_RS05475 (LG951_05475)	sbcD	1104504..1105679 (+)	1176	WP_226804983.1	exonuclease subunit SbcD	-
LG951_RS05480 (LG951_05480)	-	1105676..1109071 (+)	3396	WP_226804985.1	AAA family ATPase	-
LG951_RS05485 (LG951_05485)	-	1109091..1109390 (+)	300	WP_226804987.1	HNH endonuclease	-

Sequence

Protein

• Length: 1232 a.a.
• Molecular weight: 142161.14 Da
• Isoelectric Point: 5.1214

>NTDB_id=614803 LG951_RS05470 WP_226804981.1 1100779..1104477(+) (addA) [Bacillus pumilus strain BIM B-171]
MQISKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEHPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEELPFYPYIKEDLSLVLRSCQELLERA
LSLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNVNFKRLKTCKGDEYDPALLEKSTDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAEQDAEGHLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHSAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFQPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESKDTTTEMLLVHLDQHEAETGEEREELETVQFEARIIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENELALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFQLLRKWRDFSINHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEEWLLPDFERYQSKTYLDFIGPALIRHQAMSSVLEETGDIVLSHPSTFTISFTQASDL
LKEDMSLEKKEQDEIVQALIDGLPVEGYGDADEKVAERLSWKYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIGHLLESLQQRDLLTDEQIQSINQEGIASFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDTEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTVDAAEPILKKRYETQ
IALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3699 bp

>NTDB_id=614803 LG951_RS05470 WP_226804981.1 1100779..1104477(+) (addA) [Bacillus pumilus strain BIM B-171]
ATGCAAATATCAAAACCGAATAACAGTACGTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCTT
GGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGCCTGATTCGAAAAATGACCCGGCCTGAACATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCTTCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAAGAATTAGCCAAAAATCCTGGGTCTCTTCATATGAGAAGGCAGCTGTCCCTCATGAATCGAGCCAATATTTC
AACCTTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAAATTGATCTTGATCCAGGCTTTCGTTTGGCTG
ACCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGACGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCATCA
TTCTTTGAATTGGTTGATCGTTATACATCTGACCGTCACGACTTAGATTTGCAATGGCTCGTGAAGAGAATTTATGAGTT
TTCGAGATCTCATCCTTCTCCGGAGCAATGGATGCGGGCATTTCTTTCACTTTATGATGTAGATGCTCAAACAAAAGTAG
AAGAATTACCGTTTTATCCATATATCAAAGAAGATCTTTCTCTCGTTCTCCGGAGCTGCCAGGAGCTGCTTGAACGAGCT
CTTTCGCTATCAAAAGAGCCGGGCGGCCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCCGTCATCAAGATGATTTTGAGAAGCTTTATGAGCTTTTGCCGAACGTCAATTTTAAAAGGCTCAAAACGTGCAAAG
GGGACGAATATGACCCTGCTCTCTTAGAAAAATCCACAGATGCACGTAATCAAGCAAAAAAACAATTAGAAAAACTAAAA
GATGAATACTTCATGCGCAGTCCTGCCCAACATTTAAAAAGCTTAGCTGAAATGAAGCCAGTTGTGGAGACGCTTGTAGA
GCTTGTGATCCAATTTGGTGAGCGTTTCGAAAGAGCGAAGCAGGAAAAGTCAATTGTCGACTTTTCGGATTTAGAGCACT
ATTGCTTACGCATTTTAGCGGAGCAGGATGCAGAAGGACATTTGATCGAAACAGAAGCTGCCAAATACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTATCTAAGGGGAA
ACATTCTGCAGAGGGCAATCTGTTTATGGTTGGTGATGTAAAGCAGTCAATTTATCGTTTTAGGCTGGCAGAGCCTATGC
TCTTTTTAAACAAATATAAACACTTTCAACCAGACGGCAAAGAAACAGGGAAGAGAATCGATTTAAATAAAAACTTCCGT
AGCCGTTCAGATGTTTTAGATAGTACGAACTTTTTGTTCAAGCAGCTGATGGGGGAAACAGTGGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCAAAGATACAACGACAGAAATGCTTCTTGTCCACTTAG
ATCAGCATGAAGCAGAAACCGGCGAAGAGCGAGAAGAGCTCGAGACCGTCCAATTTGAAGCGAGAATCATTGCTAAAAAA
ATAAAAGAATTAGTGGAGCAGCCATTTCAAGTGTACGATGCAAAGCAGCAAATGACCCGTAATTTGCAGTATCGTGACAT
TGTCATTTTGCTTCGGTCTATGCCTTGGGCTCCACAAATGATGGAGGAATTAAAGAAGCAGGGAATCCCTGTATATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTCATTCTTTCATTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCTGCTGTTTTAAGATCACCTATTGTTCATTTAGACGAAAATGAATTGGCTCTGATTCGAACGAGTGA
TAAAAAAGGCACGTATTATGATGCTGTAAAGGCATTCATGAGTGTGACGCATTCTGATCACCCTACATGCAAAAAACTAG
AGCGATTTTTCCAATTACTGCGCAAGTGGCGTGATTTTTCTATCAATCATTCGGTGGCAGAATTAATTTGGGAAGTCTAC
CGTGATACGCAGTATCTTGATTATGTTGGCGGAATGCCTGGAGGTAAGCAGCGACAGGCAAATCTTCGTGCCCTTTATGA
CCGGGCTAAGCAATACGAAAAAGCTGCCTTTAGAGGGCTATTCCGATTCCTTCGTTTTATTGAAAGAATGCAGGAGCGTG
GGGATGATCTTGGTGCAGCAAAAACGTTCAGTGAAACAGAGGACGTTGTGCGTATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGGCTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCTTATTTACTTGA
TAAAGAACTAGGCTTTGGAAGCAAGTACATCCATCCTGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTGTTATCTGAAGAATTAAGGGTCCTATATGTAGCGCTCACAAGGGCTAAAGAAAAATTA
TTTCTAGTCGGCTCTGTCAAAAATCAGGTGAAAGCATTAAGCAAATGGCAAAATGCCGCAACCGGAGAAGAGTGGCTGCT
CCCTGATTTTGAACGGTATCAATCGAAAACATACTTGGATTTCATTGGCCCTGCCCTTATCCGTCATCAAGCGATGTCAT
CTGTTTTAGAAGAAACAGGAGATATTGTTCTTTCGCATCCGTCTACATTTACTATCTCATTTACGCAAGCTTCTGATCTT
TTAAAGGAAGATATGTCACTTGAAAAAAAGGAGCAAGACGAGATTGTACAAGCATTGATCGATGGTCTACCTGTTGAAGG
ATATGGTGATGCTGATGAGAAGGTGGCAGAGAGACTCTCATGGAAATATCCGTATCTAGCGGCCTCGCAAGTCGGCACAA
AGCAGTCTGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTATTCGAAAAGCTCGT
GCAACATTGTATGACCGGCCAGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGCACCGCAATGCATACAGT
GATGCAGCATATCCCGCTCCCTTCTGAGGAGCCCTATGATGAATCACGTATAGGACACCTGCTCGAATCCTTACAGCAGC
GAGACTTGCTAACAGATGAGCAGATCCAATCAATTAATCAAGAAGGAATTGCTTCTTTCTTCTCTACTTCAATTGGTCAA
AAACTGCTAAAAGCGGACTGGGTAAAACGAGAGGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGTCATAT
TGATACTGAGGGAGAGCCTGTTCTGATTCAAGGGATGATTGACTGTTTATTTGAAACAGATGGAAAGCTATATTTACTAG
ATTATAAAACTGATAGAGTACAAGGCAGATATACAGTTGACGCTGCTGAACCGATCTTGAAAAAGCGTTATGAAACCCAA
ATAGCCTTATATGCAAAAGCTGTAGAGCGTTTGACAAACAGAACATTAGAGGAGAAAATTCTCTATTTCTTCGATGGAAA
TTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1224)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-463)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.289	100	0.666