Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   LG951_RS05465 Genome accession   NZ_CP085037
Coordinates   1097292..1100801 (+) Length   1169 a.a.
NCBI ID   WP_226804979.1    Uniprot ID   -
Organism   Bacillus pumilus strain BIM B-171     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1092292..1105801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG951_RS05435 (LG951_05435) lepB 1092803..1093315 (+) 513 WP_003212294.1 signal peptidase I -
  LG951_RS05440 (LG951_05440) - 1093350..1094495 (-) 1146 WP_003211629.1 AbrB family transcriptional regulator -
  LG951_RS05445 (LG951_05445) - 1094762..1095184 (+) 423 WP_003210872.1 BlaI/MecI/CopY family transcriptional regulator -
  LG951_RS05450 (LG951_05450) - 1095177..1096016 (+) 840 WP_050945367.1 M56 family metallopeptidase -
  LG951_RS05455 (LG951_05455) - 1096073..1096483 (+) 411 WP_003211332.1 DoxX family protein -
  LG951_RS05460 (LG951_05460) - 1096680..1097126 (-) 447 WP_226804977.1 ferritin-like domain-containing protein -
  LG951_RS05465 (LG951_05465) addB 1097292..1100801 (+) 3510 WP_226804979.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  LG951_RS05470 (LG951_05470) addA 1100779..1104477 (+) 3699 WP_226804981.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LG951_RS05475 (LG951_05475) sbcD 1104504..1105679 (+) 1176 WP_226804983.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134809.65 Da        Isoelectric Point: 5.2307

>NTDB_id=614802 LG951_RS05465 WP_226804979.1 1097292..1100801(+) (addB) [Bacillus pumilus strain BIM B-171]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEEHLQDEYVHSEDYLTLLSQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLYAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLAKECEADISENIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVGRHIDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IASPLYQLQKRLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDDEQLKFVANEH
VSLSYTISQLQQWLNQYPISGVWWSVYNYLMTSPHRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKLSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANIKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=614802 LG951_RS05465 WP_226804979.1 1097292..1100801(+) (addB) [Bacillus pumilus strain BIM B-171]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGTCTTGCTTGGTCGATTTTACAGCAGACTGGT
GGAGCGAATCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCGGAGGATATTGAGAAAATTTCAGCGTCTAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGGAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTTCTTTCCCAGCAAATTCCTTCAGCTGAAGAAATAAAAGGAGCACACATCTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCTTGCTGTATGCAGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCTAA
AGAGTGCGAAGCTGACATTTCTGAAAACATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCCGTTCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATCCTTGATTTAGTGAGAGAAAAAGGACTCAGACTGCGAGATATCTCGAT
TGTAGGGCGGCATATAGATGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTAATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTTGAAAACTATTGCATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGTT
TCCAATCATTAGATGAGGATTTCGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGCGTCCCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAAAGCGCAAAAGGTAAGGGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCCTTATTCCAACAGATGATAGATACAGGGTTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATTGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTACGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGTCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGAGAATGGTTAAAACGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCATCATTTATATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAGCTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACCATCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATCAGCGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTCATCGGGATGTATCAAAAAACATCATGTCAAGCTTGTTCTTTACAAATCGAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAGTTCAATGCG
TGTGCATTCTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATCGG
CCAATTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAGATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTTCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAATTGAG
TGGATTTTCTCCGGTAGGGCTTGAGTTAGGTTTTGGAGGTCAAGGGCCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTTGTCGGAAGGATTGACAGGGTAGATAAGGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGATCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCACTTGCAGAGGATGAAATTGAGCAGGAAATCTTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCGCAAGTCATTCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGTTCTGATTCGGCAGTTTTAAGTGAGGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATATCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.15

99.316

0.657