Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   N175_RS02715 Genome accession   NC_022223
Coordinates   588026..588628 (+) Length   200 a.a.
NCBI ID   WP_013855886.1    Uniprot ID   A0AAW4AFV0
Organism   Vibrio anguillarum M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 583026..593628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N175_RS02700 (N175_02800) oxyR 583451..584341 (+) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  N175_RS02705 (N175_02805) - 584378..586876 (-) 2499 WP_013855884.1 penicillin-binding protein 1A -
  N175_RS02710 (N175_02810) pilM 587026..588036 (+) 1011 WP_013855885.1 type IV pilus assembly protein PilM Machinery gene
  N175_RS02715 (N175_02815) pilN 588026..588628 (+) 603 WP_013855886.1 PilN domain-containing protein Machinery gene
  N175_RS02720 (N175_02820) pilO 588621..589214 (+) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  N175_RS02725 (N175_02825) pilP 589201..589722 (+) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  N175_RS02730 (N175_02830) pilQ 589746..591461 (+) 1716 WP_013855889.1 type IV pilus secretin PilQ Machinery gene
  N175_RS02735 (N175_02835) aroK 591648..592169 (+) 522 WP_010317691.1 shikimate kinase AroK -
  N175_RS02740 (N175_02840) aroB 592194..593282 (+) 1089 WP_026027894.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 22702.27 Da        Isoelectric Point: 9.6928

>NTDB_id=61355 N175_RS02715 WP_013855886.1 588026..588628(+) (pilN) [Vibrio anguillarum M3]
MLHSINLLPWREEQRAAHKRRFMGLVVLGVMSAVGIQWGIGLYFSGQAQLQESRLAYLNQYIQQLDKQINALKMTEQEHK
ALLTRLSVVESLQSQRNKTTELMNLMPQLMPEGVYVDKIKMNGEEIEITGISDSTARLATMLDNFEKSALLSDVNMHSIV
HGNKRFGKEFQTFKVSFMFHSPTPQGSVLTKIRPTGAVHD

Nucleotide


Download         Length: 603 bp        

>NTDB_id=61355 N175_RS02715 WP_013855886.1 588026..588628(+) (pilN) [Vibrio anguillarum M3]
ATGTTGCATAGCATTAACTTACTGCCTTGGCGAGAGGAACAAAGAGCGGCGCATAAGCGTCGTTTTATGGGCTTGGTCGT
CTTAGGTGTGATGAGCGCTGTTGGTATTCAATGGGGTATCGGTTTGTATTTCTCTGGGCAAGCACAGCTGCAAGAGTCTC
GTCTGGCCTATTTGAATCAATACATTCAGCAGCTTGATAAACAGATCAATGCATTAAAAATGACGGAACAAGAACACAAG
GCACTATTAACTCGCTTATCGGTTGTTGAGTCATTGCAAAGCCAACGCAATAAAACCACCGAGTTGATGAACCTGATGCC
ACAGCTTATGCCTGAAGGAGTCTACGTCGATAAGATCAAAATGAATGGTGAAGAAATTGAAATTACGGGCATCAGTGACA
GCACTGCGCGTCTTGCGACCATGCTCGATAATTTTGAAAAATCAGCCTTACTTTCCGATGTCAACATGCACTCTATCGTG
CACGGTAATAAGCGATTTGGTAAGGAGTTTCAGACGTTTAAAGTGTCATTTATGTTTCATTCCCCGACACCACAGGGCTC
TGTGCTAACCAAGATTCGGCCTACAGGAGCTGTCCATGACTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

67.553

94

0.635
  pilN Vibrio campbellii strain DS40M4

65.591

93

0.61

Multiple sequence alignment