Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LG401_RS11045 Genome accession   NZ_CP084711
Coordinates   2188748..2189818 (-) Length   356 a.a.
NCBI ID   WP_060596836.1    Uniprot ID   -
Organism   Bacillus pumilus strain AR03     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2183748..2194818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG401_RS10995 (LG401_10995) sipW 2183842..2184414 (-) 573 WP_066031752.1 signal peptidase I SipW -
  LG401_RS11000 (LG401_11000) tapA 2184466..2184993 (-) 528 WP_060596834.1 amyloid fiber anchoring/assembly protein TapA -
  LG401_RS11005 (LG401_11005) - 2185293..2185571 (+) 279 WP_223250494.1 DUF3889 domain-containing protein -
  LG401_RS11010 (LG401_11010) - 2185605..2185799 (-) 195 WP_041086171.1 YqzE family protein -
  LG401_RS11015 (LG401_11015) - 2185858..2186241 (-) 384 WP_034661288.1 competence type IV pilus minor pilin ComGG -
  LG401_RS11020 (LG401_11020) comGF 2186238..2186738 (-) 501 WP_034661289.1 competence type IV pilus minor pilin ComGF -
  LG401_RS11025 (LG401_11025) comGE 2186677..2186994 (-) 318 WP_034661291.1 competence type IV pilus minor pilin ComGE -
  LG401_RS11030 (LG401_11030) comGD 2186978..2187424 (-) 447 WP_066030666.1 competence type IV pilus minor pilin ComGD -
  LG401_RS11035 (LG401_11035) comGC 2187417..2187710 (-) 294 WP_106072454.1 competence type IV pilus major pilin ComGC Machinery gene
  LG401_RS11040 (LG401_11040) comGB 2187727..2188767 (-) 1041 WP_066030664.1 competence type IV pilus assembly protein ComGB -
  LG401_RS11045 (LG401_11045) comGA 2188748..2189818 (-) 1071 WP_060596836.1 competence type IV pilus ATPase ComGA Machinery gene
  LG401_RS11050 (LG401_11050) - 2189996..2190868 (-) 873 WP_034661297.1 STAS domain-containing protein -
  LG401_RS11060 (LG401_11060) - 2191092..2191472 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LG401_RS11065 (LG401_11065) - 2191686..2191928 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  LG401_RS11070 (LG401_11070) - 2191977..2192615 (-) 639 WP_060596838.1 MBL fold metallo-hydrolase -
  LG401_RS11075 (LG401_11075) - 2192773..2192946 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  LG401_RS11080 (LG401_11080) - 2192971..2193285 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -
  LG401_RS11085 (LG401_11085) - 2193363..2194616 (-) 1254 WP_060596687.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40267.63 Da        Isoelectric Point: 9.0692

>NTDB_id=612631 LG401_RS11045 WP_060596836.1 2188748..2189818(-) (comGA) [Bacillus pumilus strain AR03]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASIRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDLIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRSVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=612631 LG401_RS11045 WP_060596836.1 2188748..2189818(-) (comGA) [Bacillus pumilus strain AR03]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
AAAAGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGGTCACTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGGATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCATCAGATTATTATCATTTTTGA
ATCATTCGCATGGGCTGATTTTATTTACCGGTCCAACAAATTCAGGGAAAACAACAACCCTTTATTCATTAATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGTGCTATTTTAAGACATGATCCAGATCTGATTGTGCTAG
GAGAAATAAGAGATGCCGAAACGGCCCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACGCTTCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAT
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTAGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.124

99.438

0.638