Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LGQ02_RS00605 Genome accession   NZ_CP084703
Coordinates   127174..128580 (+) Length   468 a.a.
NCBI ID   WP_226516344.1    Uniprot ID   -
Organism   Bacillus shivajii strain JCM 32183     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 122174..133580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGQ02_RS00585 (LGQ02_00585) - 122447..122917 (+) 471 WP_226516340.1 CtsR family transcriptional regulator -
  LGQ02_RS00590 (LGQ02_00590) - 122943..123479 (+) 537 WP_226516341.1 UvrB/UvrC motif-containing protein -
  LGQ02_RS00595 (LGQ02_00595) - 123483..124574 (+) 1092 WP_226516342.1 protein arginine kinase -
  LGQ02_RS00600 (LGQ02_00600) clpC 124571..127015 (+) 2445 WP_226516343.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  LGQ02_RS00605 (LGQ02_00605) radA 127174..128580 (+) 1407 WP_226516344.1 DNA repair protein RadA Machinery gene
  LGQ02_RS00610 (LGQ02_00610) disA 128555..129637 (+) 1083 WP_226516345.1 DNA integrity scanning diadenylate cyclase DisA -
  LGQ02_RS00615 (LGQ02_00615) - 129851..130954 (+) 1104 WP_226516346.1 PIN/TRAM domain-containing protein -
  LGQ02_RS00620 (LGQ02_00620) ispD 130969..131661 (+) 693 WP_226516347.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LGQ02_RS00625 (LGQ02_00625) ispF 131661..132143 (+) 483 WP_226518173.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 468 a.a.        Molecular weight: 51026.77 Da        Isoelectric Point: 7.0295

>NTDB_id=612530 LGQ02_RS00605 WP_226516344.1 127174..128580(+) (radA) [Bacillus shivajii strain JCM 32183]
MAKKKTKYVCQECGYESMKWMGKCPGCQSWNTMVEEFAAVPTEKKRGFVTSPNAKTMKPQPITKIERKEENRYDTHINEL
NRVLGGGIVPGSLVLVGGDPGIGKSTLLLQVSAEMSKNKKRVLYISGEESVKQTKIRADRLGIASDELYVLAETDVEYIE
KAIEEIDPTLVIIDSIQTVYLDNVSSAPGSVSQVRECTAAFMRVAKTKGIAIFIVGHVTKQGSIAGPRLLEHMVDSVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMKEIGLEEVLNPSEIFLEERSSGAAGSAVVASMEGTRTVLVEIQSLISPTSF
GNPRRMATGIDHNRISLLMAVLEKRVGMLLQNSDAYVKVAGGVRLDEPSIDLSIAVAIASSFRNAPTSPTDVIIGEVGLT
GEVRRVSRVEQRVLEAEKLGFKRVIIPEKNIGGWSIPQGIDVVGVTTVNEALEVTLGGATSGSPKFEL

Nucleotide


Download         Length: 1407 bp        

>NTDB_id=612530 LGQ02_RS00605 WP_226516344.1 127174..128580(+) (radA) [Bacillus shivajii strain JCM 32183]
ATGGCGAAAAAGAAAACAAAGTATGTTTGCCAAGAGTGCGGATATGAATCGATGAAATGGATGGGGAAGTGTCCAGGGTG
CCAAAGTTGGAATACGATGGTAGAGGAGTTTGCAGCTGTACCAACAGAAAAAAAGAGAGGATTTGTTACATCCCCAAATG
CTAAAACAATGAAGCCCCAGCCGATAACAAAAATCGAACGAAAAGAAGAAAATCGATATGATACGCATATTAATGAATTA
AACCGTGTATTAGGTGGAGGGATCGTTCCAGGTTCTTTAGTCCTGGTTGGAGGGGATCCTGGAATAGGAAAATCCACTCT
TCTTTTACAAGTTTCTGCAGAAATGTCTAAAAACAAAAAAAGAGTACTATATATTTCAGGTGAAGAGTCTGTAAAGCAAA
CAAAGATTCGAGCGGACAGGTTAGGGATAGCAAGCGATGAGCTTTATGTTTTAGCTGAAACAGATGTGGAATATATAGAG
AAAGCGATCGAGGAAATTGATCCGACGCTTGTCATTATTGACTCGATTCAGACGGTTTATTTGGACAATGTAAGTAGTGC
ACCTGGCAGTGTTTCACAAGTTCGTGAATGTACGGCTGCATTTATGAGAGTGGCAAAAACAAAGGGAATTGCTATTTTTA
TCGTCGGGCATGTTACAAAGCAAGGTTCAATTGCTGGACCTCGCCTTTTAGAGCATATGGTTGATTCTGTCTTATATTTT
GAAGGAGAACGTCATCATACATATCGAATTCTACGAGCAGTAAAAAACCGGTTTGGGTCAACCAATGAAATAGGTATATT
TGAAATGAAGGAGATAGGGTTAGAAGAGGTACTAAATCCTTCTGAAATTTTCCTTGAAGAACGTTCATCCGGAGCGGCTG
GGTCAGCAGTTGTTGCTTCGATGGAAGGTACGAGGACGGTTTTAGTTGAAATTCAATCATTAATTTCACCGACGAGTTTT
GGAAACCCTAGAAGAATGGCAACAGGTATCGACCACAACCGGATTTCTTTACTAATGGCTGTTTTGGAAAAACGTGTAGG
TATGCTGTTACAAAATTCTGATGCATATGTAAAAGTTGCTGGTGGCGTCCGCCTTGATGAACCATCCATTGACTTATCTA
TTGCAGTTGCAATTGCATCTAGTTTTCGTAATGCACCAACTTCTCCTACTGATGTAATTATTGGAGAGGTTGGCTTAACA
GGAGAAGTAAGACGTGTCTCTAGAGTCGAGCAACGAGTACTAGAAGCAGAGAAACTAGGGTTTAAGCGAGTTATTATACC
AGAGAAGAATATCGGTGGGTGGTCAATTCCCCAAGGAATCGATGTCGTCGGTGTAACAACGGTAAACGAAGCTTTAGAAG
TGACGTTAGGAGGAGCAACGAGTGGATCACCAAAATTCGAATTATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(64-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.271

97.863

0.707
  radA Streptococcus pneumoniae Rx1

59.823

96.795

0.579
  radA Streptococcus pneumoniae D39

59.823

96.795

0.579
  radA Streptococcus pneumoniae R6

59.823

96.795

0.579
  radA Streptococcus pneumoniae TIGR4

59.823

96.795

0.579
  radA Streptococcus mitis NCTC 12261

59.823

96.795

0.579
  radA Streptococcus mitis SK321

59.823

96.795

0.579