Detailed information NTG0612340

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LGL96_RS03255
• Genome accession: NZ_CP084656
• Coordinates: 769968..770813 (-)
• Length: 281 a.a.
• NCBI ID: WP_044208476.1
• Uniprot ID: -
• Organism: Pectobacterium versatile strain SR1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 764968..775813

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGL96_RS03230 (LGL96_03230)	-	765145..765915 (-)	771	WP_103862227.1	ABC transporter ATP-binding protein	-
LGL96_RS03235 (LGL96_03235)	-	765917..766954 (-)	1038	WP_039492234.1	iron ABC transporter permease	-
LGL96_RS03240 (LGL96_03240)	-	766951..768015 (-)	1065	WP_211005771.1	ABC transporter substrate-binding protein	-
LGL96_RS03245 (LGL96_03245)	-	768367..769392 (+)	1026	WP_103184720.1	LLM class flavin-dependent oxidoreductase	-
LGL96_RS03250 (LGL96_03250)	ypdK	769748..769822 (+)	75	WP_099606229.1	membrane protein YpdK	-
LGL96_RS03255 (LGL96_03255)	pilD	769968..770813 (-)	846	WP_044208476.1	A24 family peptidase	Machinery gene
LGL96_RS03260 (LGL96_03260)	-	770903..771649 (-)	747	WP_103160336.1	type II secretion system protein N	-
LGL96_RS03265 (LGL96_03265)	gspM	771639..772133 (-)	495	WP_225586093.1	type II secretion system protein GspM	-
LGL96_RS03270 (LGL96_03270)	gspL	772130..773407 (-)	1278	WP_225586094.1	type II secretion system protein GspL	-
LGL96_RS03275 (LGL96_03275)	gspK	773404..774390 (-)	987	WP_039492241.1	type II secretion system minor pseudopilin GspK	-
LGL96_RS03280 (LGL96_03280)	gspJ	774387..775037 (-)	651	WP_103160335.1	type II secretion system minor pseudopilin GspJ	-
LGL96_RS03285 (LGL96_03285)	gspI	775037..775402 (-)	366	WP_044208462.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31323.24 Da
• Isoelectric Point: 6.6982

>NTDB_id=612340 LGL96_RS03255 WP_044208476.1 769968..770813(-) (pilD) [Pectobacterium versatile strain SR1]
MDDLREFAQLFPAWWFGALGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMADSEHDTRYNLWWPPSACPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSAQYPLVEVITMLAFLAAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWMGLLFNLSGTFVPLNDAVVGAMAGYLSLWLLYWAFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTAKPLAFGPWLAIGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=612340 LGL96_RS03255 WP_044208476.1 769968..770813(-) (pilD) [Pectobacterium versatile strain SR1]
GTGGACGATTTAAGGGAATTCGCACAGCTGTTTCCGGCGTGGTGGTTTGGGGCACTTGGCGTGTTGGGGTTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATGGCCGATTCCGAGCATGATACGCGCTACAACCTGTGGTGGCCGCCTTCGGCTTGCCCGCACTGCCAGCAGGCTATT
GCGGTAAAAGATAATATCCCGCTATTCAGCTGGCTGTGGCTGCGCGGCCGCTCCCGCTGCTGCCATCAATCGGTCTCTGC
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGGCGGCAGGTTTGCTCTGGTTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATCCTGCTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATGGGGTTGCTGTTTAATCTGTCGGGGACATTTGTCCCATTGAATGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTACTTTATTGGGCATTCAAATACGCAACGGGCAAAGAAGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCTGCGCTGGGGGCCTGGCTTGGCTGGCAGGCATTGCCGAATCTGGTTCTGGTGGCGGCGTTGAGC
GGGCTGGTCGTGACTCTCATCTGGCGCGGGTTGCGTAAAGAGGATACGGCTAAACCGTTAGCTTTTGGCCCCTGGCTGGC
AATCGGCGGGGTATTCGGCGTGATAATGAACGGGTTTAATCTGTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.292	96.441	0.495
pilD	Vibrio campbellii strain DS40M4	46.296	96.085	0.445
pilD	Neisseria gonorrhoeae MS11	47.328	93.238	0.441
pilD	Acinetobacter baumannii D1279779	42.857	97.153	0.416
pilD	Acinetobacter nosocomialis M2	42.491	97.153	0.413