Detailed information NTG0612335

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LGL97_RS14865
• Genome accession: NZ_CP084655
• Coordinates: 3332160..3333005 (+)
• Length: 281 a.a.
• NCBI ID: WP_225582976.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain SR10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3327160..3338005

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGL97_RS14835 (LGL97_14835)	gspI	3327552..3327917 (+)	366	WP_039284733.1	type II secretion system minor pseudopilin GspI	-
LGL97_RS14840 (LGL97_14840)	gspJ	3327917..3328567 (+)	651	WP_225582974.1	type II secretion system minor pseudopilin GspJ	-
LGL97_RS14845 (LGL97_14845)	gspK	3328564..3329550 (+)	987	WP_010681643.1	type II secretion system minor pseudopilin GspK	-
LGL97_RS14850 (LGL97_14850)	gspL	3329547..3330824 (+)	1278	WP_225582975.1	type II secretion system protein GspL	-
LGL97_RS14855 (LGL97_14855)	gspM	3330821..3331315 (+)	495	WP_039526285.1	type II secretion system protein GspM	-
LGL97_RS14860 (LGL97_14860)	-	3331305..3332051 (+)	747	WP_010681640.1	type II secretion system protein N	-
LGL97_RS14865 (LGL97_14865)	pilD	3332160..3333005 (+)	846	WP_225582976.1	A24 family peptidase	Machinery gene
LGL97_RS14870 (LGL97_14870)	ypdK	3333166..3333240 (-)	75	WP_099606229.1	membrane protein YpdK	-
LGL97_RS14875 (LGL97_14875)	-	3333599..3334624 (-)	1026	WP_225582977.1	LLM class flavin-dependent oxidoreductase	-
LGL97_RS14880 (LGL97_14880)	-	3334935..3335999 (+)	1065	WP_039316311.1	ABC transporter substrate-binding protein	-
LGL97_RS14885 (LGL97_14885)	-	3335996..3337015 (+)	1020	WP_225582978.1	iron ABC transporter permease	-
LGL97_RS14890 (LGL97_14890)	-	3337017..3337787 (+)	771	WP_225582979.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31399.31 Da
• Isoelectric Point: 6.4540

>NTDB_id=612335 LGL97_RS14865 WP_225582976.1 3332160..3333005(+) (pilD) [Pectobacterium brasiliense strain SR10]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMADSNPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLSDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLVWRGLRKEDTAKPLAFGPWLAIGGVFGVIMNGFNL

Nucleotide

• Length: 846 bp

>NTDB_id=612335 LGL97_RS14865 WP_225582976.1 3332160..3333005(+) (pilD) [Pectobacterium brasiliense strain SR10]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGTTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAACTTGAAACGG
GCATGGCAGATTCCAATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCATGCCCGCACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAGTTGGCTGTGGCTGCGTGGCCGCTCCCGCTGCTGTCATCAATCAGTATCTGT
GCAATATCCGTTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGCTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCATTCCTGTTGGTGCTGACCGTTATTGATATCAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAGTGATGCCGTAGTCGGCGC
GATGGCCGGTTATTTATCTCTGTGGCTGCTTTATTGGGTATTCAAATACGCAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTTGCTGCGCTGGGTGCCTGGCTGGGCTGGCAGGCATTGCCGAACCTGGTTCTGGTGGCGGCGCTGAGC
GGGTTGGTTGTTACTCTCGTCTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATAGGCGGTGTGTTTGGCGTGATAATGAACGGATTCAATCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.923	96.441	0.491
pilD	Vibrio campbellii strain DS40M4	45.353	95.73	0.434
pilD	Neisseria gonorrhoeae MS11	46.743	92.883	0.434
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402