Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NP444_RS12055 Genome accession   NZ_CP101911
Coordinates   2535392..2537119 (+) Length   575 a.a.
NCBI ID   WP_023084171.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NWRC-1223     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2530392..2542119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP444_RS12035 (NP444_12035) - 2531093..2532022 (-) 930 WP_043088612.1 LysR family transcriptional regulator -
  NP444_RS12040 (NP444_12040) - 2532045..2533019 (-) 975 WP_003097735.1 quinone oxidoreductase -
  NP444_RS12045 (NP444_12045) - 2533245..2533997 (+) 753 WP_003113396.1 class I SAM-dependent methyltransferase -
  NP444_RS12050 (NP444_12050) - 2534599..2535402 (+) 804 WP_033941953.1 ABC transporter permease -
  NP444_RS12055 (NP444_12055) pilF 2535392..2537119 (+) 1728 WP_023084171.1 GspE/PulE family protein Machinery gene
  NP444_RS12060 (NP444_12060) - 2537124..2538311 (+) 1188 WP_003097729.1 type II secretion system F family protein -
  NP444_RS12065 (NP444_12065) gspG 2538365..2538799 (+) 435 WP_033941951.1 type II secretion system major pseudopilin GspG -
  NP444_RS12070 (NP444_12070) - 2538768..2539178 (+) 411 WP_003097723.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NP444_RS12075 (NP444_12075) - 2539178..2539603 (+) 426 WP_003116709.1 type II secretion system protein J -
  NP444_RS12080 (NP444_12080) - 2539600..2540190 (+) 591 WP_003097720.1 type II secretion system protein J -
  NP444_RS12085 (NP444_12085) - 2540180..2541259 (+) 1080 WP_003122597.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63694.78 Da        Isoelectric Point: 5.3299

>NTDB_id=612233 NP444_RS12055 WP_023084171.1 2535392..2537119(+) (pilF) [Pseudomonas aeruginosa strain NWRC-1223]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TQAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=612233 NP444_RS12055 WP_023084171.1 2535392..2537119(+) (pilF) [Pseudomonas aeruginosa strain NWRC-1223]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTGGGAGGTATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCACTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGTTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGTTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTC
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCGGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACCACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAATACCAGGTGCCCAATATCACCCAGGTCCAGGTCCATG
CCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGCGCCTTCACGCGTTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCCAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.519

98.435

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.801

98.609

0.383
  pilF Neisseria gonorrhoeae MS11

36.842

99.13

0.365