NTDB

Overview

Name	comFA	Type	Machinery gene
Locus tag	LGO15_RS21835	Genome accession	NZ_CP084622
Coordinates	4264127..4265542 (-)	Length	471 a.a.
NCBI ID	WP_226087958.1	Uniprot ID	-
Organism	Mesobacillus sp. S13
Function	ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4259127..4270542

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGO15_RS21805	flgL	4259651..4260541 (-)	891	WP_226085907.1	flagellar hook-associated protein FlgL	-
LGO15_RS21810	flgK	4260552..4262099 (-)	1548	WP_226085908.1	flagellar hook-associated protein FlgK	-
LGO15_RS21815	-	4262117..4262587 (-)	471	WP_226085909.1	flagellar protein FlgN	-
LGO15_RS21820	flgM	4262644..4262913 (-)	270	WP_226085910.1	flagellar biosynthesis anti-sigma factor FlgM	-
LGO15_RS21825	-	4262992..4263393 (-)	402	WP_226085911.1	TIGR03826 family flagellar region protein	-
LGO15_RS21830	-	4263423..4264130 (-)	708	WP_226085912.1	ComF family protein	-
LGO15_RS21835	comFA	4264127..4265542 (-)	1416	WP_226087958.1	DEAD/DEAH box helicase	Machinery gene
LGO15_RS21840	-	4265995..4266834 (-)	840	WP_226085913.1	DegV family protein	-
LGO15_RS21845	sda	4267369..4267530 (-)	162	WP_226085914.1	sporulation histidine kinase inhibitor Sda	-
LGO15_RS21850	-	4267669..4268352 (-)	684	WP_167833218.1	response regulator	-
LGO15_RS21855	-	4268426..4269568 (-)	1143	WP_226085916.1	sensor histidine kinase	-

Sequence

Protein

Download Length: 471 a.a. Molecular weight: 53323.01 Da Isoelectric Point: 9.6427

                                >NTDB_id=612029 LGO15_RS21835 WP_226087958.1 4264127..4265542(-) (comFA) [Mesobacillus sp. S13]

MELPQLPPLSTNRSFHYNPELQRLLTGKQLLLEDLPFLMEEIQKHHELSYLAYRKGVKQKPLSCTRCGTTDPSWFAEFPC

SRCGKADFYCRKCLMMGRVSECTSLVSWAGPEPGFDQVTEPLQWRGELSEGQKAASLRVVEAVENSSELLVWAVAGAGKT

EVLFAGIAQALSTGKRVCVATPRTDVVLELAPRLKKVFPNIKIAALYGGSEDRHEYAQLTIATTHQLLRFYRSFDVMIVD

EVDAFPYSIDETLQYAVQQSRKPTSSLIYLTATPKRQWQKECRLGKRNYVTIPARFHRHPLPVPQFVWSGNWEKKLSKGK

LPPNIMKWIEARLARGKQALIFAPKIKSIDKILTLFRQLHPLIEMVHAEDPDRKEKVMKFRNKEIPILLTTTILERGVTI

PNIDVAVIGSEDRVFTESALVQIAGRAGRSAEYPTGSVTFFHYGKTEAMLSARSQIMMMNKEGIKKGLIDV

Nucleotide

Download Length: 1416 bp

                                >NTDB_id=612029 LGO15_RS21835 WP_226087958.1 4264127..4265542(-) (comFA) [Mesobacillus sp. S13]

ATGGAACTTCCGCAGTTGCCCCCGTTATCCACAAATCGCTCTTTCCACTACAATCCTGAACTACAAAGATTGCTGACTGG

AAAACAGCTGCTATTGGAGGATTTGCCTTTTTTGATGGAAGAAATACAGAAGCACCATGAACTAAGTTATTTAGCATACC

GCAAGGGAGTTAAGCAGAAGCCACTTTCCTGTACAAGATGCGGTACGACAGATCCTTCATGGTTTGCGGAGTTTCCCTGT

TCGCGTTGTGGAAAAGCAGATTTCTATTGCCGCAAGTGTTTGATGATGGGGAGGGTGAGTGAATGCACGTCGCTTGTCAG

CTGGGCTGGACCGGAACCGGGGTTTGATCAAGTGACGGAGCCACTTCAGTGGAGAGGTGAATTGTCAGAAGGGCAGAAGG

CTGCTTCTCTAAGAGTTGTGGAAGCTGTGGAAAACTCATCAGAGCTGCTTGTCTGGGCAGTTGCAGGGGCAGGTAAAACG

GAAGTGTTATTTGCCGGGATTGCACAGGCTTTATCCACAGGCAAACGGGTATGTGTGGCTACTCCTAGGACGGATGTGGT

TTTGGAATTGGCACCTCGATTGAAAAAAGTGTTTCCAAATATAAAGATTGCTGCTCTCTATGGCGGCAGTGAAGACCGTC

ATGAGTATGCCCAGCTTACCATCGCCACAACGCATCAGCTGCTTCGCTTTTACAGGTCGTTCGACGTGATGATTGTGGAT

GAAGTCGATGCCTTTCCTTATTCAATTGACGAAACTCTCCAATATGCCGTTCAGCAATCCAGAAAACCAACTTCCTCGTT

GATTTACTTAACTGCCACCCCTAAAAGACAATGGCAGAAGGAATGTCGTTTAGGAAAAAGAAATTATGTTACGATTCCTG

CCCGCTTTCATAGGCATCCGCTGCCGGTACCACAGTTTGTGTGGTCCGGGAACTGGGAGAAAAAACTGTCTAAAGGGAAA

CTTCCTCCAAATATTATGAAATGGATAGAAGCTAGATTAGCCAGAGGCAAACAAGCACTGATTTTTGCTCCAAAAATCAA

ATCCATAGACAAAATCCTCACCCTCTTTCGTCAATTGCATCCTCTCATTGAAATGGTCCATGCAGAAGATCCTGACAGAA

AAGAAAAAGTCATGAAGTTTCGAAACAAAGAAATCCCCATCTTGCTTACCACAACCATCCTTGAACGAGGTGTTACGATC

CCTAATATCGATGTTGCCGTGATCGGGTCGGAGGACAGGGTTTTTACCGAGAGCGCTCTCGTTCAGATTGCCGGGCGGGC

AGGAAGGAGTGCTGAGTATCCGACAGGGAGTGTTACGTTTTTTCATTATGGTAAAACCGAAGCGATGCTCAGTGCCAGAA

GCCAGATTATGATGATGAATAAAGAAGGAATCAAGAAAGGGCTGATTGATGTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(127-276)

Helicase conserved C-terminal domain (Helicase_C)

(333-430)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comFA	Bacillus subtilis subsp. subtilis str. 168	49.032	98.726	0.484
comFA/cflA	Streptococcus mitis NCTC 12261	39.333	95.541	0.376
comFA/cflA	Streptococcus pneumoniae Rx1	39.813	90.658	0.361
comFA/cflA	Streptococcus pneumoniae D39	39.813	90.658	0.361
comFA/cflA	Streptococcus pneumoniae R6	39.813	90.658	0.361

Detailed information NTG0612029