Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LGO15_RS16750 Genome accession   NZ_CP084622
Coordinates   3263458..3264534 (-) Length   358 a.a.
NCBI ID   WP_226085336.1    Uniprot ID   -
Organism   Mesobacillus sp. S13     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3258458..3269534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGO15_RS16705 - 3258940..3259728 (+) 789 WP_226085330.1 YqhG family protein -
  LGO15_RS16710 - 3259782..3259964 (-) 183 WP_167831536.1 YqzE family protein -
  LGO15_RS16715 - 3260006..3260506 (-) 501 WP_226085331.1 shikimate kinase -
  LGO15_RS16720 comGG 3260523..3260846 (-) 324 WP_226087919.1 competence type IV pilus minor pilin ComGG -
  LGO15_RS16725 comGF 3260894..3261439 (-) 546 WP_318999791.1 competence type IV pilus minor pilin ComGF -
  LGO15_RS16730 - 3261321..3261656 (-) 336 WP_226085333.1 hypothetical protein -
  LGO15_RS16735 comGD 3261640..3262092 (-) 453 WP_226085334.1 competence type IV pilus minor pilin ComGD -
  LGO15_RS16740 comGC 3262076..3262402 (-) 327 WP_226087920.1 competence type IV pilus major pilin ComGC -
  LGO15_RS16745 comGB 3262440..3263471 (-) 1032 WP_226085335.1 competence type IV pilus assembly protein ComGB -
  LGO15_RS16750 comGA 3263458..3264534 (-) 1077 WP_226085336.1 competence type IV pilus ATPase ComGA Machinery gene
  LGO15_RS16760 - 3264777..3265130 (-) 354 WP_226085337.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LGO15_RS16765 - 3265325..3266026 (+) 702 WP_167831544.1 helix-turn-helix transcriptional regulator -
  LGO15_RS16770 - 3266106..3266351 (+) 246 WP_023627332.1 DUF2626 domain-containing protein -
  LGO15_RS16775 - 3266420..3267508 (-) 1089 WP_226085338.1 class I SAM-dependent methyltransferase -
  LGO15_RS16780 - 3267677..3268312 (-) 636 WP_226085339.1 MBL fold metallo-hydrolase -
  LGO15_RS16785 - 3268497..3268976 (+) 480 WP_167831547.1 hypothetical protein -
  LGO15_RS16790 - 3269088..3269261 (+) 174 WP_167831548.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 40001.97 Da        Isoelectric Point: 7.7404

>NTDB_id=612019 LGO15_RS16750 WP_226085336.1 3263458..3264534(-) (comGA) [Mesobacillus sp. S13]
MIIVKSIEQLADNVLKDALRSGASDVHIIPREKDTLIKFRLGNQLLPRYTLEQADCERLISHFKFTASMDIGEKRRPQSG
AFTYQFQEMKIGLRISTLPAYQSESMVIRLLPEQNQSPSFQISLFPSTSKKLISLLKHAHGLIIFTGPTGSGKTTTLYSM
LSETSDIVNRNVITLEDPIEKPSDTVLQVQVNERAGISYSAGLKAILRHDPDIIMVGEIRDKETAETAIRASLTGHLVLT
TMHTRDARGAIYRLIEFGINWLEIEQTLIAVTAQRLVELTCPYCEGTCSPFCYSSGSGKRGNVFEILTGKDLSAVLKEAK
GESQSYHYKTLKDAIRKGIALGFIKESEYQRWVLNDGE

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=612019 LGO15_RS16750 WP_226085336.1 3263458..3264534(-) (comGA) [Mesobacillus sp. S13]
ATGATTATTGTCAAATCAATTGAACAGCTAGCGGACAATGTCCTGAAAGATGCTTTAAGAAGTGGCGCGTCGGATGTACA
TATTATCCCTCGCGAGAAAGACACACTGATTAAGTTCAGACTAGGCAATCAGCTACTCCCCCGGTACACACTTGAACAGG
CTGATTGTGAGCGCCTCATTTCCCACTTCAAATTCACAGCTTCAATGGATATTGGTGAAAAAAGACGTCCTCAAAGCGGT
GCTTTCACGTATCAATTCCAAGAAATGAAGATCGGCTTAAGGATTTCCACGCTTCCAGCCTACCAGAGCGAAAGCATGGT
CATACGACTCCTTCCCGAACAAAATCAATCTCCTTCTTTCCAAATTTCTTTATTTCCATCCACATCGAAAAAACTGATTT
CCCTTTTAAAACACGCACATGGTTTAATCATTTTTACTGGCCCCACCGGCAGTGGAAAGACGACTACCTTGTATTCCATG
CTTTCCGAAACTTCGGATATAGTCAATCGCAATGTCATTACACTTGAAGATCCGATTGAAAAACCGAGTGATACAGTTCT
CCAGGTCCAGGTCAATGAACGGGCTGGCATTTCATATTCTGCAGGCTTGAAGGCCATACTCAGGCACGACCCGGATATTA
TCATGGTTGGGGAAATTCGGGATAAAGAGACCGCGGAAACGGCTATTAGGGCCTCGCTTACAGGGCATCTCGTACTGACG
ACGATGCATACAAGGGATGCGAGAGGAGCGATTTACAGGCTTATTGAATTCGGAATCAATTGGCTTGAAATTGAACAGAC
ATTGATTGCAGTGACGGCACAGAGGCTCGTTGAATTGACTTGTCCTTACTGTGAAGGAACTTGTTCGCCATTTTGCTATT
CATCGGGCAGCGGAAAAAGAGGAAATGTCTTCGAAATCCTAACGGGGAAGGATTTATCTGCTGTACTGAAGGAAGCAAAG
GGGGAGAGCCAAAGCTATCATTACAAGACGCTCAAAGATGCGATCCGGAAAGGAATCGCTCTGGGGTTCATCAAGGAGTC
CGAATACCAGAGGTGGGTTCTCAATGATGGAGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.337

99.441

0.55
  pilB Glaesserella parasuis strain SC1401

38.968

97.486

0.38
  ctsE Campylobacter jejuni subsp. jejuni 81-176

36.857

97.765

0.36