Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BASU_RS12085 Genome accession   NC_022081
Coordinates   2492779..2493393 (-) Length   204 a.a.
NCBI ID   WP_020956097.1    Uniprot ID   -
Organism   Bacillus velezensis UCMB5113     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2487779..2498393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BASU_RS12060 (BASU_2308) rpsT 2488082..2488348 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  BASU_RS12065 (BASU_2309) holA 2488365..2489406 (-) 1042 Protein_2323 DNA polymerase III subunit delta -
  BASU_RS20205 - 2489464..2489597 (-) 134 Protein_2324 hypothetical protein -
  BASU_RS19840 - 2489638..2489772 (+) 135 WP_003152870.1 YqzM family protein -
  BASU_RS12075 (BASU_2310) comEC 2489791..2492142 (-) 2352 WP_007408265.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BASU_RS12080 (BASU_2311) - 2492143..2492712 (-) 570 WP_007408264.1 ComE operon protein 2 -
  BASU_RS12085 (BASU_2312) comEA 2492779..2493393 (-) 615 WP_020956097.1 helix-hairpin-helix domain-containing protein Machinery gene
  BASU_RS12090 (BASU_2313) comER 2493452..2494273 (+) 822 WP_020956098.1 late competence protein ComER -
  BASU_RS12095 (BASU_2314) - 2494343..2495080 (-) 738 WP_015240247.1 class I SAM-dependent methyltransferase -
  BASU_RS12100 (BASU_2315) rsfS 2495077..2495433 (-) 357 WP_007408260.1 ribosome silencing factor -
  BASU_RS12105 (BASU_2316) yqeK 2495451..2496011 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BASU_RS12110 (BASU_2317) - 2496001..2496570 (-) 570 WP_032866687.1 nicotinate-nucleotide adenylyltransferase -
  BASU_RS12115 (BASU_2318) yhbY 2496581..2496871 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  BASU_RS12120 (BASU_2319) aroE 2496865..2497707 (-) 843 WP_007408256.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22251.45 Da        Isoelectric Point: 6.4940

>NTDB_id=61125 BASU_RS12085 WP_020956097.1 2492779..2493393(-) (comEA) [Bacillus velezensis UCMB5113]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=61125 BASU_RS12085 WP_020956097.1 2492779..2493393(-) (comEA) [Bacillus velezensis UCMB5113]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACCGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363

Multiple sequence alignment