Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LE165_RS01405 Genome accession   NZ_CP084434
Coordinates   305141..306115 (-) Length   324 a.a.
NCBI ID   WP_084951767.1    Uniprot ID   -
Organism   Staphylococcus lugdunensis strain RMLUG2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300141..311115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LE165_RS01375 (LE165_01390) gcvT 300715..301806 (-) 1092 WP_037544364.1 glycine cleavage system aminomethyltransferase GcvT -
  LE165_RS01380 (LE165_01395) - 301976..302494 (-) 519 WP_002459021.1 shikimate kinase -
  LE165_RS12780 (LE165_01400) - 302648..303106 (-) 459 WP_014533299.1 competence type IV pilus minor pilin ComGF -
  LE165_RS01385 (LE165_01405) - 303069..303362 (-) 294 WP_002459018.1 hypothetical protein -
  LE165_RS01390 (LE165_01410) comGD 303349..303843 (-) 495 WP_225306455.1 competence type IV pilus minor pilin ComGD -
  LE165_RS01395 (LE165_01415) comGC 303776..304090 (-) 315 WP_002459016.1 competence type IV pilus major pilin ComGC Machinery gene
  LE165_RS01400 (LE165_01420) comGB 304105..305169 (-) 1065 WP_252920573.1 competence type IV pilus assembly protein ComGB -
  LE165_RS01405 (LE165_01425) comGA 305141..306115 (-) 975 WP_084951767.1 competence type IV pilus ATPase ComGA Machinery gene
  LE165_RS01410 (LE165_01430) - 306162..306785 (-) 624 WP_084951765.1 MBL fold metallo-hydrolase -
  LE165_RS01415 (LE165_01435) - 306789..307112 (-) 324 WP_084951763.1 MTH1187 family thiamine-binding protein -
  LE165_RS01420 (LE165_01440) - 307112..308098 (-) 987 WP_084951762.1 ROK family glucokinase -
  LE165_RS01425 (LE165_01445) - 308095..308298 (-) 204 WP_002459010.1 YqgQ family protein -
  LE165_RS01430 (LE165_01450) - 308282..309739 (-) 1458 WP_037544370.1 rhomboid family protein -
  LE165_RS01435 (LE165_01455) - 309756..310292 (-) 537 WP_084951760.1 5-formyltetrahydrofolate cyclo-ligase -
  LE165_RS01440 (LE165_01460) rpmG 310542..310691 (-) 150 WP_001830957.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36948.80 Da        Isoelectric Point: 7.8896

>NTDB_id=611231 LE165_RS01405 WP_084951767.1 305141..306115(-) (comGA) [Staphylococcus lugdunensis strain RMLUG2]
MKILFKEIVNKAIQQNASDIHFIPSQTTVIIKFRVNDQLVDYEELNLSIYTKLLTFMKFQAGLDVSTHQIAQSGRYTYQY
KQQYYLRISTLPLSLGTESCVIRIVPQYFQQHKTCYQFNDFKQFMNKKQGLLLFSGPTGSGKSTLMYQMVLYAQQELNLN
VITIEDPVEQLISGITQVSVNDKAGINYVSTFKAILRCDPDVILIGEIRDASIAKCVIQASLSGHLVLSTIHANNCKGTL
LRLLEMGISVQEICQSINLISNQRLITTLNQQRALVCEMMINHQIEYFFEHNHTLLKSFHHLSQLIGNMSKEGVICEETA
NKYT

Nucleotide


Download         Length: 975 bp        

>NTDB_id=611231 LE165_RS01405 WP_084951767.1 305141..306115(-) (comGA) [Staphylococcus lugdunensis strain RMLUG2]
ATGAAAATATTATTTAAGGAAATTGTTAACAAAGCGATACAACAGAATGCAAGTGATATTCACTTTATTCCAAGTCAAAC
TACTGTCATCATTAAATTTCGTGTTAATGACCAACTTGTAGATTATGAAGAATTAAATTTAAGCATCTATACTAAACTGT
TAACGTTTATGAAATTTCAAGCTGGCTTAGATGTATCAACACATCAAATCGCCCAAAGTGGAAGATATACCTATCAATAT
AAGCAACAATATTACTTAAGAATCTCAACTTTACCACTATCATTAGGTACAGAAAGTTGCGTCATTCGTATCGTACCACA
ATACTTTCAACAACATAAAACGTGTTACCAATTTAATGATTTCAAACAGTTTATGAATAAAAAGCAAGGATTGTTATTAT
TTAGTGGTCCAACTGGATCTGGTAAAAGTACACTGATGTATCAAATGGTTTTATATGCACAACAAGAGCTTAATTTAAAT
GTTATTACAATTGAAGATCCAGTTGAACAGTTGATATCAGGCATTACACAAGTATCTGTAAATGATAAAGCAGGCATTAA
TTATGTATCAACATTTAAAGCTATTCTGCGCTGTGATCCTGATGTTATCTTAATTGGCGAAATTAGAGATGCCAGTATCG
CAAAATGTGTTATTCAGGCAAGTTTAAGTGGCCATTTAGTATTATCTACAATTCACGCAAACAACTGCAAAGGAACTTTA
TTAAGATTATTAGAAATGGGAATTTCAGTTCAAGAAATATGTCAATCAATTAATTTAATCTCTAATCAAAGACTCATTAC
CACCCTAAATCAACAACGAGCATTGGTTTGCGAAATGATGATAAATCATCAAATAGAATACTTTTTTGAACATAATCATA
CCTTGCTTAAATCATTTCATCATTTATCACAGTTAATTGGTAACATGTCAAAAGAAGGGGTCATTTGTGAAGAAACTGCT
AATAAATATACTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

70.898

99.691

0.707
  comGA Staphylococcus aureus N315

70.898

99.691

0.707